Strumpellin (WASHC5) (NM_014846) Human Tagged ORF Clone

SKU RG210620

KIAA0196 (tGFP-tagged) - Human KIAA0196 (KIAA0196)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Strumpellin
• Synonyms: KIAA0196; RTSC; RTSC1; SPG8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210620 representing NM_014846; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTGGACTTTCTAGCCGAGAACAACCTCTGTGGCCAAGCAATCCTAAGGATTGTTTCCTGTGGTAATG; CCATCATTGCTGAACTTTTGAGACTCTCTGAGTTTATTCCTGCTGTGTTCAGGTTAAAAGACAGAGCTGA; TCAACAGAAATATGGAGATATCATATTTGATTTCAGCTATTTTAAGGGTCCAGAATTATGGGAAAGCAAA; CTGGATGCTAAGCCAGAGCTACAGGATTTAGATGAAGAATTTCGTGAAAACAACATAGAAATTGTGACCA; GATTTTATTTAGCATTTCAAAGTGTACATAAATATATTGTAGACTTAAACAGATATCTAGATGATCTCAA; TGAAGGGGTTTATATTCAGCAAACCTTAGAAACTGTGCTTCTCAATGAAGATGGAAAACAACTTCTATGT; GAAGCACTGTACTTATATGGAGTTATGCTACTGGTCATTGACCAAAAGATTGAAGGAGAAGTCAGAGAGA; GGATGCTGGTTTCTTACTACCGATACAGTGCTGCTCGATCTTCTGCTGATTCAAATATGGACGATATTTG; TAAGCTGCTTCGAAGTACAGGTTATTCTAGCCAACCAGGTGCCAAAAGACCATCCAACTATCCCGAGAGC; TATTTCCAGAGAGTGCCTATCAACGAATCCTTCATCAGTATGGTCATTGGTCGACTGAGATCTGATGATA; TTTACAACCAGGTCTCAGCGTATCCTTTGCCGGAGCATCGCAGCACAGCCCTGGCAAACCAAGCTGCCAT; GCTGTACGTGATTCTCTACTTTGAGCCTTCCATCCTTCACACCCATCAAGCAAAAATGAGAGAGATAGTG; GATAAATACTTTCCAGATAATTGGGTAATTAGTATTTACATGGGGATCACAGTTAATCTAGTAGATGCTT; GGGAACCTTACAAAGCTGCAAAAACTGCTTTAAATAATACCCTGGACCTTTCAAATGTCAGAGAACAGGC; AAGCAGATATGCTACTGTCAGTGAAAGAGTGCATGCTCAAGTGCAGCAATTTCTAAAAGAAGGTTATTTA; AGGGAGGAGATGGTTCTGGACAATATCCCAAAGCTTCTGAACTGCCTGAGAGACTGCAATGTTGCCATCC; GATGGCTGATGCTTCATACAGCAGACTCAGCCTGTGACCCAAACAACAAACGCCTTCGTCAAATCAAGGA; CCAGATTCTAACAGACTCTCGGTACAATCCCAGGATCCTCTTCCAGCTGCTGTTAGATACTGCACAATTT; GAGTTTATACTCAAAGAGATGTTCAAGCAAATGCTTTCAGAAAAGCAAACCAAATGGGAGCATTACAAGA; AAGAGGGTTCGGAGCGGATGACTGAGCTTGCTGATGTCTTTTCAGGAGTGAAACCCCTAACCAGAGTGGA; GAAAAATGAAAACCTTCAAGCTTGGTTCAGAGAGATCTCAAAACAAATATTGTCTTTAAATTATGATGAT; TCTACTGCTGCGGGCAGAAAAACTGTACAACTGATACAAGCTTTGGAAGAGGTTCAAGAATTCCACCAGT; TGGAATCCAATCTGCAAGTATGTCAGTTTCTTGCCGATACTCGAAAGTTTCTTCATCAAATGATCAGAAC; CATTAACATTAAAGAGGAGGTTCTGATCACAATGCAGATCGTTGGGGACCTTTCTTTCGCTTGGCAGTTG; ATTGACAGTTTCACATCCATCATGCAAGAAAGCATAAGGGTAAATCCATCCATGGTTACTAAACTCAGAG; CTACCTTCCTAAAGCTTGCCTCTGCCCTCGATCTGCCCCTTCTTCGTATTAATCAGGCAAATAGCCCCGA; CCTGCTCAGCGTGTCACAGTACTATTCTGGAGAGTTGGTATCCTATGTGAGAAAAGTTTTGCAGATCATC; CCAGAAAGCATGTTTACATCTCTTCTAAAGATCATAAAGCTTCAGACCCACGACATTATTGAAGTGCCTA; CCCGCCTGGACAAAGACAAGCTGAGGGACTATGCTCAGCTAGGCCCACGATACGAGGTTGCCAAGCTTAC; TCATGCTATTTCCATTTTTACTGAAGGCATCTTAATGATGAAAACGACTTTGGTTGGCATCATCAAGGTG; GATCCAAAGCAGTTGCTGGAAGATGGAATAAGGAAAGAGCTTGTGAAGCGCGTTGCCTTTGCCCTGCATA; GGGGACTGATATTCAACCCTCGAGCCAAGCCAAGTGAATTGATGCCCAAGCTGAAAGAGTTGGGAGCGAC; CATGGATGGATTCCATCGTTCTTTTGAATACATACAGGACTATGTCAACATTTATGGTCTGAAGATTTGG; CAGGAAGAAGTATCTCGTATCATAAATTACAACGTGGAGCAAGAGTGTAATAACTTTCTAAGAACGAAGA; TTCAAGATTGGCAAAGCATGTACCAGTCCACTCATATTCCAATACCCAAGTTTACCCCTGTGGATGAGTC; TGTAACGTTTATTGGTCGACTCTGCAGAGAAATCCTGCGGATCACAGACCCAAAAATGACATGTCACATA; GACCAGCTGAACACTTGGTATGATATGAAAACTCATCAGGAAGTGACCAGCAGCCGCCTCTTCTCAGAAA; TCCAGACCACCTTGGGAACCTTTGGTCTAAATGGCTTAGACAGGCTTCTGTGCTTTATGATTGTAAAAGA; GTTACAGAATTTCCTCAGTATGTTTCAGAAAATTATCCTGAGAGACAGAACTGTTCAGGACACTTTAAAA; ACCCTCATGAATGCTGTCAGTCCCCTAAAAAGTATTGTCGCAAATTCAAATAAAATTTATTTTTCCGCCA; TTGCCAAAACACAGAAGATTTGGACTGCGTATCTCGAGGCTATAATGAAGGTTGGGCAGATGCAGATTCT; GAGACAACAGATTGCCAATGAATTAAATTATTCTTGTCGGTTTGATTCTAAACATCTGGCAGCTGCTCTG; GAGAATCTCAATAAGGCTCTCCTAGCAGACATTGAAGCCCACTATCAGGACCCTTCACTTCCTTACCCCA; AAGAAGATAACACACTTTTATATGAAATCACAGCCTATCTGGAGGCAGCTGGCATTCACAACCCACTGAA; TAAGATATACATAACAACAAAGCGCTTACCCTATTTTCCAATTGTAAACTTTCTATTTTTGATCGCTCAG; TTGCCAAAACTTCAATACAACAAAAATCTGGGAATGGTCTGCCGAAAACCGACCGACCCGGTTGATTGGC; CACCACTTGTCCTGGGACTGCTCACTCTGCTGAAGCAGTTCCATTCCCGGTACACCGAGCAGTTCCTGGC; GCTGATTGGCCAGTTTATCTGCTCCACGGTGGAGCAGTGTACAAGCCAGAAGATACCTGAAATTCCTGCA; GATGTTGTGGGTGCCCTTCTGTTCCTGGAGGATTATGTTCGGTACACAAAGCTACCCAGGAGGGTTGCTG; AAGCACATGTGCCTAATTTCATTTTTGATGAGTTCAGAACAGTGCTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210620 representing NM_014846; Red=Cloning site Green=Tags(s); ; MLDFLAENNLCGQAILRIVSCGNAIIAELLRLSEFIPAVFRLKDRADQQKYGDIIFDFSYFKGPELWESK; LDAKPELQDLDEEFRENNIEIVTRFYLAFQSVHKYIVDLNRYLDDLNEGVYIQQTLETVLLNEDGKQLLC; EALYLYGVMLLVIDQKIEGEVRERMLVSYYRYSAARSSADSNMDDICKLLRSTGYSSQPGAKRPSNYPES; YFQRVPINESFISMVIGRLRSDDIYNQVSAYPLPEHRSTALANQAAMLYVILYFEPSILHTHQAKMREIV; DKYFPDNWVISIYMGITVNLVDAWEPYKAAKTALNNTLDLSNVREQASRYATVSERVHAQVQQFLKEGYL; REEMVLDNIPKLLNCLRDCNVAIRWLMLHTADSACDPNNKRLRQIKDQILTDSRYNPRILFQLLLDTAQF; EFILKEMFKQMLSEKQTKWEHYKKEGSERMTELADVFSGVKPLTRVEKNENLQAWFREISKQILSLNYDD; STAAGRKTVQLIQALEEVQEFHQLESNLQVCQFLADTRKFLHQMIRTINIKEEVLITMQIVGDLSFAWQL; IDSFTSIMQESIRVNPSMVTKLRATFLKLASALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQII; PESMFTSLLKIIKLQTHDIIEVPTRLDKDKLRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKV; DPKQLLEDGIRKELVKRVAFALHRGLIFNPRAKPSELMPKLKELGATMDGFHRSFEYIQDYVNIYGLKIW; QEEVSRIINYNVEQECNNFLRTKIQDWQSMYQSTHIPIPKFTPVDESVTFIGRLCREILRITDPKMTCHI; DQLNTWYDMKTHQEVTSSRLFSEIQTTLGTFGLNGLDRLLCFMIVKELQNFLSMFQKIILRDRTVQDTLK; TLMNAVSPLKSIVANSNKIYFSAIAKTQKIWTAYLEAIMKVGQMQILRQQIANELNYSCRFDSKHLAAAL; ENLNKALLADIEAHYQDPSLPYPKEDNTLLYEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQ; LPKLQYNKNLGMVCRKPTDPVDWPPLVLGLLTLLKQFHSRYTEQFLALIGQFICSTVEQCTSQKIPEIPA; DVVGALLFLEDYVRYTKLPRRVAEAHVPNFIFDEFRTVL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014846
• ORF Size: 3477 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014846.4
• RefSeq Size: 4173 bp
• RefSeq ORF: 3480 bp
• Locus ID: 9897
• UniProt ID: Q12768
• Cytogenetics: 8q24.13
• Summary: This gene encodes a 134 kDa protein named strumpellin that is predicted to have multiple transmembrane domains and a spectrin-repeat-containing domain. This ubiquitously expressed gene has its highest expression in skeletal muscle. The protein is named for Strumpell disease; a form of hereditary spastic paraplegia (HSP). Spastic paraplegias are a diverse group of disorders in which the autosomal dominant forms are characterized by progressive, lower extremity spasticity caused by axonal degeneration in the terminal portions of the longest descending and ascending corticospinal tracts. More than 30 loci (SPG1-33) have been implicated in hereditary spastic paraplegia diseases. [provided by RefSeq, Aug 2009]