Kv1.8 (KCNA10) (NM_005549) Human Tagged ORF Clone

SKU RG210392

KCNA10 (tGFP-tagged) - Human potassium voltage-gated channel, shaker-related subfamily, member 10 (KCNA10)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Kv1.8
• Synonyms: Kcn1; Kv1.8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210392 representing NM_005549; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATGTGTGTGGCTGGAAAGAAATGGAGGTTGCGCTGGTCAATTTTGATAATTCAGATGAAATCCAAG; AAGAGCCAGGCTATGCCACAGACTTCGACTCAACCAGCCCAAAAGGCCGGCCTGGGGGCAGCTCCTTCTC; CAACGGGAAGATCCTCATCAGCGAAAGCACCAACCATGAGACGGCCTTCTCCAAGCTTCCGGGAGACTAT; GCTGACCCCCCAGGGCCTGAGCCAGTGGTCCTAAATGAAGGAAACCAGCGGGTGATCATCAACATTGCTG; GGCTGAGATTTGAGACCCAGCTCAGAACCCTTAGTCAGTTCCCAGAGACTCTCCTGGGAGACCGGGAGAA; AAGGATGCAGTTCTTTGACTCCATGAGAAATGAGTATTTCTTTGATCGTAACCGGCCCAGTTTTGATGGA; ATCCTATATTATTACCAATCTGGTGGGAAAATTCGGCGCCCAGCCAATGTTCCCATTGATATCTTTGCTG; ATGAAATCTCCTTCTATGAGCTGGGTAGTGAGGCCATGGACCAGTTCCGGGAGGATGAAGGCTTCATCAA; AGACCCTGAAACACTGCTACCCACCAATGACATCCACCGTCAGTTCTGGCTCCTCTTTGAGTACCCTGAA; AGTTCCAGCGCTGCCCGTGCTGTGGCCGTGGTCTCGGTGTTGGTTGTGGTCATCTCCATCACCATCTTCT; GCCTGGAGACACTGCCAGAGTTCCGGGAGGATAGGGAGCTGAAGGTGGTCAGAGACCCCAATCTCAACAT; GAGCAAGACAGTCCTCTCCCAGACCATGTTCACCGACCCTTTCTTCATGGTGGAGTCTACCTGCATCGTG; TGGTTCACCTTCGAGCTGGTGCTCCGGTTCGTGGTCTGCCCCAGCAAGACTGACTTCTTCAGGAACATCA; TGAACATCATTGACATCATCTCCATTATCCCCTACTTTGCAACTCTCATCACAGAGCTAGTCCAGGAGAC; AGAGCCGAGTGCCCAACAGAACATGTCCCTGGCCATCCTGAGGATCATCCGCCTGGTGAGGGTCTTCCGC; ATCTTCAAGCTCTCGCGCCACTCCAAGGGGCTGCAGATCCTCGGGCAAACACTGAAGGCGTCCATGCGGG; AGTTGGGGTTGCTCATCTTCTTTCTCTTCATTGGAGTCATCCTCTTCTCCAGTGCAGTCTACTTTGCTGA; GGTGGATGAGCCAGAGTCCCATTTCTCTAGCATTCCTGATGGCTTCTGGTGGGCAGTGGTCACCATGACA; ACTGTAGGCTATGGGGACATGTGCCCGACCACCCCAGGGGGGAAGATTGTGGGCACTCTGTGTGCCATTG; CAGGGGTCCTCACCATTGCCCTCCCTGTGCCTGTCATTGTCTCCAACTTCAATTACTTCTACCACCGGGA; GACTGAGAATGAAGAAAAGCAGAACATCCCGGGAGAAATTGAAAGAATCCTCAACAGTGTAGGCTCAAGA; ATGGGCAGCACAGACTCTCTTAATAAGACCAATGGTGGCTGTTCCACAGAGAAGTCTAGGAAA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210392 representing NM_005549; Red=Cloning site Green=Tags(s); ; MDVCGWKEMEVALVNFDNSDEIQEEPGYATDFDSTSPKGRPGGSSFSNGKILISESTNHETAFSKLPGDY; ADPPGPEPVVLNEGNQRVIINIAGLRFETQLRTLSQFPETLLGDREKRMQFFDSMRNEYFFDRNRPSFDG; ILYYYQSGGKIRRPANVPIDIFADEISFYELGSEAMDQFREDEGFIKDPETLLPTNDIHRQFWLLFEYPE; SSSAARAVAVVSVLVVVISITIFCLETLPEFREDRELKVVRDPNLNMSKTVLSQTMFTDPFFMVESTCIV; WFTFELVLRFVVCPSKTDFFRNIMNIIDIISIIPYFATLITELVQETEPSAQQNMSLAILRIIRLVRVFR; IFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEVDEPESHFSSIPDGFWWAVVTMT; TVGYGDMCPTTPGGKIVGTLCAIAGVLTIALPVPVIVSNFNYFYHRETENEEKQNIPGEIERILNSVGSR; MGSTDSLNKTNGGCSTEKSRK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005549
• ORF Size: 1533 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005549.2, NP_005540.1
• RefSeq Size: 1959 bp
• RefSeq ORF: 1536 bp
• Locus ID: 3744
• UniProt ID: Q16322
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Summary: Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It is specifically regulated by cGMP and postulated to mediate the effects of substances that increase intracellular cGMP. This gene is intronless, and the gene is clustered with genes KCNA2 and KCNA3 on chromosome 1. [provided by RefSeq, Jul 2008]