GJD2 (NM_020660) Human Tagged ORF Clone

SKU RG210158

GJD2 (tGFP-tagged) - Human gap junction protein, delta 2, 36kDa (GJD2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GJD2
• Synonyms: CX36; GJA9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG210158 representing NM_020660; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGGGAATGGACCATCTTGGAGAGGCTGCTAGAAGCCGCGGTGCAGCAGCACTCCACTATGATCGGGA; GGATCCTGTTGACTGTGGTGGTGATCTTCCGGATCCTCATTGTGGCCATTGTGGGGGAGACGGTGTACGA; TGATGAGCAGACCATGTTTGTGTGCAACACCCTGCAGCCCGGCTGTAACCAGGCCTGCTATGACCGCGCC; TTCCCCATCTCCCACATACGTTACTGGGTCTTCCAGATCATAATGGTGTGTACCCCCAGTCTTTGCTTCA; TCACCTACTCTGTGCACCAGTCCGCCAAGCAGCGAGAACGCCGCTACTCTACAGTCTTCCTAGCCCTGGA; CAGAGACCCCCCTGAGTCCATAGGAGGTCCTGGAGGAACTGGGGGTGGGGGCAGTGGTGGGGGCAAACGA; GAAGATAAGAAGTTGCAAAATGCTATTGTGAATGGGGTGCTGCAGAACACAGAGAACACCAGTAAGGAGA; CAGAGCCAGATTGTTTAGAGGTTAAGGAGCTGACTCCACACCCATCAGGTCTACGCACTGCATCAAAATC; CAAGCTCAGAAGGCAGGAAGGCATCTCCCGCTTCTACATTATCCAAGTGGTGTTCCGAAATGCCCTGGAA; ATTGGGTTCCTGGTTGGCCAATATTTTCTCTATGGCTTTAGTGTCCCAGGGTTGTATGAGTGTAACCGCT; ACCCCTGCATCAAGGAGGTGGAATGTTATGTGTCCCGGCCAACTGAGAAGACTGTCTTTCTAGTGTTCAT; GTTTGCTGTAAGTGGCATCTGTGTTGTGCTCAACCTGGCTGAACTCAACCACCTGGGATGGCGCAAGATC; AAGCTGGCTGTGCGAGGGGCTCAGGCCAAGAGAAAGTCAATCTATGAGATTCGTAACAAGGACCTGCCAA; GGGTCAGTGTTCCCAATTTTGGCAGGACTCAGTCCAGTGACTCTGCCTATGTGTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG210158 representing NM_020660; Red=Cloning site Green=Tags(s); ; MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQTMFVCNTLQPGCNQACYDRA; FPISHIRYWVFQIIMVCTPSLCFITYSVHQSAKQRERRYSTVFLALDRDPPESIGGPGGTGGGGSGGGKR; EDKKLQNAIVNGVLQNTENTSKETEPDCLEVKELTPHPSGLRTASKSKLRRQEGISRFYIIQVVFRNALE; IGFLVGQYFLYGFSVPGLYECNRYPCIKEVECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKI; KLAVRGAQAKRKSIYEIRNKDLPRVSVPNFGRTQSSDSAYV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020660
• ORF Size: 965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020660.1, NP_065711.1
• RefSeq Size: 966 bp
• RefSeq ORF: 966 bp
• Locus ID: 57369
• UniProt ID: Q9UKL4
• Cytogenetics: 15q14
• Protein Families: Ion Channels: Other, Transmembrane
• Protein Pathways: Gap junction
• Summary: This gene encodes a member of the connexin protein family. Connexins are gap junction proteins which are arranged in groups of 6 around a central pore to form a connexon, a component of the gap junction intercellular channel. The channels formed by this protein allow cationic molecule exchange between human beta cells and may function in the regulation of insulin secretion. [provided by RefSeq, Oct 2012]