Cezanne (OTUD7B) (NM_020205) Human Tagged ORF Clone

SKU RG209932

OTUD7B (tGFP-tagged) - Human OTU domain containing 7B (OTUD7B)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Cezanne
• Synonyms: CEZANNE; ZA20D1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209932 representing NM_020205; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCCTGGACATGGATGCTGTTCTGTCAGATTTTGTCCGTTCCACAGGAGCAGAGCCAGGGCTAGCGC; GAGATCTCCTAGAAGGAAAGAATTGGGATGTGAATGCCGCCCTCAGTGATTTTGAACAGCTACGTCAAGT; CCATGCTGGAAACCTACCCCCATCCTTTAGTGAGGGGAGTGGTGGCTCCAGGACCCCTGAAAAAGGGTTT; TCTGACAGAGAGCCTACTCGCCCTCCCCGACCCATCCTCCAGCGGCAGGATGACATCGTTCAAGAAAAAC; GCCTGTCTAGGGGCATCTCCCACGCCAGCTCCAGCATTGTTTCCCTGGCCCGGTCCCATGTCTCCTCCAA; TGGTGGGGGTGGGGGGAGCAATGAGCACCCCCTGGAAATGCCCATCTGTGCCTTCCAGCTTCCAGATCTC; ACTGTATACAATGAAGACTTCCGCAGCTTCATAGAGAGAGACCTCATTGAGCAGTCCATGCTGGTTGCCT; TGGAACAGGCAGGGCGTTTGAACTGGTGGGTGAGTGTGGATCCCACCTCTCAGAGGCTGCTTCCTTTGGC; AACTACTGGAGATGGGAACTGCCTCCTGCATGCAGCCTCCCTTGGAATGTGGGGTTTCCATGATCGGGAC; TTGATGCTGCGGAAAGCTTTGTATGCACTGATGGAGAAGGGAGTTGAGAAGGAAGCGTTGAAAAGGCGCT; GGAGGTGGCAGCAGACACAGCAGAATAAAGAGTCAGGGCTGGTATACACAGAAGATGAATGGCAGAAGGA; GTGGAATGAACTGATCAAGCTTGCCTCAAGTGAACCCCGAATGCATCTAGGTACCAATGGAGCCAACTGT; GGTGGGGTGGAGAGTTCTGAGGAGCCTGTATATGAGAGCCTTGAAGAGTTTCACGTCTTTGTCCTTGCTC; ATGTGCTTAGGAGGCCCATAGTCGTCGTGGCAGACACCATGCTGAGGGACTCCGGAGGGGAAGCATTTGC; CCCTATTCCCTTTGGAGGAATCTATCTGCCTTTGGAGGTCCCAGCCAGCCAGTGTCACCGCTCCCCTCTG; GTGCTCGCCTATGATCAGGCCCACTTTTCTGCACTCGTGTCCATGGAGCAGAAGGAGAATACCAAGGAAC; AAGCTGTGATCCCACTTACAGATTCAGAGTATAAGCTGCTGCCCTTGCACTTTGCTGTGGACCCTGGAAA; GGGCTGGGAGTGGGGCAAAGATGATAGTGACAATGTCCGATTGGCCAGTGTAATTCTGTCCCTAGAGGTC; AAATTGCATCTGCTGCATAGCTACATGAATGTGAAGTGGATCCCACTGTCCTCTGATGCACAGGCTCCTC; TGGCCCAGCCTGAGTCCCCCACCGCCTCAGCTGGAGATGAGCCCCGGTCCACTCCTGAGTCTGGAGACTC; AGACAAGGAGTCAGTTGGCAGCAGTTCCACCAGCAACGAGGGCGGCCGGCGGAAGGAGAAGTCAAAGCGA; GATCGGGAGAAGGACAAGAAGAGAGCAGATTCTGTGGCTAACAAACTGGGCAGCTTTGGCAAAACCTTGG; GCAGCAAGCTCAAGAAGAACATGGGGGGCCTGATGCACAGCAAGGGTTCAAAGCCTGGAGGGGTGGGGAC; AGGGTTGGGAGGAAGCAGCGGCACTGAGACACTGGAGAAGAAGAAGAAAAACTCACTGAAGAGCTGGAAG; GGTGGCAAGGAGGAGGCAGCTGGGGATGGGCCTGTGTCTGAGAAGCCCCCAGCTGAGTCTGTTGGTAACG; GAGGGAGCAAGTATAGCCAGGAGGTGATGCAGAGCCTGAGCATTCTGAGGACTGCCATGCAAGGGGAGGG; GAAGTTTATTTTTGTTGGAACCCTGAAGATGGGTCACCGTCACCAGTATCAGGAGGAAATGATCCAGCGC; TACCTTTCTGATGCTGAGGAGAGATTCCTGGCAGAACAGAAGCAGAAGGAGGCAGAGAGGAAGATCATGA; ATGGAGGAATAGGGGGTGGCCCTCCTCCAGCCAAAAAGCCAGAGCCAGATGCTAGGGAAGAGCAGCCGAC; CGGTCCCCCAGCAGAGTCCAGGGCAATGGCATTTTCCACTGGCTACCCTGGGGACTTTACTATCCCTCGG; CCGTCTGGGGGCGGAGTCCACTGCCAGGAACCCCGGAGGCAGTTGGCAGGGGGTCCATGTGTCGGGGGCC; TACCACCATATGCCACCTTCCCCAGACAGTGCCCTCCTGGGCGACCCTACCCCCACCAGGACAGCATCCC; TTCTCTGGAGCCAGGCAGCCACTCTAAGGATGGACTTCACAGGGGTGCCTTGTTACCACCCCCCTACCGA; GTGGCTGATTCCTATAGCAATGGCTACAGAGAGCCCCCTGAGCCAGATGGATGGGCTGGAGGTCTCCGGG; GCCTTCCCCCAACTCAGACCAAATGCAAACAACCGAACTGCAGCTTCTATGGACACCCTGAGACAAACAA; CTTCTGTTCCTGTTGTTACAGGGAAGAACTGAGGAGGAGGGAGCGGGAACCGGATGGGGAGCTCCTGGTG; CACAGGTTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209932 representing NM_020205; Red=Cloning site Green=Tags(s); ; MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGF; SDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDL; TVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRD; LMLRKALYALMEKGVEKEALKRRWRWQQTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANC; GGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPL; VLAYDQAHFSALVSMEQKENTKEQAVIPLTDSEYKLLPLHFAVDPGKGWEWGKDDSDNVRLASVILSLEV; KLHLLHSYMNVKWIPLSSDAQAPLAQPESPTASAGDEPRSTPESGDSDKESVGSSSTSNEGGRRKEKSKR; DREKDKKRADSVANKLGSFGKTLGSKLKKNMGGLMHSKGSKPGGVGTGLGGSSGTETLEKKKKNSLKSWK; GGKEEAAGDGPVSEKPPAESVGNGGSKYSQEVMQSLSILRTAMQGEGKFIFVGTLKMGHRHQYQEEMIQR; YLSDAEERFLAEQKQKEAERKIMNGGIGGGPPPAKKPEPDAREEQPTGPPAESRAMAFSTGYPGDFTIPR; PSGGGVHCQEPRRQLAGGPCVGGLPPYATFPRQCPPGRPYPHQDSIPSLEPGSHSKDGLHRGALLPPPYR; VADSYSNGYREPPEPDGWAGGLRGLPPTQTKCKQPNCSFYGHPETNNFCSCCYREELRRREREPDGELLV; HRF; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020205
• ORF Size: 2529 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020205.4
• RefSeq Size: 6413 bp
• RefSeq ORF: 2532 bp
• Locus ID: 56957
• UniProt ID: Q6GQQ9
• Cytogenetics: 1q21.2
• Summary: Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B. Negatively regulates mucosal immunity against infections (By similarity). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (By similarity). Mediates deubiquitination of EGFR (PubMed:22179831). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains (PubMed:27732584). Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure (PubMed:27732584). Hydrolyzes both linear and branched forms of polyubiquitin.[UniProtKB/Swiss-Prot Function]