DNA Ligase III (LIG3) (NM_013975) Human Tagged ORF Clone

SKU RG209530

LIG3 (tGFP-tagged) - Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DNA Ligase III
• Synonyms: LIG2; LIG3alpha
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209530 representing NM_013975; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCCTAT; TCCGAAAACATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACA; TCCCCTCTTCCTGAGAAGAAAGCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTAC; CTTGTTTTCTTGCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCTGAGCAACGGTTCT; GTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAAGGGCGTATG; CCGAATTGGCAAAGTGGTGCCCAATCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATT; AAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCACAAAAAAAATCGAGGACCTCACAGAGCTGG; AAGGCTGGGAAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTAA; GGCAGCAGGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCCAGGTGACTTCT; CCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATCCCCGGAAATTTTCTGGCTTTTCAGCCAAGCCCA; ACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGAGAAGTCTGTCTTCAAGCAAATGTGACCCCAG; GCATAAGGACTGTCTGCTACGGGAGTTTCGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAAC; ACGAAGACCCAGATCATCCAGGACTTCCTTCGGAAAGGCTCAGCAGGAGATGGTTTCCACGGTGATGTGT; ACCTAACAGTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACGATAAGCAGATTGT; GAAGCTTTTCAGTCGCATTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAGCAGGGTGACGTG; TCAGAGACAATCAGAGTCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAGAGCCTCCTTACCA; TCCAGGAAGTGGATGAGTTCCTTCTGCGGCTGTCCAAGCTCACCAAGGAGGATGAGCAGCAACAGGCCCT; ACAGGACATTGCCTCCAGGTGTACAGCCAATGACCTTAAATGCATCATCAGGTTGATCAAACATGATCTG; AAGATGAACTCAGGTGCAAAACATGTGTTAGACGCCCTTGACCCCAATGCCTATGAAGCCTTCAAAGCCT; CGCGCAACCTGCAGGATGTGGTGGAGCGGGTCCTTCACAACGCGCAGGAGGTGGAGAAGGAGCCGGGCCA; GAGACGAGCTCTGAGCGTCCAGGCCTCGCTGATGACACCTGTGCAGCCCATGTTGGCGGAGGCCTGCAAG; TCCGTTGAGTATGCAATGAAGAAATGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGAGAGCGAG; TCCAGGTGCATAAGAATGGAGACCACTTCAGCTACTTCAGCCGCAGTCTCAAGCCCGTCCTTCCTCACAA; GGTGGCCCACTTTAAGGACTACATTCCCCAGGCTTTTCCTGGGGGCCACAGCATGATCTTGGATTCTGAA; GTGCTTCTGATTGACAACAAGACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTACACAAGAAAGCAG; CCTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGATGGA; CAGACCTCTGTGTGAGCGGCGGAAGTTTCTTCATGACAACATGGTTGAAATTCCAAACCGGATCATGTTC; TCAGAAATGAAGCGAGTCACAAAAGCTTTGGACTTGGCTGACATGATAACCCGGGTGATCCAGGAGGGAT; TGGAGGGGCTGGTGCTGAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAAAGTGAA; GAAAGACTATTTGAACGAGGGGGCCATGGCCGACACAGCTGACCTGGTGGTCCTTGGAGCCTTCTATGGG; CAAGGGAGCAAAGGCGGCATGATGTCAATCTTCCTCATGGGCTGCTACGACCCTGGCAGCCAGAAGTGGT; GCACAGTCACCAAGTGTGCAGGAGGCCATGATGATGCCACGCTTGCCCGCCTGCAGAATGAACTAGACAT; GGTGAAGATCAGCAAGGACCCCAGCAAAATACCCAGCTGGTTGAAGGTCAACAAGATCTACTATCCTGAC; TTCATCGTCCCAGACCCAAAGAAAGCTGCCGTGTGGGAGATCACAGGGGCTGAATTCTCCAAATCGGAGG; CTCATACAGCTGACGGGATCTCCATCCGATTCCCTCGCTGCACCCGAATCCGAGATGATAAGGACTGGAA; ATCTGCCACTAACCTTCCCCAACTCAAGGAACTGTACCAGTTGTCCAAGGAGAAGGCAGACTTCACTGTA; GTGGCTGGAGATGAGGGGAGCTCCACTACAGGGGGTAGCAGTGAAGAGAATAAGGGTCCCTCAGGGTCTG; CTGTGTCCCGCAAGGCCCCCAGCAAGCCCTCAGCCAGTACCAAGAAAGCAGAAGGGAAGCTGAGTAACTC; CAACAGCAAAGATGGCAACATGCAGACTGCAAAGCCTTCCGCTATGAAGGTGGGGGAGAAGCTGGCCACA; AAGTCTTCTCCAGTGAAAGTAGGGGAGAAGCGGAAAGCTGCTGATGAGACGCTGTGCCAAACAAAGGTAT; TGCTGGACATCTTCACTGGGGTGCGGCTTTACTTGCCACCCTCCACACCAGACTTCAGCCGTCTCAGACG; CTACTTTGTGGCATTCGACGGGGACCTGGTACAGGAATTTGATATGACTTCAGCCACGCACGTGCTGGGT; AGCAGGGACAAGAACCCTGCGGCCCAGCAGGTCTCCCCAGAGTGGATTTGGGCATGTATCCGGAAACGGA; GACTGGTAGCTCCCTGC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209530 representing NM_013975; Red=Cloning site Green=Tags(s); ; MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATY; LVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHI; KCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTS; PVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYN; TKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDV; SETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDL; KMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACK; SVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSE; VLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMF; SEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYG; QGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPD; FIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTV; VAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLAT; KSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLG; SRDKNPAAQQVSPEWIWACIRKRRLVAPC; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013975
• ORF Size: 3027 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013975.4
• RefSeq Size: 3701 bp
• RefSeq ORF: 3030 bp
• Locus ID: 3980
• UniProt ID: P49916
• Cytogenetics: 17q12
• Domains: BRCT, DNA_ligase, zf-PARP
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Summary: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]