VPS11 (NM_021729) Human Tagged ORF Clone

SKU RG209474

VPS11 (tGFP-tagged) - Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: VPS11
• Synonyms: END1; HLD12; hVPS11; PEP5; RNF108
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209474 representing NM_021729; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCCTACCTGCAGTGGCGGCGCTTCGTTTTCTTCGACAAGGAGCTGGTGAAGGAGCCGCTGAGCA; ATGATGGGGCCGCTCCCGGGGCCACACCTGCTTCTGGATCCGCTGCTTCCAAGTTCCTTTGCCTCCCTCC; TGGCATCACTGTCTGCGACTCAGGCCGAGGGAGCCTGGTCTTTGGAGATATGGAAGGCCAGATCTGGTTC; TTGCCACGTTCCCTACAGCTTACAGGCTTCCAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGA; AGCAGCACAATATTCTGGCATCTGTTGGAGAAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAA; CCTGGAGAAGAGAGATGGTGGCAATCCACTCTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCA; ACTGTTGTATCTTGTTTGACTGTCCATGAAAATCTCAACTTTATGGCCATTGGTTTCACAGATGGGAGTG; TTACATTGAACAAAGGAGACATCACCCGGGACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTA; TCCTGTAACTGGATTGGCCTTTCGCCAAGCAGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAAC; GTCCAGTCCTATATAGTTTCTGGAAAAGACTACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGC; GCTGCTCAGCCCTAAGTGACCCTTCTCAGGACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTT; GTACCAGCCTGATGAACGTGGGCCCTGCTTCGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGA; GGCTACCTTATCATTGTCTCCCGTGACCGGAAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCAC; AGAGCTCCGACAAGCAGATTCTAAACATCTATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTT; TGAGGATGTAGTGGATGTGCTTGCTGAGTGGGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCAC; GCACTGCAGGAGAAGGACACACAGACCAAACTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGA; TTAACCTTGCCAAGAGCCAGCATCTGGACAGTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCA; TCTCTACAGCAAGGGCAACCACGATGGGGCTGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCA; TCCTACGTGATCCGCAAGTTTCTGGATGCCCAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGC; ACCGACAATCCCTGGCCAATGCCGACCATACCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAG; CTCGAAGCTGGAGGAGTTCATCAAGAAAAAGAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATC; AAGGTCCTCCGGCAGGCTGGCTACTACTCCCATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGT; GGTACCTGAAGATCCAGCTAGAAGACATTAAGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCC; TTTTGAGCAGGCAGAGAGCAACATGAAGCGCTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACA; ACTCAGTTGCTGAAGGGACTTTGTACTGATTATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCC; CAGGCTGCAGGGCCAACTCTGAGGAGTTCATCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTT; CCTAGAGCACATGAGTGAAGTGCAGCCAGACTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGA; CTGCAGAACTGGGCCCACGAGAAGGATCCACAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGC; TGAAGAGTGGTCGCTTCTGTGACGTCTTTGACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGA; TGGTGTCCTTTACCTTTATGAGCAGGGGAAGCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAG; CAGTACCGGCAGGTCATCAGCGTGTGTGAGCGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCCC; TCAGCTACTTCGCTCGCAAGGAGGAGGACTGCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAGAA; CAAGAACCTCATGCCACCTCTTCTAGTGGTGCAGACCCTGGCCCACAACTCCACAGCCACACTCTCCGTC; ATCAGGGACTACCTGGTCCAAAAACTACAGAAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGGGTGC; GGCGGTACCGAGAGGAGACCACCCGTATCCGCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAGATTTT; CCAAAAGACCAAGTGCAGCATCTGTAACAGTGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGTGGCCAC; TCCTTCCACCAACACTGCTTTGAGAGTTACTCGGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAAA; ACCGGAAGGTCATGGATATGATCCGGGCCCAGGAACAGAAACGAGATCTCCATGATCAATTCCAGCATCA; GCTCAGGTGCTCCAATGACAGCTTTTCTGTGATTGCTGACTACTTTGGCAGAGGTGTTTTCAACAAATTG; ACTCTGCTGACCGACCCTCCCACAGCCAGACTGACCTCCAGCCTGGAGGCTGGGCTGCAACGCGACCTAC; TCATGCACTCCAGGAGGGGCACT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209474 representing NM_021729; Red=Cloning site Green=Tags(s); ; MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWF; LPRSLQLTGFQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEP; TVVSCLTVHENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTEN; VQSYIVSGKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFR; GYLIIVSRDRKVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVH; ALQEKDTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEP; SYVIRKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAI; KVLRQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQT; TQLLKGLCTDYRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELR; LQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHE; QYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSV; IRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGH; SFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLRCSNDSFSVIADYFGRGVFNKL; TLLTDPPTARLTSSLEAGLQRDLLMHSRRGT; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021729
• ORF Size: 2823 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021729.4, NP_068375.3
• RefSeq Size: 3279 bp
• RefSeq ORF: 2826 bp
• Locus ID: 55823
• UniProt ID: Q9H270
• Cytogenetics: 11q23.3
• Domains: Clathrin, RING
• Protein Families: Druggable Genome
• Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]