AGO1 (NM_012199) Human Tagged ORF Clone

SKU RG209163

AGO1 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 1 (EIF2C1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: AGO1
• Synonyms: EIF2C; EIF2C1; GERP95; hAgo1; Q99
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209163 representing NM_012199; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAAGCGGGACCCTCGGGAGCAGCTGCGGGCGCTTACCTGCCCCCCCTGCAGCAGGTGTTCCAGGCAC; CTCGCCGGCCTGGCATTGGCACTGTGGGGAAACCAATCAAGCTCCTGGCCAATTACTTTGAGGTGGACAT; CCCTAAGATCGACGTGTACCACTACGAGGTGGACATCAAGCCGGATAAGTGTCCCCGTAGAGTCAACCGG; GAAGTGGTGGAATACATGGTCCAGCATTTCAAGCCTCAGATCTTTGGTGATCGCAAGCCTGTGTATGATG; GAAAGAAGAACATTTACACTGTCACAGCACTGCCCATTGGCAACGAACGGGTCGACTTTGAGGTGACAAT; CCCTGGGGAAGGGAAGGATCGAATCTTTAAGGTCTCCATCAAGTGGCTAGCCATTGTGAGCTGGCGAATG; CTGCATGAGGCCCTGGTCAGCGGCCAGATCCCTGTTCCCTTGGAGTCTGTGCAAGCCCTGGATGTGGCCA; TGAGGCACCTGGCATCCATGAGGTACACCCCTGTGGGCCGCTCCTTCTTCTCACCGCCTGAGGGCTACTA; CCACCCGCTGGGGGGTGGGCGCGAGGTCTGGTTCGGCTTTCACCAGTCTGTGCGCCCTGCCATGTGGAAG; ATGATGCTCAACATTGATGTCTCAGCCACTGCCTTTTATAAGGCACAGCCAGTGATTGAGTTCATGTGTG; AGGTGCTGGACATCAGGAACATAAATGAGCAGCCCAAGCCCCTCACGGACTCTCAGCGCGTTCGCTTCAC; CAAGGAGATCAAGGGCCTGAAGGTGGAAGTCACCCACTGTGGACAGATGAAGAGGAAGTACCGCGTGTGT; AATGTTACCCGTCGCCCTGCTAGCCATCAGACATTCCCCTTACAGCTGGAGAGTGGACAGACTGTGGAGT; GCACAGTGGCACAGTATTTCAAGCAGAAATATAACCTTCAGCTCAAGTATCCCCATCTGCCCTGCCTACA; AGTTGGCCAGGAACAAAAGCATACCTACCTTCCCCTAGAGGTCTGTAACATTGTGGCTGGGCAGCGCTGT; ATTAAAAAGCTGACCGACAACCAGACCTCGACCATGATAAAGGCCACAGCTAGATCCGCTCCAGACAGAC; AGGAGGAGATCAGTCGCCTGATGAAGAATGCCAGCTACAACTTAGATCCCTACATCCAGGAATTTGGGAT; CAAAGTGAAGGATGACATGACGGAGGTGACAGGGCGAGTGCTGCCGGCGCCCATCTTGCAGTACGGCGGC; CGGAACCGGGCCATTGCCACACCCAATCAGGGTGTCTGGGACATGCGGGGGAAACAGTTCTACAATGGGA; TTGAGATCAAAGTCTGGGCCATCGCCTGCTTCGCACCCCAAAAACAGTGTCGAGAAGAGGTGCTCAAGAA; CTTCACAGACCAGCTGCGGAAGATTTCCAAGGATGCGGGGATGCCTATCCAGGGTCAACCTTGTTTCTGC; AAATATGCACAGGGGGCAGACAGCGTGGAGCCTATGTTCCGGCATCTCAAGAACACCTACTCAGGGCTGC; AGCTCATTATTGTCATCCTGCCAGGGAAGACGCCGGTGTATGCTGAGGTGAAACGTGTCGGAGATACACT; CTTGGGAATGGCTACGCAGTGTGTGCAGGTGAAGAACGTGGTCAAGACCTCACCTCAGACTCTGTCCAAC; CTCTGCCTCAAGATCAATGTCAAACTTGGTGGCATTAACAACATCCTAGTCCCACACCAGCGCTCTGCCG; TTTTTCAACAGCCAGTGATATTCCTGGGAGCAGATGTTACACACCCCCCAGCAGGGGATGGGAAAAAACC; TTCTATCACAGCAGTGGTAGGCAGTATGGATGCCCACCCCAGCCGATACTGTGCTACTGTGCGGGTACAG; CGACCACGGCAAGAGATCATTGAAGACTTGTCCTACATGGTGCGTGAGCTCCTCATCCAATTCTACAAGT; CCACCCGTTTCAAGCCTACCCGCATCATCTTCTACCGAGATGGGGTGCCTGAAGGCCAGCTACCCCAGAT; ACTCCACTATGAGCTACTGGCCATTCGTGATGCCTGCATCAAACTGGAAAAGGACTACCAGCCTGGGATC; ACTTATATTGTGGTGCAGAAACGCCATCACACCCGCCTTTTCTGTGCTGACAAGAATGAGCGAATTGGGA; AGAGTGGTAACATCCCAGCTGGGACCACAGTGGACACCAACATCACCCACCCATTTGAGTTTGACTTCTA; TCTGTGCAGCCACGCAGGCATCCAGGGCACCAGCCGACCATCCCATTACTATGTTCTTTGGGATGACAAC; CGTTTCACAGCAGATGAGCTCCAGATCCTGACGTACCAGCTGTGCCACACTTACGTACGATGCACACGCT; CTGTCTCTATCCCAGCACCTGCCTACTATGCCCGCCTGGTGGCTTTCCGGGCACGATACCACCTGGTGGA; CAAGGAGCATGACAGTGGAGAGGGGAGCCACATATCGGGGCAGAGCAATGGGCGGGACCCCCAGGCCCTG; GCCAAAGCCGTGCAGGTTCACCAGGATACTCTGCGCACCATGTACTTCGCT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209163 representing NM_012199; Red=Cloning site Green=Tags(s); ; MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNR; EVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRM; LHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWK; MMLNIDVSATAFYKAQPVIEFMCEVLDIRNINEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVC; NVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRC; IKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGG; RNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFC; KYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSN; LCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQ; RPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGI; TYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDN; RFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQAL; AKAVQVHQDTLRTMYFA; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012199
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012199.2, NP_036331.1
• RefSeq Size: 7478 bp
• RefSeq ORF: 2574 bp
• Locus ID: 26523
• UniProt ID: Q9UL18
• Cytogenetics: 1p34.3
• Protein Families: Druggable Genome
• Summary: This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]