GPT2 (NM_133443) Human Tagged ORF Clone

SKU RG209119

GPT2 (tGFP-tagged) - Human glutamic pyruvate transaminase (alanine aminotransferase) 2 (GPT2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GPT2
• Synonyms: ALT2; GPT 2; MRT49; NEDSPM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209119 representing NM_133443; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCAGCGGGCGGCGGCGCTGGTCCGGCGGGGCTGTGGTCCCCGGACCCCCAGCTCCTGGGGCCGCAGCC; AGAGCAGCGCGGCCGCCGAGGCCTCGGCGGTGCTCAAGGTGCGGCCCGAGCGCAGCCGGCGCGAGCGCAT; CCTCACGCTGGAGTCCATGAACCCGCAGGTGAAGGCGGTGGAGTACGCCGTGCGGGGACCCATCGTGCTC; AAGGCCGGCGAGATCGAGCTCGAGCTGCAGCGGGGTATCAAAAAGCCATTCACAGAGGTCATCCGAGCCA; ACATCGGGGACGCCCAGGCTATGGGGCAGCAGCCAATCACCTTCCTCCGGCAGGTGATGGCACTATGCAC; CTACCCAAACCTGCTGGACAGCCCCAGCTTCCCAGAAGATGCTAAGAAACGTGCCCGGCGGATCCTGCAG; GCTTGTGGCGGGAACAGCCTGGGGTCCTACAGTGCTAGCCAGGGTGTCAACTGCATCCGTGAAGATGTGG; CTGCCTACATCACCAGGAGGGATGGCGGTGTGCCTGCGGACCCCGACAACATCTACCTGACCACGGGAGC; TAGTGACGGCATTTCTACGATCCTGAAGATCCTCGTCTCCGGGGGCGGCAAGTCACGGACAGGTGTGATG; ATCCCCATCCCACAATATCCCCTCTATTCAGCTGTCATCTCTGAGCTCGACGCCATCCAGGTGAATTACT; ACCTGGACGAGGAGAACTGCTGGGCGCTGAATGTGAATGAGCTCCGGCGGGCGGTGCAGGAGGCCAAAGA; CCACTGTGATCCTAAGGTGCTCTGCATAATCAACCCTGGGAACCCCACAGGCCAGGTACAAAGCAGAAAG; TGCATAGAAGATGTGATCCACTTTGCCTGGGAAGAGAAGCTCTTTCTCCTGGCTGATGAGGTGTACCAGG; ACAACGTGTACTCTCCAGATTGCAGATTCCACTCCTTCAAGAAGGTGCTGTACGAGATGGGGCCCGAGTA; CTCCAGCAACGTGGAGCTCGCCTCCTTCCACTCCACCTCCAAGGGCTACATGGGCGAGTGTGGTTACAGA; GGAGGCTACATGGAGGTGATCAACCTGCACCCTGAGATCAAGGGCCAGCTGGTGAAGCTGCTGTCGGTGC; GCCTGTGCCCCCCAGTGTCTGGGCAGGCCGCCATGGACATTGTCGTGAACCCCCCGGTGGCAGGAGAGGA; GTCCTTTGAGCAATTCAGCCGAGAGAAGGAGTCGGTCCTGGGTAATCTGGCCAAAAAAGCAAAGCTGACG; GAAGACCTGTTTAACCAAGTCCCAGGAATTCACTGCAACCCCTTGCAGGGGGCCATGTACGCCTTCCCTC; GGATCTTCATTCCTGCCAAAGCTGTGGAGGCTGCTCAGGCCCATCAAATGGCTCCAGACATGTTCTACTG; CATGAAGCTCCTGGAGGAGACTGGCATCTGTGTCGTGCCCGGCAGTGGCTTTGGGCAGAGGGAAGGCACT; TACCACTTCAGGATGACTATCCTCCCTCCAGTGGAGAAGCTGAAAACGGTGCTGCAGAAGGTGAAAGACT; TCCACATCAACTTCCTGGAGAAGTACGCG; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209119 representing NM_133443; Red=Cloning site Green=Tags(s); ; MQRAAALVRRGCGPRTPSSWGRSQSSAAAEASAVLKVRPERSRRERILTLESMNPQVKAVEYAVRGPIVL; KAGEIELELQRGIKKPFTEVIRANIGDAQAMGQQPITFLRQVMALCTYPNLLDSPSFPEDAKKRARRILQ; ACGGNSLGSYSASQGVNCIREDVAAYITRRDGGVPADPDNIYLTTGASDGISTILKILVSGGGKSRTGVM; IPIPQYPLYSAVISELDAIQVNYYLDEENCWALNVNELRRAVQEAKDHCDPKVLCIINPGNPTGQVQSRK; CIEDVIHFAWEEKLFLLADEVYQDNVYSPDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYR; GGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLT; EDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQAHQMAPDMFYCMKLLEETGICVVPGSGFGQREGT; YHFRMTILPPVEKLKTVLQKVKDFHINFLEKYA; ; SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133443
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_133443.4
• RefSeq Size: 3957 bp
• RefSeq ORF: 1572 bp
• Locus ID: 84706
• UniProt ID: Q8TD30
• Cytogenetics: 16q11.2
• Domains: aminotran_1_2
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Metabolic pathways
• Summary: This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]