hnRNP L (HNRNPL) (NM_001005335) Human Tagged ORF Clone

SKU RG209067

HNRNPL (tGFP-tagged) - Human heterogeneous nuclear ribonucleoprotein L (HNRNPL), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: hnRNP L
• Synonyms: hnRNP-L; HNRPL; P/OKcl.14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209067 representing NM_001005335; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGAAGATGGCGGCGGCGGGCGGCGGAGGCGGCGGTGGCCGCTACTACGGCGGCGGCAGTGAGGGCG; GCCGGGCCCCTAAGCGGCTCAAGACTGACAACGCCGGCGACCAGCACGGAGGCGGCGGCGGTGGCGGTGG; AGGAGCCGGGGCGGCGGGCGGCGGCGGCGGTGGGGAGAACTACGATGACCCGCACAAAACCCCTGCCTCC; CCAGTTGTCCACATCAGGGGCCTGATTGACGGTGTGGTGGAAGCAGACCTTGTGGAGGCCTTGCAGGAGT; TTGGACCCATCAGCTATGTGGTGGTAATGCCTAAAAAGAGACAAGCACTGGTGGAGTTTGAAGATGTGTT; GGGGGCTTGCAACGCAGTGAACTACGCAGCCGACAACCAAATATACATTGCTGGTCACCCAGCTTTTGTC; AACTACTCTACCAGCCAGAAGATCTCCCGCCCTGGGGACTCGGATGACTCCCGGAGCGTGAACAGTGTGC; TTCTCTTTACCATCCTGAACCCCATTTATTCGATCACCACGGATGTTCTTTACACTATCTGTAATCCTTG; TGGCCCTGTCCAGAGAATTGTCATTTTCAGGAAGAATGGAGTTCAGGCGATGGTGGAATTTGACTCAGTT; CAAAGTGCCCAGCGGGCCAAGGCCTCTCTCAATGGGGCTGATATCTATTCTGGCTGTTGCACTCTGAAGA; TCGAATACGCAAAGCCTACACGCTTGAATGTGTTCAAGAATGATCAGGATACTTGGGACTACACAAACCC; CAATCTCAGTGGACAAGGTGACCCTGGCAGCAACCCCAACAAACGCCAGAGGCAGCCCCCTCTCCTGGGA; GATCACCCCGCAGAATATGGAGGGCCCCACGGTGGGTACCACAGCCATTACCATGATGAGGGCTACGGGC; CCCCCCCACCTCACTACGAAGGGAGAAGGATGGGTCCACCAGTGGGGGGTCACCGTCGGGGCCCAAGTCG; CTACGGCCCCCAGTATGGGCACCCCCCACCCCCTCCCCCACCACCCGAGTATGGCCCTCACGCCGACAGC; CCTGTGCTCATGGTCTATGGCTTGGATCAATCTAAGATGAACTGTGACCGAGTCTTCAATGTCTTCTGCT; TATATGGCAATGTGGAGAAGGTGAAATTCATGAAAAGCAAGCCGGGGGCCGCCATGGTGGAGATGGCTGA; TGGCTACGCTGTAGACCGGGCCATTACCCACCTCAACAACAACTTCATGTTTGGGCAGAAGCTGAATGTC; TGTGTCTCCAAGCAGCCAGCCATCATGCCTGGTCAGTCATACGGGTTGGAAGACGGGTCTTGCAGTTACA; AAGACTTCAGTGAATCCCGGAACAATCGGTTCTCCACCCCAGAGCAGGCAGCCAAGAACCGCATCCAGCA; CCCCAGCAACGTGCTGCACTTCTTCAACGCCCCGCTGGAGGTGACCGAGGAGAACTTCTTTGAGATCTGC; GATGAGCTGGGAGTGAAGCGGCCATCTTCTGTGAAAGTATTCTCAGGCAAAAGTGAGCGCAGCTCCTCTG; GACTGCTGGAGTGGGAATCCAAGAGCGATGCCCTGGAGACTCTGGGCTTCCTGAACCATTACCAGATGAA; AAACCCAAATGGTCCATACCCTTACACTCTGAAGTTGTGTTTCTCCACTGCTCAGCACGCCTCC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209067 representing NM_001005335; Red=Cloning site Green=Tags(s); ; MVKMAAAGGGGGGGRYYGGGSEGGRAPKRLKTDNAGDQHGGGGGGGGGAGAAGGGGGGENYDDPHKTPAS; PVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFV; NYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSV; QSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLG; DHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADS; PVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNV; CVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEIC; DELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005335
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001005335.1, NP_001005335.1
• RefSeq Size: 1895 bp
• RefSeq ORF: 1371 bp
• Locus ID: 3191
• UniProt ID: P14866
• Cytogenetics: 19q13.2
• Summary: Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]