POU6F1 (NM_002702) Human Tagged ORF Clone

SKU
RG208598
POU6F1 (tGFP-tagged) - Human POU class 6 homeobox 1 (POU6F1), transcript variant 1
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    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $500.00 MSRP $500.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol POU6F1
Synonyms BRN5; MPOU; TCFB1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG208598 representing NM_002702
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGGGATCAGCAGTCAGATCCTCACCAATGCTCAGGGACAGGTTATTGGAACCCTTCCATGGGTAG
TGAACTCAGCTAGTGTGGCGGCCCCAGCACCAGCCCAAAGCCTGCAGGTCCAGGCCGTGACCCCCCAGCT
GTTGTTGAACGCCCAGGGCCAGGTGATTGCGACCCTGGCTAGCAGCCCCCTGCCTCCACCTGTGGCTGTC
CGGAAGCCAAGCACACCTGAGTCCCCTGCTAAGAGTGAGGTGCAGCCCATCCAGCCCACACCAACCGTGC
CCCAGCCTGCTGTGGTCATTGCCAGCCCAGCTCCAGCCGCCAAGCCATCTGCCTCTGCTCCTATCCCAAT
TACCTGCTCAGAGACCCCCACCGTCAGCCAGTTGGTGTCCAAGCCACATACTCCAAGTCTGGATGAGGAT
GGGATCAACTTAGAAGAGATCCGGGAGTTTGCCAAGAACTTTAAGATCCGGCGGCTCTCGCTGGGCCTTA
CACAGACCCAGGTGGGTCAGGCTCTGACTGCAACGGAAGGTCCAGCCTACAGCCAGTCAGCCATCTGCCG
GTTCGAGAAGCTAGACATCACACCCAAGAGTGCCCAGAAGCTAAAGCCGGTGCTGGAAAAGTGGCTAAAC
GAAGCTGAACTGCGGAACCAGGAAGGCCAGCAGAACCTGATGGAGTTTGTGGGAGGCGAGCCCTCCAAGA
AACGCAAACGCCGCACCTCCTTCACCCCCCAGGCCATAGAGGCTCTCAATGCCTATTTTGAGAAGAACCC
ACTGCCCACAGGCCAGGAGATCACTGAAATTGCTAAGGAGCTCAACTACGACCGTGAGGTAGTGCGGGTC
TGGTTCTGCAATCGGCGCCAGACGCTCAAGAACACCAGCAAGCTGAACGTCTTTCAGATCCCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG208598 representing NM_002702
Red=Cloning site Green=Tags(s)

MPGISSQILTNAQGQVIGTLPWVVNSASVAAPAPAQSLQVQAVTPQLLLNAQGQVIATLASSPLPPPVAV
RKPSTPESPAKSEVQPIQPTPTVPQPAVVIASPAPAAKPSASAPIPITCSETPTVSQLVSKPHTPSLDED
GINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLEKWLN
EAELRNQEGQQNLMEFVGGEPSKKRKRRTSFTPQAIEALNAYFEKNPLPTGQEITEIAKELNYDREVVRV
WFCNRRQTLKNTSKLNVFQIP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_002702
ORF Size 903 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002702.3
RefSeq Size 2911 bp
RefSeq ORF 906 bp
Locus ID 5463
UniProt ID Q14863
Cytogenetics 12q13.13
Domains homeobox, POU
Protein Families Transcription Factors
Summary Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').[UniProtKB/Swiss-Prot Function]
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Citations

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