Vesicle docking protein p115 (USO1) (NM_003715) Human Tagged ORF Clone

SKU RG207614

USO1 (tGFP-tagged) - Human USO1 vesicle docking protein homolog (yeast) (USO1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Vesicle docking protein p115
• Synonyms: P115; TAP; VDP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207614 representing NM_003715; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATTTCCTCCGCGGGGTAATGGGGGGTCAGAGTGCCGGACCCCAGCACACAGAAGCCGAGACGATTC; AAAAGCTTTGTGACAGAGTAGCTTCATCTACTTTATTGGATGATCGAAGAAATGCTGTTCGTGCTCTCAA; ATCATTATCTAAGAAATACCGCTTGGAAGTGGGTATACAAGCTATGGAACATCTTATTCATGTTTTACAA; ACAGATCGTTCAGATTCTGAAATAATAGGTTATGCTTTGGACACACTATATAATATAATATCTAATGAAG; AAGAAGAAGTAGAAGAAAATTCCACAAGACAGAGTGAAGATTTGGGAAGCCAATTTACAGAAATTTTCAT; TAAGCAGCAGGAAAATGTCACTCTTCTGTTATCTTTATTGGAGGAGTTTGATTTCCATGTCCGCTGGCCT; GGTGTGAAGCTTCTTACTTCTCTTTTAAAACAACTAGGGCCTCAGGTGCAACAAATTATTTTAGTCAGTC; CTATGGGTGTTTCAAGATTGATGGACTTACTAGCGGATTCCAGGGAAGTTATACGTAATGATGGCGTCTT; ACTACTGCAGGCACTAACAAGAAGCAATGGTGCAATCCAGAAAATTGTTGCTTTTGAAAATGCTTTCGAG; AGACTACTGGACATTATTTCAGAGGAGGGGAACAGTGATGGAGGTATAGTAGTTGAAGATTGTTTGATTT; TGCTCCAAAACTTATTAAAAAACAACAACTCCAATCAAAATTTTTTTAAAGAAGGCTCATATATTCAACG; TATGAAACCTTGGTTTGAAGTTGGAGATGAAAATTCTGGCTGGTCTGCACAGAAAGTGACCAATCTACAT; CTAATGCTACAGCTTGTTCGTGTATTGGTATCTCCCACCAACCCTCCTGGTGCTACCAGTAGCTGCCAGA; AGGCTATGTTCCAGTGTGGGTTATTGCAGCAGCTTTGTACTATCCTAATGGCTACTGGGGTTCCTGCTGA; TATCCTGACTGAGACCATAAATACTGTATCAGAAGTTATTCGAGGCTGCCAAGTAAACCAAGACTACTTT; GCATCTGTAAATGCACCTTCAAACCCACCAAGACCGGCAATTGTAGTACTTCTCATGTCCATGGTTAATG; AAAGGCAGCCATTTGTTTTGCGCTGTGCTGTTCTCTATTGTTTCCAGTGTTTCTTGTATAAAAACCAAAA; AGGACAAGGAGAAATCGTGTCAACACTTTTACCTTCTACCATTGATGCAACAGGTAATTCAGTTTCAGCT; GGCCAGTTATTATGTGGAGGTTTGTTTTCTACTGATTCACTTTCAAACTGGTGTGCTGCTGTGGCCCTTG; CCCATGCGTTGCAAGAAAATGCCACCCAGAAAGAACAGTTGCTCAGGGTTCAACTTGCTACAAGTATTGG; CAACCCTCCAGTTTCTTTACTTCAACAGTGCACCAATATTCTTTCACAGGGAAGCAAAATACAAACAAGA; GTTGGATTATTAATGTTGCTTTGTACCTGGCTAAGCAATTGTCCCATTGCAGTAACGCATTTTCTTCACA; ATTCAGCCAATGTTCCATTCCTTACAGGACAAATTGCAGAAAATCTTGGAGAAGAAGAGCAGTTGGTCCA; AGGCTTATGTGCCCTTTTGTTGGGCATTTCGATTTATTTCAATGATAACTCACTTGAGAGCTACATGAAA; GAGAAGCTAAAACAACTGATTGAGAAGAGGATTGGCAAAGAGAATTTCATAGAGAAACTAGGATTTATTA; GCAAACATGAGTTGTATTCCAGAGCATCTCAGAAACCCCAGCCAAACTTTCCCAGTCCAGAATACATGAT; ATTTGATCATGAGTTTACGAAGCTGGTAAAAGAACTTGAAGGTGTTATAACTAAGGCTATTTATAAGTCC; AGTGAAGAAGATAAAAAAGAAGAAGAGGTGAAAAAAACATTAGAACAGCATGACAATATTGTGACTCACT; ACAAAAATATGATTCGAGAGCAAGATCTCCAACTTGAGGAATTAAGGCAGCAGGTTTCTACATTAAAATG; TCAAAATGAACAGCTCCAGACGGCAGTCACACAGCAAGTATCACAGATCCAGCAGCACAAAGACCAGTAT; AATCTTCTTAAAATACAGCTAGGAAAAGACAATCAGCATCAAGGTTCTTACAGTGAGGGGGCTCAGATGA; ATGGCATTCAGCCAGAAGAAATTGGTAGATTGCGAGAAGAGATAGAAGAATTAAAACGTAATCAGGAACT; TTTACAAAGCCAGCTGACTGAAAAGGACTCTATGATTGAAAATATGAAATCTTCCCAAACATCTGGCACA; AATGAACAGTCTTCAGCAATAGTTTCAGCTAGAGATTCTGAACAAGTTGCAGAATTAAAACAGGAACTGG; CAACTTTAAAGTCTCAGTTAAACTCACAATCTGTGGAGATCACCAAACTACAGACAGAAAAGCAGGAACT; GTTACAGAAAACAGAAGCGTTTGCAAAATCAGTTGAGGTACAAGGAGAGACCGAGACTATAATAGCCACC; AAAACTACTGATGTAGAAGGAAGACTGTCAGCATTATTACAAGAGACCAAAGAGTTAAAGAATGAAATTA; AAGCTCTGTCTGAGGAAAGAACTGCCATTAAAGAGCAGCTGGATTCATCTAATAGTACCATTGCCATTTT; ACAAACTGAGAAAGACAAACTAGAGTTGGAAATTACAGATTCTAAAAAAGAACAAGATGATCTCTTGGTG; CTCTTGGCCGATCAAGATCAGAAAATACTGTCATTGAAGAATAAACTCAAGGATCTTGGTCATCCAGTTG; AAGAAGAGGATGAACTTGAATCTGGAGACCAAGAGGATGAGGATGATGAAAGTGAAGATCCTGGCAAGGA; TCTAGATCATATC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207614 representing NM_003715; Red=Cloning site Green=Tags(s); ; MNFLRGVMGGQSAGPQHTEAETIQKLCDRVASSTLLDDRRNAVRALKSLSKKYRLEVGIQAMEHLIHVLQ; TDRSDSEIIGYALDTLYNIISNEEEEVEENSTRQSEDLGSQFTEIFIKQQENVTLLLSLLEEFDFHVRWP; GVKLLTSLLKQLGPQVQQIILVSPMGVSRLMDLLADSREVIRNDGVLLLQALTRSNGAIQKIVAFENAFE; RLLDIISEEGNSDGGIVVEDCLILLQNLLKNNNSNQNFFKEGSYIQRMKPWFEVGDENSGWSAQKVTNLH; LMLQLVRVLVSPTNPPGATSSCQKAMFQCGLLQQLCTILMATGVPADILTETINTVSEVIRGCQVNQDYF; ASVNAPSNPPRPAIVVLLMSMVNERQPFVLRCAVLYCFQCFLYKNQKGQGEIVSTLLPSTIDATGNSVSA; GQLLCGGLFSTDSLSNWCAAVALAHALQENATQKEQLLRVQLATSIGNPPVSLLQQCTNILSQGSKIQTR; VGLLMLLCTWLSNCPIAVTHFLHNSANVPFLTGQIAENLGEEEQLVQGLCALLLGISIYFNDNSLESYMK; EKLKQLIEKRIGKENFIEKLGFISKHELYSRASQKPQPNFPSPEYMIFDHEFTKLVKELEGVITKAIYKS; SEEDKKEEEVKKTLEQHDNIVTHYKNMIREQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQY; NLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGT; NEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIAT; KTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLV; LLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003715
• ORF Size: 2883 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003715.2, NP_003706.1
• RefSeq Size: 3998 bp
• RefSeq ORF: 2889 bp
• Locus ID: 8615
• UniProt ID: O60763
• Cytogenetics: 4q21.1
• Domains: Uso1_p115_C, Uso1_p115_head
• Summary: The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]