SP2 transcription factor (SP2) (NM_003110) Human Tagged ORF Clone

SKU RG207602

SP2 (tGFP-tagged) - Human Sp2 transcription factor (SP2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SP2 transcription factor
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207602 representing NM_003110; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTGCCACTGCTGCTGTGAGTCCCAGTGACTACCTGCAGCCTGCCGCCTCCACCACCCAGGACTCCC; AGCCATCTCCCTTAGCCCTGCTTGCTGCAACATGTAGCAAAATTGGCCCTCCAGCAGTTGAAGCTGCTGT; GACACCTCCTGCTCCCCCACAGCCCACACCGCGGAAACTTGTCCCTATCAAACCTGCCCCTCTCCCTCTC; AGCCCCGGCAAGAATAGCTTTGGAATCTTGTCCTCCAAAGGAAATATACTTCAGATTCAGGGGTCACAAC; TGAGCGCCTCCTATCCTGGAGGGCAGCTGGTGTTCGCTATCCAGAATCCCACCATGATCAACAAAGGGAC; CCGATCAAATGCCAATATCCAGTACCAGGCGGTCCCTCAGATTCAGGCAAGCAATTCCCAAACCATCCAA; GTACAGCCCAATCTCACCAACCAGATCCAGATCATCCCTGGCACCAACCAAGCCATCATCACCCCCTCAC; CGTCCAGTCACAAGCCTGTCCCCATCAAGCCAGCCCCCATCCAGAAGTCGAGTACGACCACCACCCCCGT; GCAGAGCGGGGCCAATGTGGTGAAGTTGACAGGTGGGGGCGGCAATGTGACGCTCACTCTGCCCGTCAAC; AACCTCGTGAACGCCAGTGACACCGGGGCCCCTACTCAGCTCCTCACTGAAAGCCCCCCAACCCCACTGT; CTAAGACTAACAAGAAAGCAAGGAAGAAGAGCCTTCCTGCCTCCCAGCCCCCTGTGGCTGTGGCTGAGCA; GGTGGAGACGGTGCTGATCGAGACCACCGCGGACAACATCATCCAGGCAGGAAATAACCTGCTCATTGTT; CAGAGCCCTGGTGGGGGCCAGCCAGCTGTGGTCCAGCAGGTCCAGGTGGTGCCCCCCAAGGCCGAGCAGC; AGCAGGTGGTACAGATCCCCCAGCAGGCTCTGCGGGTGGTGCAGGCGGCATCTGCCACCCTCCCCACTGT; ACCCCAGAAGCCCTCCCAGAACTTTCAGATCCAGGCAGCTGAGCCGACACCTACTCAGGTCTACATCCGC; ACGCCTTCCGGTGAGGTGCAGACAGTCCTTGTCCAGGACAGCCCCCCAGCAACAGCTGCAGCCACCTCTA; ACACCACCTGTAGCAGCCCTGCATCCCGTGCTCCCCATCTGAGTGGGACCAGCAAAAAGCACTCAGCTGC; AATTCTCCGAAAAGAGCGTCCCCTGCCAAAGATTGCCCCAGCCGGGAGCATCATCAGCCTGAATGCAGCC; CAGTTGGCGGCAGCTGCCCAGGCAATGCAGACCATCAACATCAATGGTGTCCAGGTCCAGGGCGTGCCTG; TCACCATCACCAACACAGGCGGGCAGCAGCAGCTGACAGTGCAGAATGTTTCTGGGAACAACCTGACCAT; CAGTGGGCTGAGCCCCACCCAGATCCAGCTGCAAATGGAACAAGCCCTGGCCGGAGAGACCCAGCCCGGG; GAGAAGCGGCGCCGCATGGCCTGCACGTGTCCCAACTGCAAGGATGGGGAGAAGAGGTCTGGAGAGCAGG; GCAAGAAGAAGCACGTTTGCCACATCCCCGACTGTGGCAAGACGTTCCGTAAGACGTCCTTGCTGCGTGC; CCATGTGCGCCTGCACACTGGCGAGCGGCCCTTTGTCTGCAACTGGTTCTTCTGTGGGAAGAGGTTCACA; CGGAGTGACGAGCTCCAACGGCATGCTCGCACCCACACAGGGGACAAACGCTTCGAGTGCGCCCAGTGTC; AGAAGCGCTTCATGAGGAGTGACCACCTCACCAAGCATTACAAGACCCACCTGGTCACGAAGAACTTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207602 representing NM_003110; Red=Cloning site Green=Tags(s); ; MAATAAVSPSDYLQPAASTTQDSQPSPLALLAATCSKIGPPAVEAAVTPPAPPQPTPRKLVPIKPAPLPL; SPGKNSFGILSSKGNILQIQGSQLSASYPGGQLVFAIQNPTMINKGTRSNANIQYQAVPQIQASNSQTIQ; VQPNLTNQIQIIPGTNQAIITPSPSSHKPVPIKPAPIQKSSTTTTPVQSGANVVKLTGGGGNVTLTLPVN; NLVNASDTGAPTQLLTESPPTPLSKTNKKARKKSLPASQPPVAVAEQVETVLIETTADNIIQAGNNLLIV; QSPGGGQPAVVQQVQVVPPKAEQQQVVQIPQQALRVVQAASATLPTVPQKPSQNFQIQAAEPTPTQVYIR; TPSGEVQTVLVQDSPPATAAATSNTTCSSPASRAPHLSGTSKKHSAAILRKERPLPKIAPAGSIISLNAA; QLAAAAQAMQTININGVQVQGVPVTITNTGGQQQLTVQNVSGNNLTISGLSPTQIQLQMEQALAGETQPG; EKRRRMACTCPNCKDGEKRSGEQGKKKHVCHIPDCGKTFRKTSLLRAHVRLHTGERPFVCNWFFCGKRFT; RSDELQRHARTHTGDKRFECAQCQKRFMRSDHLTKHYKTHLVTKNL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003110
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003110.4, NP_003101.2
• RefSeq Size: 3132 bp
• RefSeq ORF: 1842 bp
• Locus ID: 6668
• UniProt ID: Q02086
• Cytogenetics: 17q21.32
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Summary: This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein contains the least conserved DNA-binding domain within the Sp subfamily of proteins, and its DNA sequence specificity differs from the other Sp proteins. It localizes primarily within subnuclear foci associated with the nuclear matrix, and can activate or in some cases repress expression from different promoters. [provided by RefSeq, Jul 2008]