DIP13B (APPL2) (NM_018171) Human Tagged ORF Clone

SKU RG207506

APPL2 (tGFP-tagged) - Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: DIP13B
• Synonyms: DIP13B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG207506 representing NM_018171; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCCGCCGTGGACAAGCTCCTGCTAGAGGAGGCGTTGCAGGACAGCCCCCAGACTCGCTCTTTACTGA; GCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATACCAACCAGCTGCTCCAGGCAATGCAGCGCGT; CTATGGAGCCCAGAATGAGATGTGCCTGGCCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAA; CAGAACTTTGCTCTTGGCAAAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGG; ATGAGCTTAATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATACA; ATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTAGCAATGAGCAT; GACCTCTCAATGGCAAAATACAGCAGGCTGCCTAAGAAAAAGGAGAATGAGAAGGTGAAGACCGAAGTCG; GAAAAGAGGTGGCCGCGGCCCGGCGGAAGCAGCATCTCTCCTCCCTTCAGTACTACTGTGCCCTCAACGC; GCTGCAGTACAGAAAGCAAATGGCCATGATGGAGCCCATGATAGGCTTTGCCCATGGACAGATTAACTTT; TTTAAGAAGGGAGCAGAGATGTTTTCCAAACGTATGGACAGCTTTTTATCCTCCGTTGCAGACATGGTTC; AAAGCATTCAGGTAGAACTGGAAGCCGAGGCGGAAAAGATGCGGGTGTCCCAGCAAGAATTACTTTCTGT; TGATGAATCTGTTTACACTCCAGACTCTGATGTGGCCGCACCACAGATCAACAGGAACCTCATCCAGAAG; GCTGGTTACCTTAATCTTAGAAACAAAACAGGGCTGGTCACCACCACCTGGGAGAGGCTTTATTTCTTCA; CCCAAGGCGGGAATCTCATGTGTCAGCCCAGGGGAGCCGTGGCTGGAGGTTTGATCCAGGACCTGGACAA; CTGCTCAGTGATGGCCGTGGATTGCGAAGACCGGCGCTACTGCTTCCAGATCACCACGCCCAATGGAAAA; TCGGGAATAATCCTCCAGGCTGAGAGCAGAAAGGAAAATGAAGAGTGGATATGTGCAATAAACAACATCT; CCAGACAGATCTACCTGACCGACAACCCTGAGGCAGTCGCGATCAAGTTGAATCAGACCGCTCTGCAAGC; AGTGACTCCCATTACAAGTTTTGGAAAAAAACAAGAAAGCTCATGCCCCAGCCAGAACCTGAAAAATTCA; GAGATGGAAAATGAAAATGACAAGATTGTTCCCAAAGTAACAGCCAGTCTACCTGAAGCAGAGGAGCTGA; TCGCGCCTGGAACGCCGATTCAATTCGATATTGTGCTTCCTGCTACAGAATTCCTTGATCAGAACAGAGG; GAGCAGGCGTACCAACCCTTTTGGTGAAACTGAGGATGAATCATTTCCAGAAGCAGAAGATTCTCTTTTG; CAGCAGATGTTTATAGTTCGGTTTTTGGGATCAATGGCAGTTAAAACAGACAGCACTACTGAAGTGATTT; ATGAAGCGATGAGACAAGTATTGGCTGCTCGGGCTATTCATAACATCTTCCGCATGACAGAATCCCATCT; GATGGTCACCAGTCAATCTTTGAGGTTGATAGATCCACAGACTCAAGTATCAAGGGCCAATTTTGAACTT; ACCAGTGTCACACAATTTGCTGCTCATCAAGAAAACAAGAGACTGGTTGGTTTTGTCATCCGTGTTCCTG; AATCCACTGGAGAAGAATCTCTGAGTACATACATTTTTGAAAGCAACTCAGAAGGCGAAAAGATATGTTA; TGCTATTAATTTGGGAAAAGAAATTATTGAGGTTCAGAAGGATCCAGAAGCACTGGCTCAATTAATGCTG; TCCATACCACTAACCAATGATGGAAAATATGTACTGTTAAACGATCAACCAGATGACGATGATGGAAATC; CAAATGAACATAGAGGCGCAGAATCCGAAGCA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG207506 representing NM_018171; Red=Cloning site Green=Tags(s); ; MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEK; QNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEH; DLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINF; FKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQK; AGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGK; SGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNS; EMENENDKIVPKVTASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLL; QQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFEL; TSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLML; SIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018171
• ORF Size: 1992 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018171.3, NP_060641.2
• RefSeq Size: 3289 bp
• RefSeq ORF: 1995 bp
• Locus ID: 55198
• UniProt ID: Q8NEU8
• Cytogenetics: 12q23.3
• Domains: PH, PID
• Summary: The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011]