MSL3L1 (MSL3) (NM_078629) Human Tagged ORF Clone

SKU RG206888

MSL3 (tGFP-tagged) - Human male-specific lethal 3 homolog (Drosophila) (MSL3), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MSL3L1
• Synonyms: MRSXBA; MRXS36; MRXSBA; MSL3L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206888 representing NM_078629; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCGCGAGCGAGGGCATGAAATTTAAATTCCACTCAGGGGAGAAAGTGCTGTGCTTCGAGCCTGACC; CCACCAAGGCGCGAGTGCTGTACGATGCCAAGATTGTTGATGTTATTGTTGGGAAAGACGAAAAAGGCAG; AAAGATCCCAGAATATCTGATCCATTTTAATGGTTGGAACAGAAGCTGGGATAGATGGGCAGCAGAAGAT; CATGTGCTTCGTGATACCGATGAAAATCGTAGATTACAGCGTAAATTGGCAAGAAAAGCTGTAGCTCGCC; TGAGGAGCACAGGAAGAAAGAAGAAGCGCTGCAGGTTGCCTGGTGTGGACTCTGTCTTAAAAGGCCTCCC; CACTGAAGAAAAAGATGAAAATGATGAAAACTCATTAAGCAGTTCCTCTGACTGTAGTGAAAACAAGGAT; GAAGAAATAAGTGAAGAAAGTGATATTGAAGAAAAGACTGAAGTGAAAGAAGAACCAGAGCTTCAAACAA; GAAGGGAAATGGAAGAAAGAACAATAACTATAGAAATCCCTGAAGTTCTGAAGAAGCAGCTGGAGGATGA; TTGTTACTACATTAACAGGAGGAAACGGTTAGTGAAACTTCCATGCCAGACCAACATCATAACGATTTTG; GAATCCTATGTGAAGCATTTTGCTATCAATGCAGCCTTTTCAGCCAATGAGAGGCCTCGTCACCATCACG; TTATGCCACATGCCAACATGAACGTGCATTATATCCCAGCAGAAAAGAATGTTGACCTTTGTAAGGAGAT; GGTGGATGGATTAAGAATAACCTTTGATTACACTCTCCCGTTGGTTTTACTCTATCCATATGAACAAGCT; CAGTATAAAAAGGTGACTTCGTCTAAATTTTTTCTTCCAATTAAGGAAAGTGCCACAAGCACTAACAGGA; GCCAGGAGGAACTCTCTCCCAGTCCGCCTTTGTTGAATCCATCCACGCCACAGTCCACAGAGAGTCAGCC; GACCACCGGTGAACCAGCCACCCCCAAAAGGCGCAAAGCTGAGCCAGAAGCATTGCAGTCTCTGAGGCGG; TCCACGCGCCACAGTGCCAACTGTGACAGGCTTTCTGAGAGCAGCGCTTCACCTCAGCCCAAGCGCCGGC; AGCAGGACACATCCGCCAGCATGCCCAAGCTCTTCCTGCACCTGGAAAAGAAGACACCTGTGCATAGCAG; ATCATCTTCACCTATTCCTCTGACTCCTAGCAAGGAAGGGAGTGCTGTGTTTGCTGGCTTTGAAGGGAGA; AGAACTAATGAAATAAACGAGGTCCTCTCCTGGAAGCTTGTGCCTGACAATTACCCCCCAGGTGACCAGC; CGCCTCCACCCTCTTACATTTATGGGGCACAACATTTGCTGCGATTGTTTGTGAAACTTCCAGAAATCCT; TGGAAAGATGTCCTTTTCTGAGAAGAATCTGAAGGCTTTATTGAAGCACTTTGATCTCTTTTTGAGGTTT; TTAGCAGAATACCACGATGACTTCTTCCCAGAGTCGGCTTATGTCGCTGCCTGTGAGGCACATTACAGCA; CCAAGAACCCCCGGGCAATTTAT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206888 representing NM_078629; Red=Cloning site Green=Tags(s); ; MSASEGMKFKFHSGEKVLCFEPDPTKARVLYDAKIVDVIVGKDEKGRKIPEYLIHFNGWNRSWDRWAAED; HVLRDTDENRRLQRKLARKAVARLRSTGRKKKRCRLPGVDSVLKGLPTEEKDENDENSLSSSSDCSENKD; EEISEESDIEEKTEVKEEPELQTRREMEERTITIEIPEVLKKQLEDDCYYINRRKRLVKLPCQTNIITIL; ESYVKHFAINAAFSANERPRHHHVMPHANMNVHYIPAEKNVDLCKEMVDGLRITFDYTLPLVLLYPYEQA; QYKKVTSSKFFLPIKESATSTNRSQEELSPSPPLLNPSTPQSTESQPTTGEPATPKRRKAEPEALQSLRR; STRHSANCDRLSESSASPQPKRRQQDTSASMPKLFLHLEKKTPVHSRSSSPIPLTPSKEGSAVFAGFEGR; RTNEINEVLSWKLVPDNYPPGDQPPPPSYIYGAQHLLRLFVKLPEILGKMSFSEKNLKALLKHFDLFLRF; LAEYHDDFFPESAYVAACEAHYSTKNPRAIY; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_078629
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_078629.4
• RefSeq Size: 2343 bp
• RefSeq ORF: 1566 bp
• Locus ID: 10943
• UniProt ID: Q8N5Y2
• Cytogenetics: Xp22.2
• Domains: CHROMO
• Protein Families: Transcription Factors
• Summary: This gene encodes a nuclear protein that is similar to the product of the Drosophila male-specific lethal-3 gene. The Drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus, the human protein is thought to play a similar function in chromatin remodeling and transcriptional regulation, and it has been found as part of a complex that is responsible for histone H4 lysine-16 acetylation. This gene can undergo X inactivation. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 7 and 8. [provided by RefSeq, Jul 2010]