SUNC1 (SUN3) (NM_152782) Human Tagged ORF Clone

SKU
RG206129
SUN3 (tGFP-tagged) - Human Sad1 and UNC84 domain containing 3 (SUN3), transcript variant 2
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $657.00 MSRP $657.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SUNC1
Synonyms SUNC1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG206129 representing NM_152782
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGAAAAACAAAGGCAAGAAGGGCTGCCATGTTTTTTAGACGTTGCTCTGAAGACGCCAGCGGTA
GCGCCAGTGGCAATGCTTTGTTATCAGAGGACGAAAATCCTGATGCGAATGGGGTAACTCGATCATGGAA
GATTATTCTAAGTACAATGCTTACACTGACTTTTCTTCTTGTAGGACTCCTAAATCATCAGTGGCTTAAA
GAAACAGATGTTCCTCAGAAATCCAGACAATTATATGCCATAATTGCAGAATATGGTTCAAGGCTTTATA
AATATCAGGCCAGACTTCGTATGCCTAAAGAGCAACTGGAACTTTTAAAGAAGGAAAGCCAGAATCTGGA
AAACAATTTTCGTCAAATTCTATTTTTGGTCGAACAAATAGATGTCCTGAAGGCATTGCTAAGAGATATG
AAGGATGGTATGGACAATAATCACAACTGGAACACCCATGGAGACCCTGTGGAGGACCCGGACCACACAG
AGGAAGTGTCAAACTTGGTCAATTATGTACTTAAAAAGTTGAGAGAAGACCAAGTCGAGATGGCTGATTA
TGCCCTGAAGTCGGCCGGAGCCTCCATCATTGAAGCTGGGACCTCAGAAAGTTATAAAAATAATAAAGCA
AAATTGTACTGGCATGGGATAGGTTTCCTAAATCATGAAATGCCTCCAGATATTATTCTTCAGCCGGATG
TCTACCCTGGAAAGTGCTGGGCTTTTCCAGGTTCCCAGGGTCATACCCTAATCAAGCTTGCTACAAAGAT
CATACCAACTGCTGTTACCATGGAGCACATCTCAGAGAAGGTGTCTCCGTCAGGAAACATCTCCAGTGCA
CCCAAGGAATTTTCTGTCTATGGCATCACAAAAAAATGTGAAGGAGAAGAAATTTTCCTAGGTCAGTTTA
TATATAACAAAACAGGAACCACCGTTCAAACATTTGAACTCCAGCATGCAGTTTCTGAATATTTATTATG
TGTGAAACTTAATATCTTTAGCAACTGGGGACACCCGAAGTATACTTGTTTATATCGATTCAGGGTCCAT
GGCACACCAGGCAAGCACATC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG206129 representing NM_152782
Red=Cloning site Green=Tags(s)

MSGKTKARRAAMFFRRCSEDASGSASGNALLSEDENPDANGVTRSWKIILSTMLTLTFLLVGLLNHQWLK
ETDVPQKSRQLYAIIAEYGSRLYKYQARLRMPKEQLELLKKESQNLENNFRQILFLVEQIDVLKALLRDM
KDGMDNNHNWNTHGDPVEDPDHTEEVSNLVNYVLKKLREDQVEMADYALKSAGASIIEAGTSESYKNNKA
KLYWHGIGFLNHEMPPDIILQPDVYPGKCWAFPGSQGHTLIKLATKIIPTAVTMEHISEKVSPSGNISSA
PKEFSVYGITKKCEGEEIFLGQFIYNKTGTTVQTFELQHAVSEYLLCVKLNIFSNWGHPKYTCLYRFRVH
GTPGKHI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_152782
ORF Size 1071 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152782.3, NP_689995.3
RefSeq Size 1368 bp
RefSeq ORF 1074 bp
Locus ID 256979
UniProt ID Q8TAQ9
Cytogenetics 7p12.3
Summary As a probable component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nuclear remodeling during sperm head formation in spermatogenenis. A probable SUN3:SYNE1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.[UniProtKB/Swiss-Prot Function]
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Citations

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