DAZL (NM_001351) Human Tagged ORF Clone

SKU
RG205328
DAZL (tGFP-tagged) - Human deleted in azoospermia-like (DAZL), transcript variant 2
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

    Click here to learn more.

$489.00 MSRP $500.00 MSRP $500.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DAZL
Synonyms DAZH; DAZL1; DAZLA; SPGYLA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG205328 representing NM_001351
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTACTGCAAATCCTGAAACTCCAAACTCAACCATCTCCAGAGAGGCCAGCACCCAGTCCTCATCAG
CTGCAACCAGCCAAGGCTATATTTTACCAGAAGGCAAAATCATGCCAAACACTGTTTTTGTTGGAGGAAT
TGATGTTAGGATGGATGAAACTGAGATTAGAAGCTTCTTTGCTAGATATGGTTCAGTGAAAGAAGTGAAG
ATAATCACTGATCGAACTGGTGTGTCCAAAGGCTATGGATTTGTTTCATTTTTTAATGACGTGGATGTGC
AGAAGATAGTAGAATCACAGATAAATTTCCATGGTAAAAAGCTGAAGCTGGGCCCTGCAATCAGGAAACA
AAATTTATGTGCTTATCATGTGCAGCCACGTCCTTTGGTTTTTAATCATCCTCCTCCACCACAGTTTCAG
AATGTCTGGACTAATCCAAACACTGAAACTTATATGCAGCCCACAACCACGATGAATCCTATAACTCAGT
ATGTTCAGGCATATCCTACTTACCCAAATTCACCAGTTCAGGTCATCACTGGATATCAGTTGCCTGTATA
TAATTATCAGATGCCACCACAGTGGCCTGTTGGGGAGCAAAGGAGCTATGTTGTACCTCCGGCTTATTCA
GCTGTTAACTACCACTGTAATGAAGTTGATCCAGGAGCTGAAGTTGTGCCAAATGAATGTTCAGTTCATG
AAGCTACTCCACCCTCTGGAAATGGCCCACAAAAGAAATCTGTGGACCGAAGCATACAAACGGTGGTATC
TTGTCTGTTTAATCCAGAGAACAGACTGAGAAACTCTGTTGTTACTCAAGATGACTACTTCAAGGATAAA
AGAGTGCATCACTTTAGAAGAAGTCGGGCAATGCTTAAATCTGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG205328 representing NM_001351
Red=Cloning site Green=Tags(s)

MSTANPETPNSTISREASTQSSSAATSQGYILPEGKIMPNTVFVGGIDVRMDETEIRSFFARYGSVKEVK
IITDRTGVSKGYGFVSFFNDVDVQKIVESQINFHGKKLKLGPAIRKQNLCAYHVQPRPLVFNHPPPPQFQ
NVWTNPNTETYMQPTTTMNPITQYVQAYPTYPNSPVQVITGYQLPVYNYQMPPQWPVGEQRSYVVPPAYS
AVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDRSIQTVVSCLFNPENRLRNSVVTQDDYFKDK
RVHHFRRSRAMLKSV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001351
ORF Size 885 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001351.4
RefSeq Size 3056 bp
RefSeq ORF 888 bp
Locus ID 1618
UniProt ID Q92904
Cytogenetics 3p24.3
Summary The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Write Your Own Review
You're reviewing:DAZL (NM_001351) Human Tagged ORF Clone
Your Rating
SKU Description Size Price
RC205328 DAZL (Myc-DDK-tagged)-Human deleted in azoospermia-like (DAZL), transcript variant 2 10 ug
$300.00
RC205328L1 Lenti ORF clone of Human deleted in azoospermia-like (DAZL), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC205328L2 Lenti ORF clone of Human deleted in azoospermia-like (DAZL), transcript variant 2, mGFP tagged 10 ug
$600.00
RC205328L3 Lenti ORF clone of Human deleted in azoospermia-like (DAZL), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC205328L4 Lenti ORF clone of Human deleted in azoospermia-like (DAZL), transcript variant 2, mGFP tagged 10 ug
$600.00
SC303003 DAZL (untagged)-Human deleted in azoospermia-like (DAZL), transcript variant 2 10 ug
$330.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.