TTC35 (EMC2) (NM_014673) Human Tagged ORF Clone

SKU RG204770

EMC2 (tGFP-tagged) - Human tetratricopeptide repeat domain 35 (TTC35)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TTC35
• Synonyms: KIAA0103; TTC35
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204770 representing NM_014673; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGAAGGTCTCAGAGCTTTACGATGTCACTTGGGAAGAAATGAGAGATAAAATGAGAAAATGGAGAG; AAGAAAACTCAAGAAATAGTGAGCAAATTGTGGAAGTTGGAGAAGAATTAATTAATGAATATGCTTCTAA; GCTGGGAGATGATATTTGGATCATATATGAACAGGTGATGATTGCAGCACTAGACTATGGTCGGGATGAC; TTGGCATTGTTTTGTCTTCAAGAGCTGAGAAGACAGTTCCCTGGCAGTCACAGAGTCAAGCGATTAACAG; GCATGAGATTTGAAGCCATGGAAAGATATGATGATGCTATACAGCTATATGATAGGATTTTACAAGAAGA; TCCAACTAACACTGCTGCAAGAAAGCGTAAGATTGCCATTCGAAAAGCCCAGGGGAAAAATGTGGAGGCC; ATTCGGGAGCTGAATGAGTATCTGGAACAATTTGTTGGAGACCAAGAAGCCTGGCATGAACTTGCAGAAC; TTTACATCAATGAACATGACTATGCAAAAGCAGCCTTTTGTTTAGAGGAACTAATGATGACTAATCCACA; CAACCACTTATACTGTCAGCAGTATGCTGAAGTTAAGTATACCCAAGGTGGACTTGAAAACCTCGAACTT; TCAAGAAAGTATTTTGCACAGGCATTGAAACTGAACAACAGAAATATGAGAGCTTTGTTTGGACTTTATA; TGTCGGCAAGTCATATTGCTTCTAATCCAAAAGCAAGTGCAAAAACGAAAAAGGACAACATGAAATATGC; TAGTTGGGCAGCTAGTCAAATAAACAGAGCTTATCAGTTTGCAGGTCGAAGTAAGAAGGAAACCAAATAT; TCTCTTAAGGCTGTCGAAGACATGTTGGAAACATTGCAGATCACCCAGTCT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204770 representing NM_014673; Red=Cloning site Green=Tags(s); ; MAKVSELYDVTWEEMRDKMRKWREENSRNSEQIVEVGEELINEYASKLGDDIWIIYEQVMIAALDYGRDD; LALFCLQELRRQFPGSHRVKRLTGMRFEAMERYDDAIQLYDRILQEDPTNTAARKRKIAIRKAQGKNVEA; IRELNEYLEQFVGDQEAWHELAELYINEHDYAKAAFCLEELMMTNPHNHLYCQQYAEVKYTQGGLENLEL; SRKYFAQALKLNNRNMRALFGLYMSASHIASNPKASAKTKKDNMKYASWAASQINRAYQFAGRSKKETKY; SLKAVEDMLETLQITQS; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014673
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014673.5
• RefSeq Size: 1273 bp
• RefSeq ORF: 894 bp
• Locus ID: 9694
• UniProt ID: Q15006
• Cytogenetics: 8q23.1
• Domains: TPR
• Protein Families: Protease
• Summary: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656). Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231). Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151). It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231). By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835). By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).[UniProtKB/Swiss-Prot Function]