MTBP (NM_022045) Human Tagged ORF Clone

SKU
RG204388
MTBP (tGFP-tagged) - Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP)
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    Expression-ready ORF plasmid with C-terminal tag(s)

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$1,028.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MTBP
Synonyms MDM2BP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG204388 representing NM_022045
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCGGTACCTGCTGCTGGTGATCTGGGGGGAAGGAAAATTCCCGTCGGCGGCCAGTAGGGAGGCAG
AACATGGGCCAGAGGTGTCGTCGGGTGAGGGTACTGAGAATCAGCCGGACTTCACAGCAGCAAATGTTTA
TCACCTCTTGAAAAGAAGCATTAGTGCTTCAATTAATCCAGAAGATAGTACTTTCCCTGCCTGTTCAGTG
GGAGGTATACCTGGTTCCAAGAAGTGGTTCTTTGCAGTGCAGGCAATATATGGATTTTATCAGTTTTGTA
GTTCTGATTGGCAAGAGATACATTTTGATACAGAAAAAGATAAAATTGAAGATGTTCTTCAAACGAATAT
CGAAGAATGTTTGGGTGCTGTTGAGTGTTTTGAAGAAGAAGACAGTAATAGCAGGGAATCATTATCCTTG
GCTGATCTCTATGAAGAAGCTGCAGAAAATTTGCATCAGCTGTCAGACAAGCTTCCTGCTCCTGGTAGAG
CAATGGTAGATATAATACTGTTGCTTTCTGACAAAGATCCTCCTAAATTGAAAGACTATTTACCTACTGT
AGGAGCATTAAAACATTTGAGAGAATGGTATTCAGCAAAGATCACTATAGCAGGAAATCATTGTGAAATA
AACTGTCAGAAAATTGCAGAATACCTTTCTGCTAATGTTGTATCTTTAGAAGATCTCAGAAATGTTATTG
ACTCAAAGGAATTATGGAGGGGGAAAATACAGATATGGGAAAGAAAGTTTGGATTTGAAATTAGTTTTCC
TGAATTTTGTTTAAAGGGAGTCACACTTAAGAATTTTAGTACTTCTAATTTAAATACTGACTTCCTTGCC
AAAAAGATCATACCATCAAAGGATAAGAATATTTTGCCAAAGGTTTTCCATTATTATGGCCCTGCTTTAG
AATTTGTGCAGATGATAAAATTATCAGATCTACCCTCCTGCTATATGTCGGATATTGAATTTGAGTTAGG
ATTGACAAACAGTACCAAACAGAATTCTGTGTTGCTGTTGGAGCAGATTTCTTCTCTGTGTAGCAAGGTT
GGTGCTCTTTTTGTATTGCCATGTACCATTAGTAACATACTGATTCCACCTCCCAACCAACTCAGTTCAA
GAAAATGGAAGGAATATATAGCTAAAAAGCCTAAAACAATCAGTGTTCCAGATGTTGAAGTGAAAGGAGA
GTGTTCTAGCTATTATCTCTTGTTACAAGGTAATGGCAATAGAAGATGTAAAGCCACATTGATTCACTCA
GCCAACCAGATCAATGGCTCATTTGCACTCAATTTAATTCATGGAAAGATGAAAACAAAGACAGAAGAAG
CCAAATTGAGTTTTCCTTTTGACTTATTATCACTTCCACATTTTTCTGGGGAGCAGATTGTACAGAGAGA
GAAACAGTTAGCTAATGTTCAAGTTTTAGCTTTGGAAGAATGCCTAAAAAGACGAAAGTTGGCAAAGCAG
CCTGAAACAGTTTCTGTTGCTGAACTCAAAAGTCTGTTAGTACTCACAAGGAAACACTTTTTAGATTATT
TTGATGCTGTGATTCCTAAAATGATTCTAAGAAAGATGGACAAAATTAAAACCTTCAATATATTAAATGA
TTTTAGTCCAGTGGAACCTAATTCCTCAAGTCTAATGGAAACCAATCCTCTGGAATGGCCAGAAAGGCAT
GTTCTTCAAAATTTGGAAACTTTTGAAAAAACTAAACAAAAAATGAGAACTGGTTCATTACCTCATTCAT
CTGAACAGTTGCTGGGCCACAAAGAGGGTCCTCGGGACTCAATCACATTGTTGGATGCTAAAGAATTGCT
GAAGTACTTTACCTCAGATGGATTACCCATTGGAGATCTTCAACCTTTACCGATTCAAAAGGGGGAAAAG
ACTTTTGTTTTGACACCAGAACTTAGTCCTGGGAAACTTCAGGTCTTACCTTTTGAGAAAGCCTCAGTAT
GTCATTATCATGGAATTGAATATTGCTTGGATGACCGAAAAGCTTTGGAAAGAGATGGAGGATTTTCTGA
ACTTCAGTCTCGTCTTATTCGTTATGAAACTCAAACTACCTGCACCAGAGAAAGTTTTCCAGTACCTACT
GTGTTGAGCCCTCTTCCATCTCCTGTAGTTTCGTCAGATCCTGGAAGTGTCCCTGACGGAGAAGTTTTAC
AAAATGAACTTCGAACTGAAGTATCCCGATTGAAACGGAGATCTAAAGATCTGAATTGCCTTTATCCCAG
AAAAAGACTTGTGAAATCTGAAAGTTCAGAGTCTCTTCTTTCTCAGACAACTGGTAATAGTAATCACTAT
CATCATCATGTGACATCCAGAAAGCCACAAACAGAACGGTCCTTACCAGTGACTTGTCCATTGGTTCCAA
TTCCTAGCTGTGAAACTCCAAAACTTGCTACAAAGACCAGTTCAGGTCAAAAAAGTATGCATGAATCAAA
AACATCAAGGCAAATTAAGGAATCAAGATCACAGAAACACACACGGATACTGAAAGAAGTAGTTACTGAA
ACCCTGAAGAAACACAGTATTACCGAGACTCATGAATGTTTCACTGCATGCAGCCAGCGTCTCTTTGAAA
TCTCTAAGTTCTATCTAAAGGATCTTAAAACTTCAAGGGGTCTATTTGAAGAAATGAAGAAAACAGCAAA
CAACAATGCTGTACAGGTGATTGACTGGGTATTAGAAAAGACAAGCAAGAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG204388 representing NM_022045
Red=Cloning site Green=Tags(s)

MDRYLLLVIWGEGKFPSAASREAEHGPEVSSGEGTENQPDFTAANVYHLLKRSISASINPEDSTFPACSV
GGIPGSKKWFFAVQAIYGFYQFCSSDWQEIHFDTEKDKIEDVLQTNIEECLGAVECFEEEDSNSRESLSL
ADLYEEAAENLHQLSDKLPAPGRAMVDIILLLSDKDPPKLKDYLPTVGALKHLREWYSAKITIAGNHCEI
NCQKIAEYLSANVVSLEDLRNVIDSKELWRGKIQIWERKFGFEISFPEFCLKGVTLKNFSTSNLNTDFLA
KKIIPSKDKNILPKVFHYYGPALEFVQMIKLSDLPSCYMSDIEFELGLTNSTKQNSVLLLEQISSLCSKV
GALFVLPCTISNILIPPPNQLSSRKWKEYIAKKPKTISVPDVEVKGECSSYYLLLQGNGNRRCKATLIHS
ANQINGSFALNLIHGKMKTKTEEAKLSFPFDLLSLPHFSGEQIVQREKQLANVQVLALEECLKRRKLAKQ
PETVSVAELKSLLVLTRKHFLDYFDAVIPKMILRKMDKIKTFNILNDFSPVEPNSSSLMETNPLEWPERH
VLQNLETFEKTKQKMRTGSLPHSSEQLLGHKEGPRDSITLLDAKELLKYFTSDGLPIGDLQPLPIQKGEK
TFVLTPELSPGKLQVLPFEKASVCHYHGIEYCLDDRKALERDGGFSELQSRLIRYETQTTCTRESFPVPT
VLSPLPSPVVSSDPGSVPDGEVLQNELRTEVSRLKRRSKDLNCLYPRKRLVKSESSESLLSQTTGNSNHY
HHHVTSRKPQTERSLPVTCPLVPIPSCETPKLATKTSSGQKSMHESKTSRQIKESRSQKHTRILKEVVTE
TLKKHSITETHECFTACSQRLFEISKFYLKDLKTSRGLFEEMKKTANNNAVQVIDWVLEKTSKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_022045
ORF Size 2712 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022045.5
RefSeq Size 3081 bp
RefSeq ORF 2715 bp
Locus ID 27085
UniProt ID Q96DY7
Cytogenetics 8q24.12
Summary This gene encodes a protein that interacts with the oncoprotein mouse double minute 2. The encoded protein regulates progression through the cell cycle and may be involved in tumor formation. [provided by RefSeq, Aug 2012]
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SKU Description Size Price
RC204388 MTBP (Myc-DDK-tagged)-Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP) 10 ug
$828.00
RC204388L1 Lenti ORF clone of Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP), Myc-DDK-tagged 10 ug
$1,128.00
RC204388L2 Lenti ORF clone of Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP), mGFP tagged 10 ug
$1,128.00
RC204388L3 Lenti ORF clone of Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP), Myc-DDK-tagged 10 ug
$1,128.00
RC204388L4 Lenti ORF clone of Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP), mGFP tagged 10 ug
$1,128.00
SC112852 MTBP (untagged)-Human Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa (MTBP) 10 ug
$829.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.