GLUT8 (SLC2A8) (NM_014580) Human Tagged ORF Clone

SKU RG204317

SLC2A8 (tGFP-tagged) - Human solute carrier family 2 (facilitated glucose transporter), member 8 (SLC2A8)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GLUT8
• Synonyms: GLUT8; GLUTX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204317 representing NM_014580; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACGCCCGAGGACCCAGAGGAAACCCAGCCGCTTCTGGGGCCTCCTGGCGGCAGCGCGCCCCGCGGCC; GCCGCGTCTTCCTCGCCGCCTTCGCCGCTGCCCTGGGCCCACTCAGCTTCGGCTTCGCGCTCGGCTACAG; CTCCCCGGCCATCCCTAGCCTGCAGCGCGCCGCGCCCCCGGCCCCGCGCCTGGACGACGCCGCCGCCTCC; TGGTTCGGGGCTGTCGTGACCCTGGGTGCCGCGGCGGGGGGAGTGCTGGGCGGCTGGCTGGTGGACCGCG; CCGGGCGCAAGCTGAGCCTCTTGCTGTGCTCCGTGCCCTTCGTGGCCGGCTTTGCCGTCATCACCGCGGC; CCAGGACGTGTGGATGCTGCTGGGGGGCCGCCTCCTCACCGGCCTGGCCTGCGGTGTTGCCTCCCTAGTG; GCCCCGGTCTACATCTCCGAAATCGCCTACCCAGCAGTCCGGGGGTTGCTCGGCTCCTGTGTGCAGCTAA; TGGTCGTCGTCGGCATCCTCCTGGCCTACCTGGCAGGCTGGGTGCTGGAGTGGCGCTGGCTGGCTGTGCT; GGGCTGCGTGCCCCCCTCCCTCATGCTGCTTCTCATGTGCTTCATGCCCGAGACCCCGCGCTTCCTGCTG; ACTCAGCACAGGCGCCAGGAGGCCATGGCCGCCCTGCGGTTCCTGTGGGGCTCCGAGCAGGGCTGGGAAG; ACCCCCCCATCGGGGCTGAGCAGAGCTTTCACCTGGCCCTGCTGCGGCAGCCCGGCATCTACAAGCCCTT; CATCATCGGCGTCTCCCTGATGGCCTTCCAGCAGCTGTCGGGGGTCAACGCCGTCATGTTCTATGCAGAG; ACCATCTTTGAAGAGGCCAAGTTCAAGGACAGCAGCCTGGCCTCGGTCGTCGTGGGTGTCATCCAGGTGC; TGTTCACAGCTGTGGCGGCTCTCATCATGGACAGAGCAGGGCGGAGGCTGCTCCTGGTCTTGTCAGGTGT; GGTCATGGTGTTCAGCACGAGTGCCTTCGGCGCCTACTTCAAGCTGACCCAGGGTGGCCCTGGCAACTCC; TCGCACGTGGCCATCTCGGCGCCTGTCTCTGCACAGCCTGTTGATGCCAGCGTGGGGCTGGCCTGGCTGG; CCGTGGGCAGCATGTGCCTCTTCATCGCCGGCTTTGCGGTGGGCTGGGGGCCCATCCCCTGGCTCCTCAT; GTCAGAGATCTTCCCTCTGCATGTCAAGGGCGTGGCGACAGGCATCTGCGTCCTCACCAACTGGCTCATG; GCCTTTCTCGTGACCAAGGAGTTCAGCAGCCTCATGGAGGTCCTCAGGCCCTATGGAGCCTTCTGGCTTG; CCTCCGCTTTCTGCATCTTCAGTGTCCTTTTCACTTTGTTCTGTGTCCCTGAAACTAAAGGAAAGACTCT; GGAACAAATCACAGCCCATTTTGAGGGGCGA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204317 representing NM_014580; Red=Cloning site Green=Tags(s); ; MTPEDPEETQPLLGPPGGSAPRGRRVFLAAFAAALGPLSFGFALGYSSPAIPSLQRAAPPAPRLDDAAAS; WFGAVVTLGAAAGGVLGGWLVDRAGRKLSLLLCSVPFVAGFAVITAAQDVWMLLGGRLLTGLACGVASLV; APVYISEIAYPAVRGLLGSCVQLMVVVGILLAYLAGWVLEWRWLAVLGCVPPSLMLLLMCFMPETPRFLL; TQHRRQEAMAALRFLWGSEQGWEDPPIGAEQSFHLALLRQPGIYKPFIIGVSLMAFQQLSGVNAVMFYAE; TIFEEAKFKDSSLASVVVGVIQVLFTAVAALIMDRAGRRLLLVLSGVVMVFSTSAFGAYFKLTQGGPGNS; SHVAISAPVSAQPVDASVGLAWLAVGSMCLFIAGFAVGWGPIPWLLMSEIFPLHVKGVATGICVLTNWLM; AFLVTKEFSSLMEVLRPYGAFWLASAFCIFSVLFTLFCVPETKGKTLEQITAHFEGR; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014580
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014580.5
• RefSeq Size: 2145 bp
• RefSeq ORF: 1434 bp
• Locus ID: 29988
• UniProt ID: Q9NY64
• Cytogenetics: 9q33.3
• Protein Families: Transmembrane
• Summary: This gene belongs to the solute carrier 2A family, which includes intracellular glucose transporters. Based on sequence comparison, the glucose transporters are grouped into three classes and this gene is a member of class II. The encoded protein, like other members of the family, contains several conserved residues and motifs and 12 transmembrane domains with both amino and carboxyl ends being on the cytosolic side of the membrane. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]