Cathepsin F (CTSF) (NM_003793) Human Tagged ORF Clone

SKU RG204047

CTSF (tGFP-tagged) - Human cathepsin F (CTSF)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Cathepsin F
• Synonyms: CATSF; CLN13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204047 representing NM_003793; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGCCCTGGCTGCAGCTCCTGTCGCTGCTGGGGCTGCTCCCGGGCGCAGTGGCCGCCCCCGCCCAGC; CCCGAGCCGCCAGCTTTCAGGCCTGGGGGCCGCCGTCCCCGGAGCTGCTGGCGCCCACCCGCTTCGCGCT; GGAGATGTTCAACCGCGGCCGGGCTGCGGGGACGCGGGCCGTGCTGGGCCTTGTGCGCGGCCGCGTCCGC; CGGGCGGGCCAGGGGTCGCTGTACTCCCTGGAGGCCACCCTGGAGGAGCCACCCTGCAACGACCCCATGG; TGTGCCGGCTCCCCGTGTCCAAGAAAACCCTGCTCTGCAGCTTCCAAGTCCTGGATGAGCTCGGAAGACA; CGTGCTGCTGCGGAAGGACTGTGGCCCAGTGGACACCAAGGTTCCAGGTGCTGGGGAGCCCAAGTCAGCC; TTCACTCAGGGCTCAGCCATGATTTCTTCTCTGTCCCAAAACCATCCAGACAACAGAAACGAGACTTTCA; GCTCAGTCATTTCCCTGTTGAATGAGGATCCCCTGTCCCAGGACTTGCCTGTGAAGATGGCTTCAATCTT; CAAGAACTTTGTCATTACCTATAACCGGACATATGAGTCAAAGGAAGAAGCCCGGTGGCGCCTGTCCGTC; TTTGTCAATAACATGGTGCGAGCACAGAAGATCCAGGCCCTGGACCGTGGCACAGCTCAGTATGGAGTCA; CCAAGTTCAGTGATCTCACAGAGGAGGAGTTCCGCACTATCTACCTGAATACTCTCCTGAGAAAAGAGCC; TGGCAACAAGATGAAGCAAGCCAAGTCTGTGGGTGACCTCGCCCCACCTGAATGGGACTGGAGGAGTAAG; GGGGCTGTCACAAAAGTCAAAGACCAGGGCATGTGTGGCTCCTGCTGGGCCTTCTCAGTCACAGGCAATG; TGGAGGGCCAGTGGTTTCTCAACCAGGGGACCCTGCTCTCCCTCTCTGAACAGGAGCTCTTGGACTGTGA; CAAGATGGACAAGGCCTGCATGGGCGGCTTGCCCTCCAATGCCTACTCGGCCATAAAGAATTTGGGAGGG; CTGGAGACAGAGGATGACTACAGCTACCAGGGTCACATGCAGTCCTGCAACTTCTCAGCAGAGAAGGCCA; AGGTCTACATCAATGACTCCGTGGAGCTGAGCCAGAACGAGCAGAAGCTGGCAGCCTGGCTGGCCAAGAG; AGGCCCAATCTCCGTGGCCATCAATGCCTTTGGCATGCAGTTTTACCGCCACGGGATCTCCCGCCCTCTC; CGGCCCCTCTGCAGCCCTTGGCTCATTGACCATGCGGTGTTGCTTGTGGGCTACGGCAACCGCTCTGACG; TTCCCTTTTGGGCCATCAAGAACAGCTGGGGCACTGACTGGGGTGAGAAGGGTTACTACTACTTGCATCG; CGGGTCCGGGGCCTGTGGCGTGAACACCATGGCCAGCTCGGCGGTGGTGGAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204047 representing NM_003793; Red=Cloning site Green=Tags(s); ; MAPWLQLLSLLGLLPGAVAAPAQPRAASFQAWGPPSPELLAPTRFALEMFNRGRAAGTRAVLGLVRGRVR; RAGQGSLYSLEATLEEPPCNDPMVCRLPVSKKTLLCSFQVLDELGRHVLLRKDCGPVDTKVPGAGEPKSA; FTQGSAMISSLSQNHPDNRNETFSSVISLLNEDPLSQDLPVKMASIFKNFVITYNRTYESKEEARWRLSV; FVNNMVRAQKIQALDRGTAQYGVTKFSDLTEEEFRTIYLNTLLRKEPGNKMKQAKSVGDLAPPEWDWRSK; GAVTKVKDQGMCGSCWAFSVTGNVEGQWFLNQGTLLSLSEQELLDCDKMDKACMGGLPSNAYSAIKNLGG; LETEDDYSYQGHMQSCNFSAEKAKVYINDSVELSQNEQKLAAWLAKRGPISVAINAFGMQFYRHGISRPL; RPLCSPWLIDHAVLLVGYGNRSDVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASSAVVD; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003793
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003793.3, NP_003784.2
• RefSeq Size: 2032 bp
• RefSeq ORF: 1455 bp
• Locus ID: 8722
• UniProt ID: Q9UBX1
• Cytogenetics: 11q13.2
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Summary: Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008]