Claudin 10 (CLDN10) (NM_006984) Human Tagged ORF Clone

SKU RG203090

CLDN10 (tGFP-tagged) - Human claudin 10 (CLDN10), transcript variant b

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Claudin 10
• Synonyms: CPETRL3; HELIX; OSP-L; OSPL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203090 representing NM_006984; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTAGCACGGCTTCGGAGATCATCGCCTTCATGGTCTCCATCTCAGGCTGGGTACTGGTGTCCTCCA; CGCTGCCCACCGACTACTGGAAGGTGTCTACCATCGACGGCACGGTCATCACAACCGCCACCTATTGGGC; CAACCTGTGGAAGGCGTGCGTTACCGACTCCACGGGCGTCTCCAACTGCAAGGACTTCCCCTCCATGCTG; GCGCTGGACGGTTATATACAGGCATGTAGAGGACTTATGATCGCTGCTGTCAGCCTGGGCTTCTTTGGTT; CCATATTTGCGCTCTTTGGAATGAAGTGTACCAAAGTCGGAGGCTCCGATAAAGCCAAAGCTAAAATTGC; TTGTTTGGCTGGGATTGTATTCATACTGTCAGGGCTGTGCTCAATGACTGGATGTTCCCTATATGCAAAC; AAAATCACAACGGAATTCTTTGATCCTCTCTTTGTTGAGCAAAAGTATGAATTAGGAGCCGCTCTGTTTA; TTGGATGGGCAGGAGCCTCACTGTGCATAATTGGTGGTGTCATATTTTGCTTTTCAATATCTGACAACAA; CAAAACACCCAGATACACATACAACGGGGCCACATCTGTCATGTCTTCTCGGACAAAGTATCATGGTGGA; GAAGATTTTAAAACAACAAACCCTTCAAAACAGTTTGATAAAAATGCTTATGTC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203090 representing NM_006984; Red=Cloning site Green=Tags(s); ; MASTASEIIAFMVSISGWVLVSSTLPTDYWKVSTIDGTVITTATYWANLWKACVTDSTGVSNCKDFPSML; ALDGYIQACRGLMIAAVSLGFFGSIFALFGMKCTKVGGSDKAKAKIACLAGIVFILSGLCSMTGCSLYAN; KITTEFFDPLFVEQKYELGAALFIGWAGASLCIIGGVIFCFSISDNNKTPRYTYNGATSVMSSRTKYHGG; EDFKTTNPSKQFDKNAYV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006984
• ORF Size: 684 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006984.5
• RefSeq Size: 2373 bp
• RefSeq ORF: 687 bp
• Locus ID: 9071
• UniProt ID: P78369
• Cytogenetics: 13q32.1
• Domains: PMP22_Claudin
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction
• Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The expression level of this gene is associated with recurrence of primary hepatocellular carcinoma. Six alternatively spliced transcript variants encoding different isoforms have been reported, but the transcript sequences of some variants are not determined.[provided by RefSeq, Jun 2010]