Calpain 10 (CAPN10) (NM_023083) Human Tagged ORF Clone

SKU RG202556

CAPN10 (tGFP-tagged) - Human calpain 10 (CAPN10), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Calpain 10
• Synonyms: CANP10; NIDDM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202556 representing NM_023083; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCGGGCGGGCCGGGGCGCGACGCCGGCGAGGGAGCTGTTCCGGGACGCCGCCTTCCCCGCCGCGGACT; CCTCGCTCTTCTGCGACTTGTCTACGCCGCTGGCCCAGTTCCGCGAGGACATCACGTGGAGGCGGCCCCA; GGAGATTTGTGCCACACCCCGGCTGTTTCCAGATGACCCACGGGAAGGGCAGGTGAAGCAGGGGCTGCTG; GGGGATTGCTGGTTCCTGTGTGCCTGCGCCGCGCTGCAGAAGAGCAGGCACCTCCTGGACCAGGTCATTC; CTCCGGGACAGCCGAGCTGGGCCGACCAGGAGTACCGGGGCTCCTTCACCTGTCGCATTTGGCAGTTTGG; ACGCTGGGTGGAGGTGACCACAGATGACCGCCTGCCGTGCCTTGCAGGGAGACTCTGTTTCTCCCGCTGC; CAGAGGGAGGATGTGTTCTGGCTCCCCTTACTGGAAAAGGTCTACGCCAAGGTCCATGGGTCCTACGAGC; ACCTGTGGGCCGGGCAGGTGGCGGATGCCCTGGTGGACCTGACCGGCGGCCTGGCAGAAAGATGGAACCT; GAAGGGCGTAGCAGGAAGCGGAGGCCAGCAGGACAGGCCAGGCCGCTGGGAGCACAGGACTTGTCGGCAG; CTGCTCCACCTGAAGGACCAGTGTCTGATCAGCTGCTGCGTGCTCAGCCCCAGAGCAGGTGCCCGGGAGC; TGGGGGAGTTCCATGCCTTCATTGTCTCGGACCTGCGGGAGCTCCAGGGTCAGGCGGGCCAGTGCATCCT; GCTGCTGCGGATCCAGAACCCCTGGGGCCGGCGGTGCTGGCAGGGGCTCTGGAGAGAGGGGGGTGAAGGG; TGGAGCCAGGTAGATGCAGCGGTAGCATCTGAGCTCCTGTCCCAGCTCCAGGAAGGGGAGTTCTGGGTGG; AGGAGGAGGAGTTCCTCAGGGAGTTTGACGAGCTCACCGTTGGCTACCCGGTCACGGAGGCCGGCCACCT; GCAGAGCCTCTACACAGAGAGGCTGCTCTGCCATACGCGGGCGCTGCCTGGGGCCTGGGTCAAGGGCCAG; TCAGCAGGAGGCTGCCGGAACAACAGCGGCTTTCCCAGCAACCCCAAATTCTGGCTGCGGGTCTCAGAAC; CGAGTGAGGTGTACATTGCCGTCCTGCAGAGATCCAGGCTGCACGCGGCGGACTGGGCAGGCCGGGCCCG; GGCACTGGTGGGTGACAGTCATACTTCGTGGAGCCCAGCGAGCATCCCGGGCAAGCACTACCAGGCTGTG; GGTCTGCACCTCTGGAAGGTAGAGAAGCGGCGGGTCAATCTGCCTAGGGTCCTGTCCATGCCCCCCGTGG; CTGGCACCGCGTGCCATGCATACGACCGGGAGGTCCACCTGCGTTGTGAGCTCTCACCGGGCTACTACCT; GGCTGTCCCCAGCACCTTCCTGAAGGACGCGCCAGGGGAGTTCCTGCTCCGAGTCTTCTCTACCGGGCGA; GTCTCCCTTAGCGCCATCAGGGCAGTGGCCAAGAACACCACCCCCGGGGCAGCCCTGCCTGCGGGGGAGT; GGGGGACCGTGCAGCTACGGGGTTCTTGGAGAGTCGGCCAGACGGCGGGGGGCAGCAGGAACTTTGCCTC; ATACCCCACCAACCCCTGCTTCCCCTTCTCGGTCCCCGAGGGCCCTGGCCCCCGCTGCGTCCGCATCACT; CTGCATCAGCACTGCCGGCCCAGTGACACCGAGTTCCACCCCATCGGCTTCCATATCTTCCAGGTCCCAG; AGGGTGGAAGGAGCCAGGACGCACCCCCACTGCTGCTGCAGGAGCCGCTGCTGAGCTGCGTGCCACATCG; CTACGCCCAGGAGGTGAGCCGGCTCTGCCTCCTGCCTGCAGGCACCTACAAGGTTGTGCCCTCCACCTAC; CTGCCGGACACAGAGGGGGCCTTCACAGTGACCATCGCAACCAGGATTGACAGGCCATCCATTCACAGCC; AGGAGATGCTGGGCCAGTTCCTCCAAGAGGTCTCCGTCATGGCAGTGATGAAAACC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202556 representing NM_023083; Red=Cloning site Green=Tags(s); ; MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLL; GDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRC; QREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQ; LLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQCILLLRIQNPWGRRCWQGLWREGGEG; WSQVDAAVASELLSQLQEGEFWVEEEEFLREFDELTVGYPVTEAGHLQSLYTERLLCHTRALPGAWVKGQ; SAGGCRNNSGFPSNPKFWLRVSEPSEVYIAVLQRSRLHAADWAGRARALVGDSHTSWSPASIPGKHYQAV; GLHLWKVEKRRVNLPRVLSMPPVAGTACHAYDREVHLRCELSPGYYLAVPSTFLKDAPGEFLLRVFSTGR; VSLSAIRAVAKNTTPGAALPAGEWGTVQLRGSWRVGQTAGGSRNFASYPTNPCFPFSVPEGPGPRCVRIT; LHQHCRPSDTEFHPIGFHIFQVPEGGRSQDAPPLLLQEPLLSCVPHRYAQEVSRLCLLPAGTYKVVPSTY; LPDTEGAFTVTIATRIDRPSIHSQEMLGQFLQEVSVMAVMKT; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023083
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_023083.1, NP_075571.1
• RefSeq Size: 2626 bp
• RefSeq ORF: 2019 bp
• Locus ID: 11132
• UniProt ID: Q9HC96
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, Protease
• Summary: Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]