ZNF691 (NM_015911) Human Tagged ORF Clone

SKU
RG201673
ZNF691 (tGFP-tagged) - Human zinc finger protein 691 (ZNF691)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $500.00 MSRP $500.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF691
Synonyms Zfp691
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201673 representing NM_015911
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGTGAGAAGGAGCAGAGTCCAGAACCACACCTGCCTGAGGAAGGGGAAGGGGGTAAGCCTTGGA
GAGTGGATGACTCAGAGGGTTCTTGGATCCCACCTGGGGAGAAGGAGCATGGGCAAGAGAGCCTGTCGGA
TGAACTGCAAGAAACTCATCCAAAAAAGCCATGGCAGAAAGTCACTGTCCGGGCTCGAGAGCTAGGGGAC
CCCATTGCTCATCCAAGGCATGAGGCAGATGAGAAGCCCTTTATATGTGCCCAGTGTGGCAAAACCTTCA
ATAATACCTCCAACCTGAGAACACACCAGCGGATCCACACTGGTGAGAAGCCTTACAAGTGTTCTGAATG
TGGCAAGAGCTTCTCGAGAAGCTCCAACCGCATCCGGCACGAGCGGATCCACCTGGAAGAGAAACACTAC
AAATGCCCCAAGTGCCAGGAGAGCTTTCGGCGGCGCTCAGACCTCACCACGCACCAGCAAGATCACCTAG
GCAAGCGGCCATACCGCTGTGACATCTGTGGCAAGAGCTTCAGCCAGAGTGCCACGCTAGCTGTGCATCA
CCGGACCCACCTGGAGCCAGCACCCTACATCTGCTGTGAGTGTGGGAAGAGCTTCAGCAACAGCTCCAGC
TTTGGCGTGCATCACCGCACCCACACAGGTGAGAGACCTTATGAGTGCACTGAGTGTGGGCGGACCTTCA
GCGATATCTCCAACTTTGGAGCACACCAGCGGACCCACAGAGGGGAGAAGCCCTACCGGTGCACTGTGTG
TGGGAAACACTTCTCCCGGAGCTCGAATCTCATCCGCCACCAGAAAACTCACTTGGGCGAACAGGCTGGG
AAAGATTCCAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201673 representing NM_015911
Red=Cloning site Green=Tags(s)

MGSEKEQSPEPHLPEEGEGGKPWRVDDSEGSWIPPGEKEHGQESLSDELQETHPKKPWQKVTVRARELGD
PIAHPRHEADEKPFICAQCGKTFNNTSNLRTHQRIHTGEKPYKCSECGKSFSRSSNRIRHERIHLEEKHY
KCPKCQESFRRRSDLTTHQQDHLGKRPYRCDICGKSFSQSATLAVHHRTHLEPAPYICCECGKSFSNSSS
FGVHHRTHTGERPYECTECGRTFSDISNFGAHQRTHRGEKPYRCTVCGKHFSRSSNLIRHQKTHLGEQAG
KDSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_015911
ORF Size 852 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015911.3
RefSeq Size 1591 bp
RefSeq ORF 855 bp
Locus ID 51058
UniProt ID Q5VV52
Cytogenetics 1p34.2
Domains zf-C2H2
Summary May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]
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Citations

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