Cip4 (TRIP10) (NM_004240) Human Tagged ORF Clone

SKU RG201200

TRIP10 (tGFP-tagged) - Human thyroid hormone receptor interactor 10 (TRIP10)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Cip4
• Synonyms: CIP4; HSTP; STOT; STP; TRIP-10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201200 representing NM_004240; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGATTGGGGCACTGAGCTGTGGGATCAGTTCGAGGTGCTCGAGCGCCACACGCAGTGGGGGCTGGACC; TGTTGGACAGATATGTAAAGTTCGTGAAAGAACGCACCGAAGTGGAACAGGCTTACGCCAAACAACTGCG; GAGCCTGGTGAAAAAATATCTGCCCAAGAGACCTGCCAAGGATGATCCTGAGTCCAAATTCAGCCAGCAA; CAGTCCTTCGTACAGATTCTCCAGGAGGTGAATGACTTTGCAGGCCAGCGGGAGCTGGTGGCTGAGAACC; TCAGTGTCCGTGTATGTCTTGAGCTGACCAAGTACTCACAAGAGATGAAACAGGAGAGGAAGATGCACTT; CCAAGAAGGGCGGCGGGCCCAGCAGCAGCTGGAAAATGGCTTTAAACAGCTGGAGAATAGTAAGCGTAAA; TTTGAGCGGGACTGCCGGGAGGCAGAGAAGGCAGCCCAGACTGCTGAACGGCTAGACCAGGATATCAACG; CCACCAAGGCTGATGTGGAGAAGGCCAAGCAGCAAGCCCACCTTCGGAGTCACATGGCCGAAGAAAGCAA; AAACGAATATGCGGCTCAACTGCAGCGCTTCAACCGAGACCAAGCCCACTTCTATTTTTCACAGATGCCC; CAGATATTCGATAAGCTCCAAGACATGGATGAACGCAGGGCCACCCGCCTGGGTGCCGGGTATGGGCTCC; TGTCGGAGGCCGAGCTGGAGGTGGTGCCCATAATAGCCAAGTGCTTGGAGGGCATGAAGGTGGCTGCAAA; TGCTGTGGATCCCAAGAACGACTCCCACGTCCTTATAGAGCTGCACAAGTCAGGTTTTGCCCGCCCGGGC; GACGTGGAATTCGAGGACTTCAGCCAGCCCATGAACCGTGCACCCTCCGACAGCAGTCTGGGCACCCCCT; CGGATGGACGGCCTGAACTCCGAGGCCCGGGTCGCAGCCGCACCAAGCGCTGGCCTTTTGGCAAGAAGAA; CAAGACAGTGGTGACCGAGGATTTTAGCCACTTGCCCCCAGAGCAGCAGCGAAAACGGCTTCAACAGCAG; TTGGAAGAACGCAGTCGTGAACTTCAGAAGGAGGTTGACCAGAGGGAAGCCCTAAAGAAAATGAAGGATG; TCTATGAGAAGACACCTCAGATGGGGGACCCCGCCAGCTTGGAGCCCCAGATCGCTGAAACCCTGAGCAA; CATTGAACGGCTGAAATTGGAAGTGCAGAAGTATGAGGCGTGGCTGGCAGAAGCTGAAAGTCGAGTCCTT; AGCAACCGGGGAGACAGCCTGAGCCGGCACGCCCGGCCTCCCGACCCCCCCGCTAGCGCCCCGCCAGACA; GCAGCAGCAACAGCGCATCACAGGACACCAAGGAGAGCTCTGAAGAGCCTCCCTCAGAAGAGAGCCAGGA; CACCCCCATTTACACGGAGTTTGATGAGGATTTCGAGGAGGAACCCACATCCCCCATAGGTCACTGTGTG; GCCATCTACCACTTTGAAGGGTCCAGCGAGGGCACTATCTCTATGGCCGAGGGTGAAGACCTCAGTCTTA; TGGAAGAAGACAAAGGGGACGGCTGGACCCGGGTCAGGCGGAAAGAGGGAGGCGAGGGCTACGTGCCCAC; CTCCTACCTCCGAGTCACGCTCAAT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201200 representing NM_004240; Red=Cloning site Green=Tags(s); ; MDWGTELWDQFEVLERHTQWGLDLLDRYVKFVKERTEVEQAYAKQLRSLVKKYLPKRPAKDDPESKFSQQ; QSFVQILQEVNDFAGQRELVAENLSVRVCLELTKYSQEMKQERKMHFQEGRRAQQQLENGFKQLENSKRK; FERDCREAEKAAQTAERLDQDINATKADVEKAKQQAHLRSHMAEESKNEYAAQLQRFNRDQAHFYFSQMP; QIFDKLQDMDERRATRLGAGYGLLSEAELEVVPIIAKCLEGMKVAANAVDPKNDSHVLIELHKSGFARPG; DVEFEDFSQPMNRAPSDSSLGTPSDGRPELRGPGRSRTKRWPFGKKNKTVVTEDFSHLPPEQQRKRLQQQ; LEERSRELQKEVDQREALKKMKDVYEKTPQMGDPASLEPQIAETLSNIERLKLEVQKYEAWLAEAESRVL; SNRGDSLSRHARPPDPPASAPPDSSSNSASQDTKESSEEPPSEESQDTPIYTEFDEDFEEEPTSPIGHCV; AIYHFEGSSEGTISMAEGEDLSLMEEDKGDGWTRVRRKEGGEGYVPTSYLRVTLN; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004240
• ORF Size: 1635 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004240.4
• RefSeq Size: 2029 bp
• RefSeq ORF: 1638 bp
• Locus ID: 9322
• UniProt ID: Q15642
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• Summary: Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.[UniProtKB/Swiss-Prot Function]