MXRA8 (NM_032348) Human Tagged ORF Clone

SKU RG200955

MXRA8 (tGFP-tagged) - Human matrix-remodelling associated 8 (MXRA8)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MXRA8
• Synonyms: ASP3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200955 representing NM_032348; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGCTGCCATCCCGAATCCTGCTTTGGAAACTTGTGCTTCTGCAGAGCTCTGCTGTTCTCCTGCACT; CAGGGTCCTCGGTACCCGCCGCTGCTGGCAGCTCCGTGGTGTCCGAGTCCGCGGTGAGCTGGGAGGCGGG; CGCCCGGGCGGTGCTGCGCTGCCAGAGCCCGCGCATGGTGTGGACCCAGGACCGGCTGCACGACCGCCAG; CGCGTGCTCCACTGGGACCTGCGCGGCCCCGGGGGTGGCCCCGCGCGGCGCCTGCTGGACTTGTACTCGG; CGGGCGAGCAGCGCGTGTACGAGGCGCGGGACCGCGGCCGCCTGGAGCTCTCGGCCTCGGCCTTCGACGA; CGGCAACTTCTCGCTGCTCATCCGCGCGGTGGAGGAGACGGACGCGGGGCTGTACACCTGCAACCTGCAC; CATCACTACTGCCACCTCTACGAGAGCCTGGCCGTCCGCCTGGAGGTCACCGACGGCCCCCCGGCCACCC; CCGCCTACTGGGACGGCGAGAAGGAGGTGCTGGCGGTGGCGCGCGGCGCACCCGCGCTTCTGACCTGCGT; GAACCGCGGGCACGTGTGGACCGACCGGCACGTGGAGGAGGCTCAACAGGTGGTGCACTGGGACCGGCAG; CCGCCCGGGGTCCCGCACGACCGCGCGGACCGCCTGCTGGACCTCTACGCGTCGGGCGAGCGCCGCGCCT; ACGGGCCCCTTTTTCTGCGCGACCGCGTGGCTGTGGGCGCGGATGCCTTTGAGCGCGGTGACTTCTCACT; GCGTATCGAGCCGCTGGAGGTCGCCGACGAGGGCACCTACTCCTGCCACCTGCACCACCATTACTGTGGC; CTGCACGAACGCCGCGTCTTCCACCTGACGGTCGCCGAACCCCACGCGGAGCCGCCCCCCCGGGGCTCTC; CGGGCAACGGCTCCAGCCACAGCGGCGCCCCAGGCCCAGACCCCACACTGGCGCGCGGCCACAACGTCAT; CAATGTCATCGTCCCCGAGAGCCGAGCCCACTTCTTCCAGCAGCTGGGCTACGTGCTGGCCACGCTGCTG; CTCTTCATCCTGCTACTGGTCACTGTCCTCCTGGCCGCCCGCAGGCGCCGCGGAGGCTACGAATACTCGG; ACCAGAAGTCGGGAAAGTCAAAGGGGAAGGATGTTAACTTGGCGGAGTTCGCTGTGGCTGCAGGGGACCA; GATGCTTTACAGGAGTGAGGACATCCAGCTAGATTACAAAAACAACATCCTGAAGGAGAGGGCGGAGCTG; GCCCACAGCCCCCTGCCTGCCAAGTACATCGACCTAGACAAAGGGTTCCGGAAGGAGAACTGCAAA; ; CTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200955 representing NM_032348; Red=Cloning site Green=Tags(s); ; MALPSRILLWKLVLLQSSAVLLHSGSSVPAAAGSSVVSESAVSWEAGARAVLRCQSPRMVWTQDRLHDRQ; RVLHWDLRGPGGGPARRLLDLYSAGEQRVYEARDRGRLELSASAFDDGNFSLLIRAVEETDAGLYTCNLH; HHYCHLYESLAVRLEVTDGPPATPAYWDGEKEVLAVARGAPALLTCVNRGHVWTDRHVEEAQQVVHWDRQ; PPGVPHDRADRLLDLYASGERRAYGPLFLRDRVAVGADAFERGDFSLRIEPLEVADEGTYSCHLHHHYCG; LHERRVFHLTVAEPHAEPPPRGSPGNGSSHSGAPGPDPTLARGHNVINVIVPESRAHFFQQLGYVLATLL; LFILLLVTVLLAARRRRGGYEYSDQKSGKSKGKDVNLAEFAVAAGDQMLYRSEDIQLDYKNNILKERAEL; AHSPLPAKYIDLDKGFRKENCK; ; LE - GFP Tag - V
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032348
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032348.4
• RefSeq Size: 2290 bp
• RefSeq ORF: 1329 bp
• Locus ID: 54587
• UniProt ID: Q9BRK3
• Cytogenetics: 1p36.33
• Protein Families: Transmembrane
• Summary: Transmembrane protein which can modulate activity of various signaling pathways, probably via binding to integrin ITGAV:ITGB3 (PubMed:22492581, PubMed:23386276). Mediates heterophilic cell-cell interactions in vitro (By similarity). Inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1, where it may function by attenuating signaling via integrin ITGB3 and MAP kinase p38 (By similarity). Plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes (By similarity). Stimulates formation of primary cilia in chondrocytes (By similarity). Enhances expression of genes involved in the hedgehog signaling pathway in chondrocytes, including the hedgehog signaling molecule IHH; may also promote signaling via the PTHLH/PTHrP pathway (By similarity). Plays a role in angiogenesis where it suppresses migration of endothelial cells and also promotes their apoptosis (PubMed:23386276). Inhibits VEGF-induced activation of AKT and p38 MAP kinase in endothelial cells (PubMed:23386276). Also inhibits VTN (vitronectin)-mediated integrin ITGAV:ITGB3 signaling and activation of PTK2/FAK (PubMed:23386276). May play a role in the maturation and maintenance of the blood-brain barrier (By similarity).[UniProtKB/Swiss-Prot Function]