MDS028 (ITFG2) (NM_018463) Human Tagged ORF Clone

SKU RG200818

ITFG2 (tGFP-tagged) - Human integrin alpha FG-GAP repeat containing 2 (ITFG2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MDS028
• Synonyms: FGGAP1; MDS028
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200818 representing NM_018463; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGTCAGTTAGCTACGTGCAGCGCGTGGCGCTGGAGTTCAGCGGGAGCCTCTTCCCGCACGCAATCT; GCCTCGGAGACGTTGATAACGATACGTTAAATGAACTGGTGGTGGGAGACACCAGCGGGAAGGTGTCTGT; GTATAAAAATGATGACAGTCGGCCATGGCTCACCTGTTCCTGCCAGGGAATGCTGACTTGCGTTGGGGTT; GGAGACGTGTGTAATAAAGGAAAGAACCTGTTGGTGGCAGTGAGTGCTGAAGGCTGGTTTCATTTGTTTG; ACCTGACACCTGCCAAGGTGTTGGATGCTTCTGGGCACCACGAGACACTAATCGGAGAGGAGCAGCGTCC; AGTCTTCAAGCAGCACATCCCTGCCAACACCAAGGTCATGCTGATCAGCGACATCGATGGAGATGGGTGT; CGTGAGCTGGTGGTGGGCTACACAGACCGTGTGGTGCGAGCTTTCCGCTGGGAGGAGCTAGGTGAGGGTC; CTGAACATCTGACAGGGCAGCTGGTGTCCCTCAAGAAATGGATGCTGGAGGGTCAGGTGGACAGCCTCTC; AGTGACTCTGGGGCCACTGGGTCTTCCTGAACTGATGGTGTCTCAGCCAGGTTGTGCGTATGCAATTCTA; CTGTGTACCTGGAAAAAGGACACTGGGTCCCCTCCTGCCTCTGAAGGGCCCACGGATGGTAGTAGGGAGA; CCCCAGCTGCCCGAGACGTGGTGCTGCACCAGACATCTGGCCGTATCCACAACAAGAATGTCTCCACTCA; CCTAATTGGCAACATCAAACAAGGCCACGGCACTGAGAGTAGTGGCTCTGGCCTCTTTGCCCTGTGCACC; CTGGATGGGACACTGAAGCTCATGGAAGAAATGGAAGAAGCAGACAAGCTGCTGTGGTCAGTGCAGGTGG; ATCACCAGCTCTTTGCCCTGGAGAAACTGGATGTCACCGGCAACGGGCATGAGGAGGTAGTTGCATGCGC; CTGGGATGGACAGACATATATCATTGATCACAACCGCACCGTCGTCCGCTTCCAAGTGGATGAAAATATC; CGTGCCTTCTGTGCAGGCCTGTACGCCTGCAAAGAGGGCCGCAACAGCCCCTGCCTCGTATATGTCACTT; TCAACCAGAAGATCTATGTGTACTGGGAGGTGCAGCTGGAGCGGATGGAGTCTACCAATCTGGTGAAACT; GCTGGAGACCAAGCCGGAGTACCACAGCCTGCTGCAGGAGCTGGGCGTGGATCCTGACGACCTCCCTGTG; ACTCGTGCCCTGCTTCACCAAACGCTCTACCATCCAGACCAGCCACCACAGTGTGCTCCCTCAAGCCTCC; AGGATCCCACC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200818 representing NM_018463; Red=Cloning site Green=Tags(s); ; MRSVSYVQRVALEFSGSLFPHAICLGDVDNDTLNELVVGDTSGKVSVYKNDDSRPWLTCSCQGMLTCVGV; GDVCNKGKNLLVAVSAEGWFHLFDLTPAKVLDASGHHETLIGEEQRPVFKQHIPANTKVMLISDIDGDGC; RELVVGYTDRVVRAFRWEELGEGPEHLTGQLVSLKKWMLEGQVDSLSVTLGPLGLPELMVSQPGCAYAIL; LCTWKKDTGSPPASEGPTDGSRETPAARDVVLHQTSGRIHNKNVSTHLIGNIKQGHGTESSGSGLFALCT; LDGTLKLMEEMEEADKLLWSVQVDHQLFALEKLDVTGNGHEEVVACAWDGQTYIIDHNRTVVRFQVDENI; RAFCAGLYACKEGRNSPCLVYVTFNQKIYVYWEVQLERMESTNLVKLLETKPEYHSLLQELGVDPDDLPV; TRALLHQTLYHPDQPPQCAPSSLQDPT; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018463
• ORF Size: 1341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018463.4
• RefSeq Size: 2155 bp
• RefSeq ORF: 1344 bp
• Locus ID: 55846
• UniProt ID: Q969R8
• Cytogenetics: 12p13.33
• Domains: FG-GAP
• Protein Families: Druggable Genome
• Summary: As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose.[UniProtKB/Swiss-Prot Function]