HYAL2 (NM_033158) Human Tagged ORF Clone

SKU RG200552

HYAL2 (tGFP-tagged) - Human hyaluronoglucosaminidase 2 (HYAL2), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: HYAL2
• Synonyms: LUCA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200552 representing NM_033158; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCGGGCAGGCCCAGGCCCCACCGTTACATTGGCCCTGGTGCTGGCGGTGGCATGGGCCATGGAGCTCA; AGCCCACAGCACCACCCATCTTCACTGGCCGGCCCTTTGTGGTAGCGTGGGACGTGCCCACACAGGACTG; TGGCCCACGCCTCAAGGTGCCACTGGACCTGAATGCCTTTGATGTGCAGGCCTCACCTAATGAGGGTTTT; GTGAACCAGAATATTACCATCTTCTACCGCGACCGTCTAGGCCTGTATCCACGCTTCGATTCTGCCGGAA; GGTCTGTGCATGGTGGTGTGCCACAGAATGTCAGCCTTTGGGCACACCGGAAGATGCTGCAGAAACGTGT; GGAGCACTACATTCGGACACAGGAGTCTGCGGGGCTGGCGGTCATCGACTGGGAGGACTGGCGACCTGTG; TGGGTGCGCAACTGGCAGGACAAAGATGTGTATCGCCGGTTATCACGCCAGCTAGTGGCCAGTCGTCACC; CTGACTGGCCTCCAGACCGCATAGTCAAACAGGCACAATATGAGTTTGAGTTCGCAGCACAGCAGTTCAT; GCTGGAGACACTGCGTTATGTCAAGGCAGTGCGGCCCCGGCACCTCTGGGGCTTCTACCTCTTTCCTGAC; TGCTACAATCATGATTATGTGCAGAACTGGGAGAGCTACACAGGCCGCTGCCCTGATGTTGAGGTGGCCC; GCAATGACCAGCTGGCCTGGCTGTGGGCTGAGAGCACGGCCCTCTTCCCGTCTGTCTACCTGGACGAGAC; ACTTGCTTCCTCCCGCCATGGCCGCAACTTTGTGAGCTTCCGTGTTCAGGAGGCCCTTCGTGTGGCTCGC; ACCCACCATGCCAACCATGCACTCCCAGTCTACGTCTTCACACGACCCACCTACAGCCGCAGGCTCACGG; GGCTTAGTGAGATGGACCTCATCTCTACCATTGGCGAGAGTGCGGCCCTGGGCGCAGCTGGTGTCATCCT; CTGGGGTGACGCGGGGTACACCACAAGCACGGAGACCTGCCAGTACCTCAAAGATTACCTGACACGGCTG; CTGGTCCCCTACGTGGTCAATGTGTCCTGGGCCACCCAATATTGCAGCCGGGCCCAGTGCCATGGCCATG; GGCGCTGTGTGCGCCGCAACCCCAGTGCCAGTACCTTCCTGCATCTCAGCACCAACAGTTTCCGCCTAGT; GCCTGGCCATGCACCTGGTGAACCCCAGCTGCGACCTGTGGGGGAGCTCAGTTGGGCCGACATTGACCAC; CTGCAGACACACTTCCGCTGCCAGTGCTACTTGGGCTGGAGTGGTGAGCAATGCCAGTGGGACCATAGGC; AGGCAGCTGGAGGTGCCAGCGAGGCCTGGGCTGGGTCCCACCTCACCAGTCTGCTGGCTCTGGCAGCCCT; GGCCTTTACCTGGACCTTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200552 representing NM_033158; Red=Cloning site Green=Tags(s); ; MRAGPGPTVTLALVLAVAWAMELKPTAPPIFTGRPFVVAWDVPTQDCGPRLKVPLDLNAFDVQASPNEGF; VNQNITIFYRDRLGLYPRFDSAGRSVHGGVPQNVSLWAHRKMLQKRVEHYIRTQESAGLAVIDWEDWRPV; WVRNWQDKDVYRRLSRQLVASRHPDWPPDRIVKQAQYEFEFAAQQFMLETLRYVKAVRPRHLWGFYLFPD; CYNHDYVQNWESYTGRCPDVEVARNDQLAWLWAESTALFPSVYLDETLASSRHGRNFVSFRVQEALRVAR; THHANHALPVYVFTRPTYSRRLTGLSEMDLISTIGESAALGAAGVILWGDAGYTTSTETCQYLKDYLTRL; LVPYVVNVSWATQYCSRAQCHGHGRCVRRNPSASTFLHLSTNSFRLVPGHAPGEPQLRPVGELSWADIDH; LQTHFRCQCYLGWSGEQCQWDHRQAAGGASEAWAGSHLTSLLALAALAFTWTL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033158
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_033158.1
• RefSeq Size: 1909 bp
• RefSeq ORF: 1422 bp
• Locus ID: 8692
• UniProt ID: Q12891
• Cytogenetics: 3p21.31
• Domains: Glyco_hydro_56
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Summary: This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010]