Neurofascin (NFASC) (NM_001160331) Human Tagged ORF Clone

CAT#: RC228652

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NFASC (Myc-DDK-tagged)-Human neurofascin (NFASC), transcript variant 2



  "NM_001160331" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,496.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NFASC
Synonyms NEDCPMD; NF; NRCAML
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC228652 representing NM_001160331
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGGCAGCCACCGCCGCCCTGGGTCCATGCAGCCTTCCTCCTCTGCCTCCTCAGTCTTGGCGGAG
CCATCGAAATTCCTATGGATCTGACGCAGCCGCCAACCATCACCAAGCAGTCAGCGAAGGATCACATCGT
GGACCCCCGTGATAACATCCTGATTGAGTGTGAAGCAAAAGGGAACCCTGCCCCCAGCTTCCACTGGACA
CGAAACAGCAGATTCTTCAACATCGCCAAGGACCCCCGGGTGTCCATGAGGAGGAGGTCTGGGACCCTGG
TGATTGACTTCCGCAGTGGCGGGCGGCCGGAGGAATATGAGGGGGAATATCAGTGCTTCGCCCGCAACAA
ATTTGGCACGGCCCTGTCCAATAGGATCCGCCTGCAGGTGTCTAAATCTCCTCTGTGGCCCAAGGAAAAC
CTAGACCCTGTCGTGGTCCAAGAGGGCGCTCCTTTGACGCTCCAGTGCAACCCCCCGCCTGGACTTCCAT
CCCCGGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAAGACAAACGTGTCTCTCAGGGCCA
TAACGGAGACCTATACTTCTCCAACGTGATGCTGCAGGACATGCAGACCGACTACAGTTGTAACGCCCGC
TTCCACTTCACCCACACCATCCAGCAGAAGAACCCTTTCACCCTCAAGGTCCTCACCAACCACCCTTATA
ATGACTCGTCCTTAAGAAACCACCCTGACATGTACAGTGCCCGAGGAGTTGCAGAAAGAACACCAAGCTT
CATGTATCCCCAGGGCACCGCGAGCAGCCAGATGGTGCTTCGTGGCATGGACCTCCTGCTGGAATGCATC
GCCTCCGGGGTCCCAACACCAGACATCGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGATAAGGCCA
AGTTTGAGAACTTTAATAAGGCCCTGCGTATCACAAATGTCTCTGAGGAAGACTCCGGGGAGTATTTCTG
CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCCTACTGG
CTGGACGAACCCAAGAACCTTATTCTGGCTCCTGGCGAGGATGGGAGACTGGTGTGTCGAGCCAATGGAA
ACCCCAAACCCACTGTCCAGTGGATGGTGAATGGGGAACCTTTGCAATCGGCACCACCTAACCCAAACCG
TGAGGTGGCCGGAGACACCATCATCTTCCGGGACACCCAGATCAGCAGCAGGGCTGTGTACCAGTGCAAC
ACCTCCAACGAGCATGGCTACCTGCTGGCCAACGCCTTTGTCAGTGTGCTGGATGTGCCGCCTCGGATGC
TGTCGCCCCGGAACCAGCTCATTCGAGTGATTCTTTACAACCGGACGCGGCTGGACTGCCCTTTCTTTGG
GTCTCCCATCCCCACACTGCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGCAACTACCAT
GTTTATGAGAACGGCAGTCTGGAAATTAAGATGATCCGCAAAGAGGACCAGGGCATCTACACCTGTGTCG
CCACCAACATCCTGGGCAAAGCTGAAAACCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACCG
GATGCCCGAGGACCAGGTGGCCAGAAGGGGCACCACGGTGCAGCTGGAGTGTCGGGTGAAGCACGACCCC
TCCCTGAAACTCACCGTCTCCTGGCTGAAGGATGACGAGCCGCTCTATATTGGAAACAGGATGAAGAAGG
AAGACGACTCCCTGACCATCTTTGGGGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTGTCGCCAGCAC
CGAGCTAGACCAAGACCTGGCCAAGGCCTACCTCACCGTGCTAGCTGATCAGGCCACTCCAACTAACCGT
TTGGCTGCCCTGCCCAAAGGACGGCCAGACCGGCCCCGGGACCTGGAGCTGACCGACCTGGCCGAGAGGA
GCGTGCGGCTGACCTGGATCCCCGGGGATGCTAACAACAGCCCCATCACAGACTACGTCGTCCAGTTTGA
AGAAGACCAGTTCCAACCTGGGGTCTGGCATGACCATTCCAAGTACCCCGGCAGCGTTAACTCAGCCGTC
CTCCGGCTGTCCCCGTATGTCAACTACCAGTTCCGTGTCATTGCCATCAACGAGGTTGGGAGCAGCCACC
CCAGCCTCCCATCCGAGCGCTACCGAACCAGTGGAGCACCCCCCGAGTCCAATCCTGGTGACGTGAAGGG
AGAGGGGACCAGAAAGAACAACATGGAGATCACGTGGACGCCCATGAATGCCACCTCGGCCTTTGGCCCC
AACCTGCGCTACATTGTCAAGTGGAGGCGGAGAGAGACTCGAGAGGCCTGGAACAACGTCACAGTGTGGG
GCTCTCGCTACGTGGTGGGGCAGACCCCAGTCTACGTGCCCTATGAGATCCGAGTCCAGGCTGAAAATGA
CTTCGGGAAGGGCCCTGAGCCAGAGTCCGTCATCGGTTACTCCGGAGAAGATTATCCCAGGGCTGCGCCC
ACTGAAGTTAAAGTCCGAGTCATGAACAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACTCCGACA
CGGTCCAGGGCCAGCTCAGAGAGTACCGAGCCTACTACTGGAGGGAGAGCAGCTTGCTGAAGAACCTGTG
GGTGTCTCAGAAGAGACAGCAAGCCAGCTTCCCTGGTGACCGCCTCCGTGGCGTGGTGTCCCGCCTCTTC
CCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGCAGTGAGACCA
AGGAGTTCACCACCCCGGAAGGAGTACCCAGTGCCCCTAGGCGTTTCCGAGTCCGGCAGCCCAACCTGGA
GACAATCAACCTGGAATGGGATCATCCTGAGCATCCAAATGGGATCATGATTGGATACACTCTCAAATAT
GTGGCCTTTAACGGGACCAAAGTAGGAAAGCAGATAGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCA
CGGTGCAAAGAACGGACCCCGTGTCACGCTACCGCTTTACCCTCAGCGCCAGGACGCAGGTGGGCTCTGG
GGAAGCCGTCACAGAGGAGTCACCAGCACCCCCGAATGAAGCTACTCCAACCGCAGCTTACACCAACAAC
CAAGCGGACATCGCCACCCAGGGCTGGTTCATTGGGCTTATGTGCGCCATCGCCCTCCTGGTGCTGATCC
TGCTCATCGTCTGTTTCATCAAGAGGAGTCGCGGCGGCAAGTACCCAGTACGAGAAAAGAAGGATGTTCC
CCTTGGCCCTGAAGACCCCAAGGAAGAGGATGGCTCATTTGACTATAGTGATGAGGACAACAAGCCCCTG
CAGGGCAGTCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGCCTGGTGGACTATG
GCGAGGGTGGCGAGGGTCAGTTCAATGAAGACGGCTCCTTCATCGGCCAGTACACGGTCAAAAAGGACAA
GGAGGAAACAGAGGGCAACGAAAGCTCAGAGGCCACGTCACCTGTCAATGCTATCTACTCTCTGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC228652 representing NM_001160331
Red=Cloning site Green=Tags(s)

MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWT
RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
FHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECI
ASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW
LDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN
TSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH
VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDP
SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNR
LAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAV
LRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGP
NLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAP
TEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLF
PYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKY
VAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNN
QADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPL
QGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001160331
ORF Size 3567 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001160331.1, NP_001153803.1
RefSeq ORF 3570 bp
Locus ID 23114
UniProt ID O94856
Cytogenetics 1q32.1
Protein Families Transmembrane
Protein Pathways Cell adhesion molecules (CAMs)
MW 133.66 kDa
Gene Summary This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]

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