Neurofascin (NFASC) (NM_001160331) Human Tagged ORF Clone

CAT#: RG228652

NFASC (tGFP-tagged) - Human neurofascin (NFASC), transcript variant 2

Product Images

Circular map for RG228652

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Neurofascin
• Synonyms: NEDCPMD; NF; NRCAML
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG228652 representing NM_001160331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGCCACCGCCGCCCTGGGTCCATGCAGCCTTCCTCCTCTGCCTCCTCAGTCTTGGCGGAG
  CCATCGAAATTCCTATGGATCTGACGCAGCCGCCAACCATCACCAAGCAGTCAGCGAAGGATCACATCGT
  GGACCCCCGTGATAACATCCTGATTGAGTGTGAAGCAAAAGGGAACCCTGCCCCCAGCTTCCACTGGACA
  CGAAACAGCAGATTCTTCAACATCGCCAAGGACCCCCGGGTGTCCATGAGGAGGAGGTCTGGGACCCTGG
  TGATTGACTTCCGCAGTGGCGGGCGGCCGGAGGAATATGAGGGGGAATATCAGTGCTTCGCCCGCAACAA
  ATTTGGCACGGCCCTGTCCAATAGGATCCGCCTGCAGGTGTCTAAATCTCCTCTGTGGCCCAAGGAAAAC
  CTAGACCCTGTCGTGGTCCAAGAGGGCGCTCCTTTGACGCTCCAGTGCAACCCCCCGCCTGGACTTCCAT
  CCCCGGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAAGACAAACGTGTCTCTCAGGGCCA
  TAACGGAGACCTATACTTCTCCAACGTGATGCTGCAGGACATGCAGACCGACTACAGTTGTAACGCCCGC
  TTCCACTTCACCCACACCATCCAGCAGAAGAACCCTTTCACCCTCAAGGTCCTCACCAACCACCCTTATA
  ATGACTCGTCCTTAAGAAACCACCCTGACATGTACAGTGCCCGAGGAGTTGCAGAAAGAACACCAAGCTT
  CATGTATCCCCAGGGCACCGCGAGCAGCCAGATGGTGCTTCGTGGCATGGACCTCCTGCTGGAATGCATC
  GCCTCCGGGGTCCCAACACCAGACATCGCATGGTACAAGAAAGGTGGGGACCTCCCATCTGATAAGGCCA
  AGTTTGAGAACTTTAATAAGGCCCTGCGTATCACAAATGTCTCTGAGGAAGACTCCGGGGAGTATTTCTG
  CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCCTACTGG
  CTGGACGAACCCAAGAACCTTATTCTGGCTCCTGGCGAGGATGGGAGACTGGTGTGTCGAGCCAATGGAA
  ACCCCAAACCCACTGTCCAGTGGATGGTGAATGGGGAACCTTTGCAATCGGCACCACCTAACCCAAACCG
  TGAGGTGGCCGGAGACACCATCATCTTCCGGGACACCCAGATCAGCAGCAGGGCTGTGTACCAGTGCAAC
  ACCTCCAACGAGCATGGCTACCTGCTGGCCAACGCCTTTGTCAGTGTGCTGGATGTGCCGCCTCGGATGC
  TGTCGCCCCGGAACCAGCTCATTCGAGTGATTCTTTACAACCGGACGCGGCTGGACTGCCCTTTCTTTGG
  GTCTCCCATCCCCACACTGCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGTGGCAACTACCAT
  GTTTATGAGAACGGCAGTCTGGAAATTAAGATGATCCGCAAAGAGGACCAGGGCATCTACACCTGTGTCG
  CCACCAACATCCTGGGCAAAGCTGAAAACCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACCG
  GATGCCCGAGGACCAGGTGGCCAGAAGGGGCACCACGGTGCAGCTGGAGTGTCGGGTGAAGCACGACCCC
  TCCCTGAAACTCACCGTCTCCTGGCTGAAGGATGACGAGCCGCTCTATATTGGAAACAGGATGAAGAAGG
  AAGACGACTCCCTGACCATCTTTGGGGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTGTCGCCAGCAC
  CGAGCTAGACCAAGACCTGGCCAAGGCCTACCTCACCGTGCTAGCTGATCAGGCCACTCCAACTAACCGT
  TTGGCTGCCCTGCCCAAAGGACGGCCAGACCGGCCCCGGGACCTGGAGCTGACCGACCTGGCCGAGAGGA
  GCGTGCGGCTGACCTGGATCCCCGGGGATGCTAACAACAGCCCCATCACAGACTACGTCGTCCAGTTTGA
  AGAAGACCAGTTCCAACCTGGGGTCTGGCATGACCATTCCAAGTACCCCGGCAGCGTTAACTCAGCCGTC
  CTCCGGCTGTCCCCGTATGTCAACTACCAGTTCCGTGTCATTGCCATCAACGAGGTTGGGAGCAGCCACC
  CCAGCCTCCCATCCGAGCGCTACCGAACCAGTGGAGCACCCCCCGAGTCCAATCCTGGTGACGTGAAGGG
  AGAGGGGACCAGAAAGAACAACATGGAGATCACGTGGACGCCCATGAATGCCACCTCGGCCTTTGGCCCC
  AACCTGCGCTACATTGTCAAGTGGAGGCGGAGAGAGACTCGAGAGGCCTGGAACAACGTCACAGTGTGGG
  GCTCTCGCTACGTGGTGGGGCAGACCCCAGTCTACGTGCCCTATGAGATCCGAGTCCAGGCTGAAAATGA
  CTTCGGGAAGGGCCCTGAGCCAGAGTCCGTCATCGGTTACTCCGGAGAAGATTATCCCAGGGCTGCGCCC
  ACTGAAGTTAAAGTCCGAGTCATGAACAGCACAGCCATCAGCCTTCAGTGGAACCGCGTCTACTCCGACA
  CGGTCCAGGGCCAGCTCAGAGAGTACCGAGCCTACTACTGGAGGGAGAGCAGCTTGCTGAAGAACCTGTG
  GGTGTCTCAGAAGAGACAGCAAGCCAGCTTCCCTGGTGACCGCCTCCGTGGCGTGGTGTCCCGCCTCTTC
  CCCTACAGTAACTACAAGCTGGAGATGGTTGTGGTCAATGGGAGAGGTGATGGGCCTCGCAGTGAGACCA
  AGGAGTTCACCACCCCGGAAGGAGTACCCAGTGCCCCTAGGCGTTTCCGAGTCCGGCAGCCCAACCTGGA
  GACAATCAACCTGGAATGGGATCATCCTGAGCATCCAAATGGGATCATGATTGGATACACTCTCAAATAT
  GTGGCCTTTAACGGGACCAAAGTAGGAAAGCAGATAGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCA
  CGGTGCAAAGAACGGACCCCGTGTCACGCTACCGCTTTACCCTCAGCGCCAGGACGCAGGTGGGCTCTGG
  GGAAGCCGTCACAGAGGAGTCACCAGCACCCCCGAATGAAGCTACTCCAACCGCAGCTTACACCAACAAC
  CAAGCGGACATCGCCACCCAGGGCTGGTTCATTGGGCTTATGTGCGCCATCGCCCTCCTGGTGCTGATCC
  TGCTCATCGTCTGTTTCATCAAGAGGAGTCGCGGCGGCAAGTACCCAGTACGAGAAAAGAAGGATGTTCC
  CCTTGGCCCTGAAGACCCCAAGGAAGAGGATGGCTCATTTGACTATAGTGATGAGGACAACAAGCCCCTG
  CAGGGCAGTCAGACATCTCTGGACGGCACCATCAAGCAGCAGGAGAGTGACGACAGCCTGGTGGACTATG
  GCGAGGGTGGCGAGGGTCAGTTCAATGAAGACGGCTCCTTCATCGGCCAGTACACGGTCAAAAAGGACAA
  GGAGGAAACAGAGGGCAACGAAAGCTCAGAGGCCACGTCACCTGTCAATGCTATCTACTCTCTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG228652 representing NM_001160331
  Red=Cloning site Green=Tags(s)
  
  MARQPPPPWVHAAFLLCLLSLGGAIEIPMDLTQPPTITKQSAKDHIVDPRDNILIECEAKGNPAPSFHWT
  RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN
  LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR
  FHFTHTIQQKNPFTLKVLTNHPYNDSSLRNHPDMYSARGVAERTPSFMYPQGTASSQMVLRGMDLLLECI
  ASGVPTPDIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW
  LDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN
  TSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH
  VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDP
  SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNR
  LAALPKGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYPGSVNSAV
  LRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTRKNNMEITWTPMNATSAFGP
  NLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPESVIGYSGEDYPRAAP
  TEVKVRVMNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRLRGVVSRLF
  PYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGIMIGYTLKY
  VAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTEESPAPPNEATPTAAYTNN
  QADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPL
  QGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001160331
• ORF Size: 3567 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001160331.1, NP_001153803.1
• RefSeq Size: 9960 bp
• RefSeq ORF: 3570 bp
• Locus ID: 23114
• UniProt ID: O94856
• Cytogenetics: 1q32.1
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]