GEMC1 (GMNC) (NM_001146686) Human Tagged ORF Clone

CAT#: RC228266

GMNC (Myc-DDK-tagged)-Human geminin coiled-coil domain-containing protein 1 (GEMC1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_001146686" in other vectors (4)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


Rabbit Polyclonal GEMC1 Antibody
    • 100 ug

USD 570.00

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GEMC1
Synonyms GEMC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC228266 representing NM_001146686
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACACCATTCTGCCTTGCCAAGACCAGTACTTTGTAGGAGGCCAGAGCTATAATTGCCCGTATTCCA
CTACAACGTCAGAATCTAGTGTTGACGTTTCCACGGAGACTTGGGTCTCTTTCTGGGCTGCTGGTCTCCT
GGACAACAGAGAGCTCCAACAAGCACCACAGGCACAGGAATCATTCAGTGACTCAAATTTTCCTCTTCCG
GACTTGTGCTCATGGGAAGAGGCTCAGCTTTCCTCTCAGCTCTACAGAAATAAGCAGCTGCAAGATACCC
TGGTGCAGAAGGAAGAAGAACTCGCCAGGTTACACGAAGAGAATAATCACCTCAGACAATACCTGAATTC
TGCTTTGGTTAAATGTCTTGAAGAAAAGGCCAAGAAATTGCTCTCATCTGATGAGTTCTCCAAAGCATAT
GGAAAATTCAGGAAGGGGAAGAGAAAATCCAAAGAGCAAAGATACTCTCCTGCTGAGATTCCCCATCCCA
AAAATGCCAAAAGAAACCTCTCTAGTGAATTTGCTAACTGTGAAGAACAAGCTGGGCCCCCTGTGGATCC
CTGGGTCCTTCAAACACTTGGGTTAAAAGACCTTGACACTATCGATGACACCTCATCAGCTAACTACAGT
GCCCTCGCATCTCATCCCAGAAGAGTCGCCAGCACATTTTCCCAGTTTCCGGATGATGCAGTTGATTATA
AAAATATCCCCAGAGAGGATATGCCAATTGACTATAGAGGTGACAGAACAACCCCCTTGCACAGCACTGC
CACTCATGGAGAAGATTTCCACATCCTTTCTCAACTTTCAAATCCCCCAGTGGGGCTGAAAACTCTTCCT
TACTATACTGCTCATGTGTCACCCAACAAGACAGAGATGGCATTTTCCACATCCCTGAGCCCTCATTGTA
ATGTGAAAACTCATTCCTTCCACCAGGGACAAGCCTTTGTGCGTCGAGATGAGGAGGGAGGCTGGAAGTT
TACCTGGGTCCCTAAGCAGTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC228266 representing NM_001146686
Red=Cloning site Green=Tags(s)

MNTILPCQDQYFVGGQSYNCPYSTTTSESSVDVSTETWVSFWAAGLLDNRELQQAPQAQESFSDSNFPLP
DLCSWEEAQLSSQLYRNKQLQDTLVQKEEELARLHEENNHLRQYLNSALVKCLEEKAKKLLSSDEFSKAY
GKFRKGKRKSKEQRYSPAEIPHPKNAKRNLSSEFANCEEQAGPPVDPWVLQTLGLKDLDTIDDTSSANYS
ALASHPRRVASTFSQFPDDAVDYKNIPREDMPIDYRGDRTTPLHSTATHGEDFHILSQLSNPPVGLKTLP
YYTAHVSPNKTEMAFSTSLSPHCNVKTHSFHQGQAFVRRDEEGGWKFTWVPKQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001146686
ORF Size 1002 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001146686.3
RefSeq ORF 1005 bp
Locus ID 647309
UniProt ID A6NCL1
Cytogenetics 3q28
MW 37.7 kDa
Gene Summary Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.