GEMC1 (GMNC) (NM_001146686) Human Tagged ORF Clone

CAT#: RC228266

GMNC (Myc-DDK-tagged)-Human geminin coiled-coil domain-containing protein 1 (GEMC1)

Product Images

Circular map for RC228266

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  Western blot validation of overexpression lysate (Cat# LY431294) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228266 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GEMC1
• Synonyms: GEMC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC228266 representing NM_001146686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACCATTCTGCCTTGCCAAGACCAGTACTTTGTAGGAGGCCAGAGCTATAATTGCCCGTATTCCA
  CTACAACGTCAGAATCTAGTGTTGACGTTTCCACGGAGACTTGGGTCTCTTTCTGGGCTGCTGGTCTCCT
  GGACAACAGAGAGCTCCAACAAGCACCACAGGCACAGGAATCATTCAGTGACTCAAATTTTCCTCTTCCG
  GACTTGTGCTCATGGGAAGAGGCTCAGCTTTCCTCTCAGCTCTACAGAAATAAGCAGCTGCAAGATACCC
  TGGTGCAGAAGGAAGAAGAACTCGCCAGGTTACACGAAGAGAATAATCACCTCAGACAATACCTGAATTC
  TGCTTTGGTTAAATGTCTTGAAGAAAAGGCCAAGAAATTGCTCTCATCTGATGAGTTCTCCAAAGCATAT
  GGAAAATTCAGGAAGGGGAAGAGAAAATCCAAAGAGCAAAGATACTCTCCTGCTGAGATTCCCCATCCCA
  AAAATGCCAAAAGAAACCTCTCTAGTGAATTTGCTAACTGTGAAGAACAAGCTGGGCCCCCTGTGGATCC
  CTGGGTCCTTCAAACACTTGGGTTAAAAGACCTTGACACTATCGATGACACCTCATCAGCTAACTACAGT
  GCCCTCGCATCTCATCCCAGAAGAGTCGCCAGCACATTTTCCCAGTTTCCGGATGATGCAGTTGATTATA
  AAAATATCCCCAGAGAGGATATGCCAATTGACTATAGAGGTGACAGAACAACCCCCTTGCACAGCACTGC
  CACTCATGGAGAAGATTTCCACATCCTTTCTCAACTTTCAAATCCCCCAGTGGGGCTGAAAACTCTTCCT
  TACTATACTGCTCATGTGTCACCCAACAAGACAGAGATGGCATTTTCCACATCCCTGAGCCCTCATTGTA
  ATGTGAAAACTCATTCCTTCCACCAGGGACAAGCCTTTGTGCGTCGAGATGAGGAGGGAGGCTGGAAGTT
  TACCTGGGTCCCTAAGCAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC228266 representing NM_001146686
  Red=Cloning site Green=Tags(s)
  
  MNTILPCQDQYFVGGQSYNCPYSTTTSESSVDVSTETWVSFWAAGLLDNRELQQAPQAQESFSDSNFPLP
  DLCSWEEAQLSSQLYRNKQLQDTLVQKEEELARLHEENNHLRQYLNSALVKCLEEKAKKLLSSDEFSKAY
  GKFRKGKRKSKEQRYSPAEIPHPKNAKRNLSSEFANCEEQAGPPVDPWVLQTLGLKDLDTIDDTSSANYS
  ALASHPRRVASTFSQFPDDAVDYKNIPREDMPIDYRGDRTTPLHSTATHGEDFHILSQLSNPPVGLKTLP
  YYTAHVSPNKTEMAFSTSLSPHCNVKTHSFHQGQAFVRRDEEGGWKFTWVPKQS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146686
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146686.3
• RefSeq ORF: 1005 bp
• Locus ID: 647309
• UniProt ID: A6NCL1
• Cytogenetics: 3q28
• MW: 37.7 kDa
• Gene Summary: Regulator of DNA replication. Promotes initiation of chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent recruitment of CDC45L onto replication origins (By similarity).[UniProtKB/Swiss-Prot Function]