STIM2 (NM_020860) Human Tagged ORF Clone

CAT#: RC224102

Reviews ()
Write a review

STIM2 (Myc-DDK-tagged)-Human stromal interaction molecule 2 (STIM2), transcript variant 2



  "NM_020860" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 930.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol STIM2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224102 representing NM_020860
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGCAGCCGGGATCAGAGCTCCGGAGGCCGCCGGTGCCGATGGGACCAGGCTGGCGCCCGGCGGGA
GCCCGTGTCTGAGGCGGCGGGGGCGGCCGGAGGAGTCGCCGGCGGCGGTGGTGGCGCCTCGCGGAGCCGG
CGAGCTGCAGGCGGCCGGGGCGCCGCTGCGCTTTCACCCGGCTTCTCCTCGGCGCCTTCATCCCGCCTCG
ACTCCTGGCCCAGCGTGGGGCTGGCTGCTGCGGCGGCGGCGCTGGGCTGCGTTGCTGGTGCTCGGGCTGC
TGGTAGCCGGAGCGGCGGACGGATGCGAGCTTGTGCCCCGGCACCTCCGCGGGCGGCGGGCGACTGGCTC
TGCCGCAACTGCCGCCTCCTCTCCCGCCGCGGCGGCCGGCGATAGCCCGGCGCTCATGACAGATCCCTGC
ATGTCACTGAGTCCACCATGCTTTACAGAAGAAGACAGATTTAGTCTGGAAGCTCTTCAAACAATACATA
AACAAATGGATGATGACAAAGATGGTGGAATTGAAGTAGAGGAAAGTGATGAATTCATCAGAGAAGATAT
GAAATATAAAGATGCTACTAATAAACACAGCCATCTGCACAGAGAAGATAAACATATAACGATTGAGGAT
TTATGGAAACGATGGAAAACATCAGAAGTTCATAATTGGACCCTTGAAGACACTCTTCAGTGGTTGATAG
AGTTTGTTGAACTACCCCAATATGAGAAGAATTTTAGAGACAACAATGTCAAAGGAACGACACTTCCCAG
GATAGCAGTGCACGAACCTTCATTTATGATCTCCCAGTTGAAAATCAGTGACCGGAGTCACAGACAAAAA
CTTCAGCTCAAGGCATTGGATGTGGTTTTGTTTGGACCTCTAACACGCCCACCTCATAACTGGATGAAAG
ATTTTATCCTCACAGTTTCTATAGTAATTGGTGTTGGAGGCTGCTGGTTTGCTTATACGCAGAATAAGAC
ATCAAAAGAACATGTTGCAAAAATGATGAAAGATTTAGAGAGCTTACAAACTGCAGAGCAAAGTCTAATG
GACTTACAAGAGAGGCTTGAAAAGGCACAGGAAGAAAACAGAAATGTTGCTGTAGAAAAGCAAAATTTAG
AGCGCAAAATGATGGATGAAATCAATTATGCAAAGGAGGAGGCTTGTCGGCTGAGAGAGCTAAGGGAGGG
AGCTGAATGTGAATTGAGTAGACGTCAGTATGCAGAACAGGAATTGGAACAGGTTCGCATGGCTCTGAAA
AAGGCCGAAAAAGAATTTGAACTGAGAAGCAGTTGGTCTGTTCCAGATGCACTTCAGAAATGGCTTCAGT
TAACACATGAAGTAGAAGTGCAATACTACAATATTAAAAGACAAAACGCTGAAATGCAGCTAGCTATTGC
TAAAGATGAGGCAGAAAAAATTAAAAAGAAGAGAAGCACAGTCTTTGGGACTCTGCACGTTGCACACAGC
TCCTCCCTAGATGAGGTAGACCACAAAATTCTGGAAGCAAAGAAAGCTCTCTCTGAGTTGACAACTTGTT
TACGAGAACGACTTTTTCGCTGGCAACAAATTGAGAAGATCTGTGGCTTTCAGATAGCCCATAACTCAGG
ACTCCCCAGCCTGACCTCTTCCCTTTATTCTGATCACAGCTGGGTGGTGATGCCCAGAGTCTCCATTCCA
CCCTATCCAATTGCTGGAGGAGTTGATGACTTAGATGAAGACACACCCCCAATAGTGTCACAATTTCCCG
GGACCATGGCTAAACCTCCTGGATCATTAGCCAGAAGCAGCAGCCTGTGCCGTTCACGCCGCAGCATTGT
GCCGTCCTCGCCTCAGCCTCAGCGAGCTCAGCTTGCTCCACACGCCCCCCACCCGTCACACCCTCGGCAC
CCTCACCACCCGCAACACACACCACACTCCTTGCCTTCCCCTGATCCAGATATCCTCTCAGTGTCAAGTT
GCCCTGCGCTTTATCGAAATGAAGAGGAGGAAGAGGCCATTTACTTCTCTGCTGAAAAGCAATGGGAAGT
GCCAGACACAGCTTCAGAATGTGACTCCTTAAATTCTTCCATTGGAAGGAAACAGTCTCCTCCTTTAAGC
CTCGAGATATACCAAACATTATCTCCGCGAAAGATATCAAGAGATGAGGTGTCCCTAGAGGATTCCTCCC
GAGGGGATTCGCCTGTAACTGTGGATGTGTCTTGGGGTTCTCCCGACTGTGTAGGTCTGACAGAAACTAA
GAGTATGATCTTCAGTCCTGCAAGCAAAGTGTACAATGGCATTTTGGAGAAATCCTGTAGCATGAACCAG
CTTTCCAGTGGCATCCCGGTGCCTAAACCTCGCCACACATCATGTTCCTCAGCTGGCAACGACAGTAAAC
CAGTTCAGGAAGCCCCAAGTGTTGCCAGAATAAGCAGCATCCCACATGACCTTTGTCATAATGGAGAGAA
AAGCAAAAAGCCATCAAAAATCAAAAGCCTTTTTAAGAAGAAATCTAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224102 representing NM_020860
Red=Cloning site Green=Tags(s)

MNAAGIRAPEAAGADGTRLAPGGSPCLRRRGRPEESPAAVVAPRGAGELQAAGAPLRFHPASPRRLHPAS
TPGPAWGWLLRRRRWAALLVLGLLVAGAADGCELVPRHLRGRRATGSAATAASSPAAAAGDSPALMTDPC
MSLSPPCFTEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIED
LWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQK
LQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLM
DLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALK
KAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHS
SSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIP
PYPIAGGVDDLDEDTPPIVSQFPGTMAKPPGSLARSSSLCRSRRSIVPSSPQPQRAQLAPHAPHPSHPRH
PHHPQHTPHSLPSPDPDILSVSSCPALYRNEEEEEAIYFSAEKQWEVPDTASECDSLNSSIGRKQSPPLS
LEIYQTLSPRKISRDEVSLEDSSRGDSPVTVDVSWGSPDCVGLTETKSMIFSPASKVYNGILEKSCSMNQ
LSSGIPVPKPRHTSCSSAGNDSKPVQEAPSVARISSIPHDLCHNGEKSKKPSKIKSLFKKKSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020860
ORF Size 2499 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020860.2
RefSeq Size 5156 bp
RefSeq ORF 2241 bp
Locus ID 57620
UniProt ID Q9P246
Cytogenetics 4p15.2
MW 82.6 kDa
Gene Summary This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]

Other Versions

Other products for "STIM2"
Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


Rabbit Polyclonal STIM2 Antibody
    • 100 ug

USD 484.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.