STIM2 (NM_020860) Human Tagged ORF Clone

CAT#: RG224102

STIM2 (tGFP-tagged) - Human stromal interaction molecule 2 (STIM2), transcript variant 2

Product Images

Circular map for RG224102

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: STIM2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224102 representing NM_020860
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCAGCCGGGATCAGAGCTCCGGAGGCCGCCGGTGCCGATGGGACCAGGCTGGCGCCCGGCGGGA
  GCCCGTGTCTGAGGCGGCGGGGGCGGCCGGAGGAGTCGCCGGCGGCGGTGGTGGCGCCTCGCGGAGCCGG
  CGAGCTGCAGGCGGCCGGGGCGCCGCTGCGCTTTCACCCGGCTTCTCCTCGGCGCCTTCATCCCGCCTCG
  ACTCCTGGCCCAGCGTGGGGCTGGCTGCTGCGGCGGCGGCGCTGGGCTGCGTTGCTGGTGCTCGGGCTGC
  TGGTAGCCGGAGCGGCGGACGGATGCGAGCTTGTGCCCCGGCACCTCCGCGGGCGGCGGGCGACTGGCTC
  TGCCGCAACTGCCGCCTCCTCTCCCGCCGCGGCGGCCGGCGATAGCCCGGCGCTCATGACAGATCCCTGC
  ATGTCACTGAGTCCACCATGCTTTACAGAAGAAGACAGATTTAGTCTGGAAGCTCTTCAAACAATACATA
  AACAAATGGATGATGACAAAGATGGTGGAATTGAAGTAGAGGAAAGTGATGAATTCATCAGAGAAGATAT
  GAAATATAAAGATGCTACTAATAAACACAGCCATCTGCACAGAGAAGATAAACATATAACGATTGAGGAT
  TTATGGAAACGATGGAAAACATCAGAAGTTCATAATTGGACCCTTGAAGACACTCTTCAGTGGTTGATAG
  AGTTTGTTGAACTACCCCAATATGAGAAGAATTTTAGAGACAACAATGTCAAAGGAACGACACTTCCCAG
  GATAGCAGTGCACGAACCTTCATTTATGATCTCCCAGTTGAAAATCAGTGACCGGAGTCACAGACAAAAA
  CTTCAGCTCAAGGCATTGGATGTGGTTTTGTTTGGACCTCTAACACGCCCACCTCATAACTGGATGAAAG
  ATTTTATCCTCACAGTTTCTATAGTAATTGGTGTTGGAGGCTGCTGGTTTGCTTATACGCAGAATAAGAC
  ATCAAAAGAACATGTTGCAAAAATGATGAAAGATTTAGAGAGCTTACAAACTGCAGAGCAAAGTCTAATG
  GACTTACAAGAGAGGCTTGAAAAGGCACAGGAAGAAAACAGAAATGTTGCTGTAGAAAAGCAAAATTTAG
  AGCGCAAAATGATGGATGAAATCAATTATGCAAAGGAGGAGGCTTGTCGGCTGAGAGAGCTAAGGGAGGG
  AGCTGAATGTGAATTGAGTAGACGTCAGTATGCAGAACAGGAATTGGAACAGGTTCGCATGGCTCTGAAA
  AAGGCCGAAAAAGAATTTGAACTGAGAAGCAGTTGGTCTGTTCCAGATGCACTTCAGAAATGGCTTCAGT
  TAACACATGAAGTAGAAGTGCAATACTACAATATTAAAAGACAAAACGCTGAAATGCAGCTAGCTATTGC
  TAAAGATGAGGCAGAAAAAATTAAAAAGAAGAGAAGCACAGTCTTTGGGACTCTGCACGTTGCACACAGC
  TCCTCCCTAGATGAGGTAGACCACAAAATTCTGGAAGCAAAGAAAGCTCTCTCTGAGTTGACAACTTGTT
  TACGAGAACGACTTTTTCGCTGGCAACAAATTGAGAAGATCTGTGGCTTTCAGATAGCCCATAACTCAGG
  ACTCCCCAGCCTGACCTCTTCCCTTTATTCTGATCACAGCTGGGTGGTGATGCCCAGAGTCTCCATTCCA
  CCCTATCCAATTGCTGGAGGAGTTGATGACTTAGATGAAGACACACCCCCAATAGTGTCACAATTTCCCG
  GGACCATGGCTAAACCTCCTGGATCATTAGCCAGAAGCAGCAGCCTGTGCCGTTCACGCCGCAGCATTGT
  GCCGTCCTCGCCTCAGCCTCAGCGAGCTCAGCTTGCTCCACACGCCCCCCACCCGTCACACCCTCGGCAC
  CCTCACCACCCGCAACACACACCACACTCCTTGCCTTCCCCTGATCCAGATATCCTCTCAGTGTCAAGTT
  GCCCTGCGCTTTATCGAAATGAAGAGGAGGAAGAGGCCATTTACTTCTCTGCTGAAAAGCAATGGGAAGT
  GCCAGACACAGCTTCAGAATGTGACTCCTTAAATTCTTCCATTGGAAGGAAACAGTCTCCTCCTTTAAGC
  CTCGAGATATACCAAACATTATCTCCGCGAAAGATATCAAGAGATGAGGTGTCCCTAGAGGATTCCTCCC
  GAGGGGATTCGCCTGTAACTGTGGATGTGTCTTGGGGTTCTCCCGACTGTGTAGGTCTGACAGAAACTAA
  GAGTATGATCTTCAGTCCTGCAAGCAAAGTGTACAATGGCATTTTGGAGAAATCCTGTAGCATGAACCAG
  CTTTCCAGTGGCATCCCGGTGCCTAAACCTCGCCACACATCATGTTCCTCAGCTGGCAACGACAGTAAAC
  CAGTTCAGGAAGCCCCAAGTGTTGCCAGAATAAGCAGCATCCCACATGACCTTTGTCATAATGGAGAGAA
  AAGCAAAAAGCCATCAAAAATCAAAAGCCTTTTTAAGAAGAAATCTAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224102 representing NM_020860
  Red=Cloning site Green=Tags(s)
  
  MNAAGIRAPEAAGADGTRLAPGGSPCLRRRGRPEESPAAVVAPRGAGELQAAGAPLRFHPASPRRLHPAS
  TPGPAWGWLLRRRRWAALLVLGLLVAGAADGCELVPRHLRGRRATGSAATAASSPAAAAGDSPALMTDPC
  MSLSPPCFTEEDRFSLEALQTIHKQMDDDKDGGIEVEESDEFIREDMKYKDATNKHSHLHREDKHITIED
  LWKRWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHEPSFMISQLKISDRSHRQK
  LQLKALDVVLFGPLTRPPHNWMKDFILTVSIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLM
  DLQERLEKAQEENRNVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALK
  KAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHS
  SSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIP
  PYPIAGGVDDLDEDTPPIVSQFPGTMAKPPGSLARSSSLCRSRRSIVPSSPQPQRAQLAPHAPHPSHPRH
  PHHPQHTPHSLPSPDPDILSVSSCPALYRNEEEEEAIYFSAEKQWEVPDTASECDSLNSSIGRKQSPPLS
  LEIYQTLSPRKISRDEVSLEDSSRGDSPVTVDVSWGSPDCVGLTETKSMIFSPASKVYNGILEKSCSMNQ
  LSSGIPVPKPRHTSCSSAGNDSKPVQEAPSVARISSIPHDLCHNGEKSKKPSKIKSLFKKKSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020860
• ORF Size: 2499 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020860.2
• RefSeq Size: 5156 bp
• RefSeq ORF: 2241 bp
• Locus ID: 57620
• UniProt ID: Q9P246
• Cytogenetics: 4p15.2
• Gene Summary: This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]