TRIM32 (NM_001099679) Human Tagged ORF Clone

CAT#: RC223796

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TRIM32 (Myc-DDK-tagged)-Human tripartite motif containing 32 (TRIM32), transcript variant 2



Reconstitution Protocol

USD 911.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM32
Synonyms BBS11; HT2A; LGMD2H; LGMDR8; TATIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC223796 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCAGCAGCAGCTTCTCACCTGAACCTGGATGCCCTCCGGGAAGTGCTAGAATGCCCCATCTGCA
TGGAGTCCTTCACAGAAGAGCAGCTGCGTCCCAAGCTTCTGCACTGTGGCCATACCATCTGCCGCCAGTG
CCTGGAGAAGCTATTGGCCAGTAGCATCAATGGTGTCCGCTGTCCCTTTTGCAGCAAGATTACCCGCATA
ACCAGCTTGACCCAGCTGACAGACAATCTGACAGTGCTAAAGATCATTGATACAGCTGGGCTCAGCGAGG
CTGTGGGGCTGCTCATGTGTCGGTCCTGTGGGCGGCGTCTGCCCCGGCAATTCTGCCGGAGCTGTGGTTT
GGTGTTATGTGAGCCCTGCCGGGAGGCAGACCATCAGCCTCCTGGCCACTGTACACTCCCTGTCAAAGAA
GCAGCTGAGGAGCGGCGTCGGGACTTTGGAGAGAAGTTAACTCGTCTGCGGGAACTTATGGGGGAGCTGC
AGCGGCGGAAGGCAGCCTTGGAAGGTGTCTCCAAGGACCTTCAGGCAAGGTATAAAGCAGTTCTCCAGGA
GTATGGGCATGAGGAGCGCAGGGTCCAGGATGAGCTGGCTCGCTCTCGGAAGTTCTTCACAGGCTCTTTG
GCTGAAGTTGAGAAGTCCAATAGTCAAGTGGTAGAGGAGCAGAGTTACCTGCTTAACATTGCAGAGGTGC
AGGCTGTGTCTCGCTGTGACTACTTCCTGGCCAAGATCAAGCAGGCAGATGTAGCACTACTGGAGGAGAC
AGCTGATGAGGAGGAGCCAGAGCTCACTGCCAGCTTGCCTCGGGAGCTCACCCTGCAAGATGTGGAGCTC
CTTAAGGTAGGTCATGTTGGCCCCCTCCAAATTGGACAAGCTGTTAAGAAGCCCCGGACAGTTAACGTGG
AAGATTCCTGGGCCATGGAGGCCACAGCGTCTGCTGCCTCTACCTCTGTTACTTTTAGAGAGATGGACAT
GAGCCCGGAGGAAGTGGTTGCCAGCCCTAGGGCCTCACCTGCTAAACAGCGGGGTCCTGAGGCAGCCTCC
AATATCCAGCAGTGCCTCTTTCTCAAGAAGATGGGGGCCAAAGGCAGCACTCCAGGAATGTTCAATCTTC
CAGTCAGTCTCTACGTGACCAGTCAAGGTGAAGTACTAGTCGCTGACCGTGGTAACTATCGTATACAAGT
CTTTACCCGCAAAGGCTTTTTGAAGGAAATCCGCCGCAGCCCCAGTGGCATTGATAGCTTTGTGCTAAGC
TTCCTTGGGGCAGATCTACCCAACCTCACTCCTCTCTCAGTGGCAATGAACTGCCAGGGGCTGATTGGTG
TGACTGACAGCTATGATAACTCCCTCAAGGTATATACCTTGGATGGCCACTGCGTGGCCTGTCACAGGAG
CCAGCTGAGCAAACCATGGGGTATCACAGCCTTGCCATCTGGCCAGTTTGTAGTAACCGATGTGGAAGGT
GGAAAGCTTTGGTGTTTCACAGTTGATCGAGGATCAGGGGTGGTCAAATACAGCTGCCTATGTAGTGCTG
TGCGGCCCAAATTTGTCACCTGTGATGCTGAGGGCACCGTCTACTTCACCCAGGGCTTAGGCCTCAATCT
GGAGAATCGGCAGAATGAGCACCACCTGGAGGGTGGCTTTTCCATTGGCTCTGTAGGCCCTGATGGGCAG
CTGGGTCGCCAGATTAGCCACTTCTTCTCGGAGAATGAGGATTTCCGCTGCATTGCTGGCATGTGTGTGG
ATGCTCGTGGTGATCTCATCGTGGCTGACAGTAGTCGCAAGGAAATTCTCCATTTTCCTAAGGGTGGGGG
CTATAGTGTCCTTATTCGAGAGGGACTTACCTGTCCGGTGGGCATAGCCCTAACTCCTAAGGGGCAGCTG
CTGGTCTTGGACTGTTGGGATCATTGCATCAAGATCTACAGCTACCATCTGAGAAGATATTCCACCCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC223796 protein sequence
Red=Cloning site Green=Tags(s)

MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRI
TSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKE
AAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSL
AEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVEL
LKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAAS
NIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLS
FLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEG
GKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQ
LGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQL
LVLDCWDHCIKIYSYHLRRYSTP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001099679
ORF Size 1959 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001099679.2
RefSeq Size 3731 bp
RefSeq ORF 1962 bp
Locus ID 22954
UniProt ID Q13049
Cytogenetics 9q33.1
Protein Families Transcription Factors
Protein Pathways Ubiquitin mediated proteolysis
MW 72 kDa
Gene Summary The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. [provided by RefSeq, Jul 2008]
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