NOS1 (NM_000620) Human Tagged Lenti ORF Clone

SKU
RC222384L4
Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, mGFP tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$1,527.00
In Stock*
Specifications
Product Data
Target Symbol NOS1
Synonyms bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC222384).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_000620
ORF Size 4302 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000620.1
RefSeq Size 7124 bp
RefSeq ORF 4305 bp
Locus ID 4842
UniProt ID P29475
Cytogenetics 12q24.22
Protein Families Druggable Genome
Protein Pathways Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer
MW 160.8 kDa
Summary The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]
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SKU Description Size Price
RC222384 NOS1 (Myc-DDK-tagged)-Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 10 ug
$1,227.00
RC222384L1 Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,527.00
RC222384L2 Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, mGFP tagged 10 ug
$1,527.00
RC222384L3 Lenti ORF clone of Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,527.00
RG222384 NOS1 (tGFP-tagged) - Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 10 ug
$789.00 MSRP $1,427.00 MSRP $1,427.00
SC300110 NOS1 (untagged)-Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1 10 ug
$1,227.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.