GRIK2 (NM_021956) Human Tagged ORF Clone

CAT#: RC222369

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GRIK2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1



  "NM_021956" in other vectors (6)

Reconstitution Protocol

USD 831.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GRIK2
Synonyms EAA4; GLR6; GluK2; GLUK6; GLUR6; MRT6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222369 representing NM_021956
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATTATTTTCCCGATTCTAAGTAATCCAGTCTTCAGGCGCACCGTTAAACTCCTGCTCTGTTTAC
TGTGGATTGGATATTCTCAAGGAACCACACATGTATTAAGATTTGGTGGTATTTTTGAATATGTGGAATC
TGGCCCAATGGGAGCTGAGGAACTTGCATTCAGATTTGCTGTGAACACAATTAACAGAAACAGAACATTG
CTACCCAATACTACCCTTACCTATGATACCCAGAAGATAAACCTTTATGATAGTTTTGAAGCATCCAAGA
AAGCCTGTGATCAGCTGTCTCTTGGGGTGGCTGCCATCTTCGGGCCTTCACACAGCTCATCAGCAAACGC
AGTGCAGTCCATCTGCAATGCTCTGGGAGTTCCCCACATACAGACCCGCTGGAAGCACCAGGTGTCAGAC
AACAAAGATTCCTTCTATGTCAGTCTCTACCCAGACTTCTCTTCACTCAGCCGTGCCATTTTAGACCTGG
TGCAGTTTTTCAAGTGGAAAACCGTCACGGTTGTGTATGATGACAGCACTGGTCTCATTCGTTTGCAAGA
GCTCATCAAAGCTCCATCAAGGTATAATCTTCGACTCAAAATTCGTCAGTTACCTGCTGATACAAAGGAT
GCAAAACCCTTACTAAAAGAAATGAAAAGAGGCAAGGAGTTTCATGTAATCTTTGATTGTAGCCATGAAA
TGGCAGCAGGCATTTTAAAACAGGCATTAGCTATGGGAATGATGACAGAATACTATCATTATATCTTTAC
CACTCTGGACCTCTTTGCTCTTGATGTTGAGCCCTACCGATACAGTGGTGTTAACATGACAGGGTTCAGA
ATATTAAATACAGAAAATACCCAAGTCTCCTCCATCATTGAAAAGTGGTCGATGGAACGATTGCAGGCAC
CTCCGAAACCCGATTCAGGTTTGCTGGATGGATTTATGACGACTGATGCTGCTCTAATGTATGATGCTGT
GCATGTGGTGTCTGTGGCCGTTCAACAGTTTCCCCAGATGACAGTCAGTTCCTTGCAGTGTAATCGACAT
AAACCCTGGCGCTTCGGGACCCGCTTTATGAGTCTAATTAAAGAGGCACATTGGGAAGGCCTCACAGGCA
GAATAACTTTCAACAAAACCAATGGCTTGAGAACAGATTTTGATTTGGATGTGATCAGTCTGAAGGAAGA
AGGTCTAGAAAAGATTGGAACGTGGGATCCAGCCAGTGGCCTGAATATGACAGAAAGTCAAAAGGGAAAG
CCAGCGAACATCACAGATTCCTTATCCAATCGTTCTTTGATTGTTACCACCATTTTGGAAGAGCCTTATG
TCCTTTTTAAGAAGTCTGACAAACCTCTCTATGGTAATGATCGATTTGAAGGCTATTGCATTGATCTCCT
CAGAGAGTTATCTACAATCCTTGGCTTTACATATGAAATTAGACTTGTGGAAGATGGGAAATATGGAGCC
CAGGATGATGCCAATGGACAATGGAATGGAATGGTTCGTGAACTAATTGATCATAAAGCTGACCTTGCAG
TTGCTCCACTGGCTATTACCTATGTTCGAGAGAAGGTCATCGACTTTTCCAAGCCCTTTATGACACTTGG
AATAAGTATTTTGTACCGCAAGCCCAATGGTACAAACCCAGGCGTCTTCTCCTTCCTGAATCCTCTCTCC
CCTGATATCTGGATGTATGTTCTGCTGGCTTACTTGGGTGTCAGTTGTGTGCTCTTTGTCATAGCCAGGT
TTAGTCCTTATGAGTGGTATAATCCACACCCTTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTAC
CTTGCTAAATAGTTTCTGGTTTGGGGTTGGAGCTCTCATGCGGCAAGGTTCTGAGCTCATGCCCAAAGCA
CTGTCCACCAGGATAGTGGGAGGCATTTGGTGGTTTTTCACACTTATCATCATTTCTTCGTATACTGCTA
ACTTAGCCGCCTTTCTGACAGTGGAACGCATGGAATCCCCTATTGACTCTGCTGATGATTTAGCTAAACA
AACCAAGATAGAATATGGAGCAGTAGAGGATGGTGCAACCATGACTTTTTTCAAGAAATCAAAAATCTCC
ACGTATGACAAAATGTGGGCCTTTATGAGTAGCAGAAGGCAGTCAGTGCTGGTCAAAAGTAATGAAGAAG
GAATCCAGCGAGTCCTCACCTCTGATTATGCTTTCCTAATGGAGTCAACAACCATCGAGTTTGTTACCCA
GCGGAACTGTAACCTGACACAGATTGGCGGCCTTATAGACTCTAAAGGTTATGGCGTTGGCACTCCCATG
GGTTCTCCATATCGAGACAAAATTACCATAGCAATTCTTCAGCTGCAAGAGGAAGGCAAACTGCATATGA
TGAAGGAGAAATGGTGGAGGGGCAATGGTTGCCCAGAAGAGGAAAGCAAAGAGGCCAGTGCCCTGGGGGT
TCAGAATATTGGTGGCATCTTCATTGTTCTGGCAGCCGGCTTGGTGCTTTCAGTTTTTGTGGCAGTGGGA
GAATTTTTATACAAATCCAAAAAAAACGCTCAATTGGAAAAGAGGTCCTTCTGTAGTGCCATGGTAGAAG
AATTGAGGATGTCCCTGAAGTGCCAGCGTCGGTTAAAACATAAGCCACAGGCCCCAGTTATTGTGAAAAC
AGAAGAAGTTATCAACATGCACACATTTAACGACAGAAGGTTGCCAGGTAAAGAAACCATGGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222369 representing NM_021956
Red=Cloning site Green=Tags(s)

MKIIFPILSNPVFRRTVKLLLCLLWIGYSQGTTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTL
LPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSD
NKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKD
AKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFR
ILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRH
KPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGK
PANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGA
QDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLS
PDIWMYVLLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMRQGSELMPKA
LSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKIS
TYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPM
GSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVG
EFLYKSKKNAQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_021956
ORF Size 2724 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021956.5
RefSeq Size 3322 bp
RefSeq ORF 2727 bp
Locus ID 2898
UniProt ID Q13002
Cytogenetics 6q16.3
Domains lig_chan, ANF_receptor
Protein Families Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 98.9 kDa
Gene Summary Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability. [provided by RefSeq, Jul 2008]

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