ZNF367 (NM_153695) Human Tagged ORF Clone

CAT#: RC221909

ZNF367 (Myc-DDK-tagged)-Human zinc finger protein 367 (ZNF367)

Product images

Western blot validation of overexpression lysate (Cat# LY406979) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221909 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: ZNF367 (NM_153695) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF367
• Synonyms: AFF29; CDC14B; ZFF29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221909 representing NM_153695
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCGGGGCTTCGAGGCGCCCATGGCGGAGAACCCGCCGCCGCCGCCGCCGCCCGTCATCTTCTGCC
  ACGACTCCCCGAAGCGGGTGCTGGTGTCGGTCATCAGGACGACCCCGATCAAGCCAACGTGCGGCGGTGG
  AGGGGAGCCGGAGCCGCCGCCGCCGCTCATCCCCACCAGCCCCGGCTTCAGCGACTTCATGGTGTACCCG
  TGGCGCTGGGGCGAGAACGCACACAACGTGACGCTCAGCCCTGGGGCCGCGGGGGCCGCCGCCTCGGCCG
  CCCTGCCTGCAGCCGCAGCCGCCGAGCACTCGGGGCTTCGTGGCCGGGGCGCGCCCCCGCCCGCCGCCTC
  GGCCTCCGCCGCCGCCTCGGGAGGTGAGGACGAGGAGGAAGCGAGCAGCCCAGACAGCGGCCACCTCAAG
  GATGGAATCCGACGTGGTAGGCCCAGAGCAGATACTGTCCGCGATTTAATAAATGAAGGAGAGCATTCAT
  CCAGCAGAATCCGTTGTAACATCTGTAATAGGGTGTTTCCACGGGAGAAATCGCTCCAGGCTCACAAAAG
  GACTCATACAGGTGAGAGGCCCTATCTGTGTGACTATCCAGACTGTGGAAAAGCCTTTGTTCAAAGTGGA
  CAGCTCAAAACACATCAGCGTCTTCACACCGGAGAGAAACCTTTTGTTTGTTCAGAAAATGGCTGCCTGA
  GCAGATTCACCCATGCAAACCGCCACTGTCCGAAGCACCCCTACGCCAGGCTGAAGAGAGAGGAGCCCAC
  GGACACACTCAGCAAACATCAGGCTGCCGACAACAAGGCCGCGGCCGAGTGGCTGGCGAGGTATTGGGAA
  ATGAGAGAGCAGCGCACCCCCACTTTGAAAGGCAAGCTGGTTCAGAAGGCTGATCAGGAGCAGCAGGACC
  CTCTGGAATACCTTCAGTCTGATGAAGAGGACGACGAGAAGAGAGGGGCCCAGCGCCGGCTGCAGGAGCA
  GCGGGAGCGCCTGCATGGAGCCCTCGCGCTCATAGAGCTTGCCAACCTGACTGGGGCGCCACTCCGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221909 representing NM_153695
  Red=Cloning site Green=Tags(s)
  
  MIRGFEAPMAENPPPPPPPVIFCHDSPKRVLVSVIRTTPIKPTCGGGGEPEPPPPLIPTSPGFSDFMVYP
  WRWGENAHNVTLSPGAAGAAASAALPAAAAAEHSGLRGRGAPPPAASASAAASGGEDEEEASSPDSGHLK
  DGIRRGRPRADTVRDLINEGEHSSSRIRCNICNRVFPREKSLQAHKRTHTGERPYLCDYPDCGKAFVQSG
  QLKTHQRLHTGEKPFVCSENGCLSRFTHANRHCPKHPYARLKREEPTDTLSKHQAADNKAAAEWLARYWE
  MREQRTPTLKGKLVQKADQEQQDPLEYLQSDEEDDEKRGAQRRLQEQRERLHGALALIELANLTGAPLRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153695
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153695.4
• RefSeq Size: 3714 bp
• RefSeq ORF: 1053 bp
• Locus ID: 195828
• Cytogenetics: 9q22.32
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 38.9 kDa
• Gene Summary: Transcriptional activator. Isoform 1 may be involved in transcriptional activation of erythroid genes.[UniProtKB/Swiss-Prot Function]