KIAA0859 (METTL13) (NM_015935) Human Tagged ORF Clone

CAT#: RC221362

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METTL13 (Myc-DDK-tagged)-Human methyltransferase like 13 (METTL13), transcript variant 1



  "NM_015935" in other vectors (4)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol METTL13
Synonyms 5630401D24Rik; CGI-01; DFNB26; DFNB26M; DFNM1; feat; KIAA0859; METTL13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221362 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCTCTTACCTAAAAGTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAGC
GAGGAAAGAAAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACATAAATATATCAA
GCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGTGAGCAACTGTATGATGTGGGC
TATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTCATCAAGCAAATGAAGGAATGTAATGCCACCC
GACGGCCCCAGATGAGCTTCTTGAAGATGGACATGACGCAGATGGAGTTTCCTGATGCCTCGTTCCAGGT
GGTGTTGGACAAGGGCACCCTGGATGCTGTCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGAC
AGGATGCTGGCTGAGGTTGGCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCAGG
CTCACATCCTGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATGGTGAGGGTGCACCAAGTGGC
CAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTCTCCTTGCCTGTCTTTGCCTTCATCATGACC
AAGTTCAGGCCAGTCCCTGGCTCTGCCCTTCAGATCTTTGAGCTGTGTGCTCAGGAGCAGCGCAAGCCTG
TGCGGCTGGAGAGTGCCGAGCGGCTGGCCGAGGCGGTGCAGGAGCGACAGCAGTATGCCTGGCTGTGCAG
CCAGCTGCGCCGCAAGGCCAGGCTGGGGAGTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCA
CGCTACACCCTCCACGTGGTGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTTGCGATTTTCA
TCATCCCTCAGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGCCGGAAACAGCTGGCGGCCAG
TGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGAGGTCAGCAGTATGAAAGCATGGACCACATC
CAAGCTGAGCTGTCGGCTAGAGTCATGGAGCTGGCCCCAGCTGGGATGCCCACCCAGCAGCAGGTCCCCT
TTCTGTCTGTGGGTGGGGACATTGGGGTCCGGACCGTTCAGCACCAAGACTGCAGCCCCTTGAGCGGTGA
CTATGTCATTGAGGATGTGCAAGGGGATGACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGG
AATGTGGTGCAGTCCGAAGCCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAGCGGAAAAAGG
ACAGGAAGAAGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCGGGGCAGTCCATTGATAAGAG
TTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCTGCTGAGAAACCCAGAGCTACTC
CTAGAGATCCCACTGGCATTGTTGGTGGTAGGCCTGGGCGGGGGCAGCCTCCCCCTCTTTGTCCACGATC
ATTTTCCAAAGTCCTGCATTGATGCTGTGGAGATCGATCCCTCCATGTTGGAAGTGGCCACCCAGTGGTT
TGGCTTCTCCCAGAGTGACCGAATGAAGGTCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCA
GGAGGAGGAGAAGCACGGCCTTGCTACGATGTCATAATGTTTGATGTTGACAGTAAGGACCCAACACTGG
GAATGAGTTGTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAGGTTAAAAGCATCTTGACTCC
TGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTGGGGCTAAAAGACTCAGTGCTGGCTGGGCTC
AAGGCAGTGTTCCCCCTCCTATATGTCCGGCGAATTGAGGGTGAAGTGAATGAGATCCTGTTCTGTCAGC
TGCACCCTGAGCAAAAACTTGCCACACCAGAGCTCCTAGAAACAGCCCAGGCTTTGGAGCGGACCCTGAG
GAAGCCTGGGAGGGGTTGGGATGACACGTATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221362 protein sequence
Red=Cloning site Green=Tags(s)

MNLLPKSSREFGSVDYWEKFFQQRGKKAFEWYGTYLELCGVLHKYIKPREKVLVIGCGNSELSEQLYDVG
YRDIVNIDISEVVIKQMKECNATRRPQMSFLKMDMTQMEFPDASFQVVLDKGTLDAVLTDEEEKTLQQVD
RMLAEVGRVLQVGGRYLCISLAQAHILKKAVGHFSREGWMVRVHQVANSQDQVLEAEPQFSLPVFAFIMT
KFRPVPGSALQIFELCAQEQRKPVRLESAERLAEAVQERQQYAWLCSQLRRKARLGSVSLDLCDGDTGEP
RYTLHVVDSPTVKPSRDNHFAIFIIPQGRETEWLFGMDEGRKQLAASAGFRRLITVALHRGQQYESMDHI
QAELSARVMELAPAGMPTQQQVPFLSVGGDIGVRTVQHQDCSPLSGDYVIEDVQGDDKRYFRRLIFLSNR
NVVQSEARLLKDVSHKAQKKRKKDRKKQRPADAEDLPAAPGQSIDKSYLCCEHHKAMIAGLALLRNPELL
LEIPLALLVVGLGGGSLPLFVHDHFPKSCIDAVEIDPSMLEVATQWFGFSQSDRMKVHIADGLDYIASLA
GGGEARPCYDVIMFDVDSKDPTLGMSCPPPAFVEQSFLQKVKSILTPEGVFILNLVCRDLGLKDSVLAGL
KAVFPLLYVRRIEGEVNEILFCQLHPEQKLATPELLETAQALERTLRKPGRGWDDTYVLSDMLKTVKIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015935
ORF Size 2097 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015935.5
RefSeq Size 3421 bp
RefSeq ORF 2100 bp
Locus ID 51603
UniProt ID Q8N6R0
Cytogenetics 1q24.3
Protein Families Druggable Genome
MW 78.8 kDa
Gene Summary Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed:30612740, PubMed:30143613). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed:30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed:30612740, PubMed:30143613). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed:30612740).[UniProtKB/Swiss-Prot Function]

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