KIAA0859 (METTL13) (NM_015935) Human Untagged Clone

CAT#: SC114533

METTL13 (untagged)-Human methyltransferase like 13 (METTL13), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIAA0859
• Synonyms: 5630401D24Rik; CGI-01; DFNB26; DFNB26M; DFNM1; feat; KIAA0859; METTL13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114533 sequence for NM_015935 edited (data generated by NextGen Sequencing)
  ATGAACCTCTTACCTAAAAGTTCCAGGGAGTTTGGCTCCGTTGACTATTGGGAGAAGTTC
  TTCCAGCAGCGAGGAAAGAAAGCTTTCGAGTGGTATGGAACCTACCTGGAACTGTGCGGG
  GTGCTACACAAATATATCAAGCCCAGGGAAAAGGTGCTGGTGATTGGGTGTGGCAACTCA
  GAACTGAGTGAGCAACTGTATGATGTGGGCTATCGGGATATAGTGAACATCGACATCAGT
  GAGGTTGTCATCAAGCAAATGAAGGAATGTAATGCCACCCGACGGCCCCAGATGAGCTTC
  TTGAAGATGGACATGACGCAGATGGAGTTTCCTGATGCCTCGTTCCAGGTGGTGTTGGAC
  AAGGGCACCCTGGATGCTGTCCTGACAGATGAGGAAGAGAAGACCTTACAACAGGTGGAC
  AGGATGCTGGCTGAGGTTGGCCGTGTCCTGCAGGTGGGCGGTCGCTATCTCTGCATCTCC
  CTGGCTCAGGCTCACATCCTGAAGAAAGCAGTGGGCCACTTCTCCCGGGAGGGGTGGATG
  GTGAGGGTGCACCAAGTGGCCAACAGCCAGGACCAGGTGTTGGAAGCAGAGCCTCAGTTC
  TCCTTGCCTGTCTTTGCCTTCATCATGACCAAGTTCAGGCCAGTCCCTGGCTCTGCCCTT
  CAGATCTTTGAGCTGTGTGCTCAGGAGCAGCGCAAGCCTGTGCGGCTGGAGAGTGCCGAG
  CGGCTGGCCGAGGCGGTGCAGGAGCGACAGCAGTATGCCTGGCTGTGCAGCCAGCTGCGC
  CGCAAGGCCAGGCTGGGGAGTGTGTCTCTGGACTTGTGCGATGGGGACACGGGGGAGCCA
  CGCTACACCCTCCACGTGGTGGACAGCCCCACTGTGAAACCATCGCGGGACAATCATTTT
  GCGATTTTCATCATCCCTCAGGGCCGGGAGACCGAGTGGCTCTTTGGCATGGATGAGGGC
  CGGAAACAGCTGGCGGCCAGTGCTGGCTTCAGGAGGTTGATTACAGTGGCCCTTCACCGA
  GGTCAGCAGTATGAAAGCATGGACCACATCCAAGCTGAGCTGTCGGCTAGAGTCATRGAG
  CTGGCCCCAGCTGGGATGCCCACCCAGCAGCAGGTCCCCTTTCTGTCTGTGGGTGGGGAC
  ATTGGGGTCCGGACCGTTCAGCACCAAGACTGCAGCCCCTTGAGCGGTGACTATGTCATT
  GAGGATGTGCAAGGGGATGACAAGCGATACTTCCGTCGACTGATCTTCCTCAGCAACAGG
  AATGTGGTGCAGTCCGAAGCCAGGTTGCTGAAGGATGTGTCTCACAAAGCCCAGAAGAAG
  CGGAAAAAGGACAGGAAGAAGCAGCGGCCTGCTGATGCGGAGGACCTCCCTGCAGCCCCG
  GGGCAGTCCATTGATAAGAGTTACCTGTGTTGTGAACACCACAAAGCCATGATCGCTGGC
  CTTGCCCTGCTGAGAAACCCAGAGCTACTCCTAGAGATCCCACTGGCATTGTTGGTGGTA
  GGCCTGGGCGGGGGCAGCCTCCCCCTCTTTGTCCACGATCATTTTCCAAAGTCCTGCATT
  GATGCTGTGGAGATCGATCCCTCCATGTTGGAAGTGGCCACCCAGTGGTTTGGCTTCTCC
  CAGAGTGACCGAATGAAGGTCCACATTGCAGATGGCCTGGACTATATCGCCAGCTTGGCA
  GGAGGAGGAGAAGCACGGCCTTGCTAYGATGTCATAATGTTTGATGTTGACAGTAAGGAC
  CCAACACTGGGAATGAGTTGTCCGCCCCCAGCATTTGTGGAGCAATCTTTTCTACAGAAG
  GTTAAAAGCATCTTGACTCCTGAAGGTGTTTTTATTCTCAACCTTGTGTGCCGAGACTTG
  GGGCTAAAAGACTCAGTGCTGGCTGGGCTCAAGGCAGTGTTCCCCCTCCTATATGTCCGG
  CGAATTGAGGGTGAAGTGAATGAGATCCTGTTCTGTCAGCTGCACCCTGAGCAAAAACTT
  GCCACACCAGAGCTCCTAGAAACAGCCCAGGCTTTGGAGCGGACCCTGAGGAAGCCTGGG
  AGGGGTTGGGATGACACGTATGTCTTGTCAGATATGCTCAAGACGGTGAAAATTGTGTGA
  
  Clone variation with respect to NM_015935.4
  129 t=>c;1077 g=>r;1707 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015935 unedited
  GTAACTACTGTTTTTATCCACTTTTATAGGCGGCCGCGCAATTCGCACGAGGGGCAAGCG
  TGTGTGAGATTCAGTGGTCCATGCGTGCGTTTGTCGTGTAAGGGTCATTCCTGGGGTTTG
  GAGTGGGGGAACAAATCAATGTGGCTGTTTTTCCGTGGAAAGAATTCCCACTGCAGTGTC
  CCGGAGCCTGCGTGTGGTGGGCAAGCTCCTCAGATGGTATCTCACAGGGAATAGGGGAGT
  CTTGAAAACGCAGCTTCGGCAGTAGGAACATGAACCTCTTACCTAAAAGTTCCAGGGAGT
  TTGGCTCCGTTGACTATTGGGAGAAGTTCTTCCAGCAGCGAGGAAAGAAAGCTTTCGAGT
  GGTATGGAACCTACCTGGAACTGTGCGGGGTGCTACACAAATATATCAAGCCCAGGGAAA
  AGGTGCTGGTGATTGGGTGTGGCAACTCAGAACTGAGTGAGCAACTGTATGATGTGGGCT
  ATCGGGATATAGTGAACATCGACATCAGTGAGGTTGTCATCAAGCAAATGAAGGAATGTA
  ATGCCACCCGACGGCCCCAGATGAGCTTCTTGAAGATGGACATGACGCAGATGGAGTTTC
  CTGATGCCTCGTTCCAGGTGGTGTTGGACAAGGGCACCCTGGATGCTGTCCTGACAGATG
  AGGAAGAGAAGACCTTACAACAGGTGGACAGGATGCTGGCTGAGGTTGGCCGTGTCCTGC
  ANGTGGGCGGTCGCTATCTCTGCATCTCCCTGGCTCANGCTCACATCCTGAAGAAAGCAG
  TGGGCCACTTCTCCNCGGAGGGGTGGATGGTGAGGGTGCACCAAGTGGCCAACAGCCAGG
  ACCAGGTGTTGGAAGCAGAGCCTCAGTTCTTCTTGCCTGTCTTTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015935 unedited
  TGTACGCGGCCGCATTTAGATCGAGTTTTTTTTTTTTTTTTTTGGAAACATTGGTTTAAT
  GGTTCTGAATAAATGGTTAAGATTGATGTTTCCAGCCAAGTGAGATTTGGGTTTAGATAT
  AGTTTGGGCTTAAGTATCCTTTATCTCAGATTAAATGAGACAGTGCATGTAAACCACTTA
  GAAGAATGCCTGGAACACATTAACTTTCATTACTAATGGTTTTGGTCATTCTTAAACATT
  TTCACTTCTGCCACCACCCTTTATGCAGTCTGGCACATAGCTGTCACTCAGCAAATGTGA
  GCAAACAAACACTACAGACTTTAGTCTGCCAAGCTTTTAACCTCCCAAGACACGGGGTCC
  GGCCCATCCACTTCGTATCCTGCCAAATATACCCCTGGCTCTCGCACCCAAGCCCGGGCC
  CCTCCTGACCCCCCCCTACACTTCACCCCGGCCCCCTTCTCCTTCACCAATGTCTCCCCC
  TCCCCCCCCCCCCCTCCTCCCCCCCCCCCCCCTCCCAGTTTCGTTCCGCCTCACGTACAT
  ATCTTATCCTCTCCCATTCCCCTCCTCTTCAATTCCTTTTATTCCACTCCCCCCCTTTCA
  CCCGCCACTTCCGTTAGTATCCTCCGCCTCCCCCTCACACTCTCTCTCCACCTCCCCCTC
  TACCCCTCTCTCCATTCCCCTTTCTCCTCTCTTCCTCCTCCCTGCCGCCCGCCCCGCTCC
  CCTCAACTCCCCCCACCCCATTCTCACCCACACCCACCCCCAACTTTCCTTCTCCCTCCA
  CCCACATCCCTCTCCCTCCTCTCCCTTCTACCCCCCCCTTTTATCAATATTTTCATATCC
  CTCCTTCCTTCCTCCCTCCCCTCCCCCCTCCCTCTCTCCTACCCTTCGGTATAACCTACT
  ACATCGTCTCTCCACACTCTTTTATCTCCATTATAGCACATTTCCCTACCACCTCCCTCT
  TTTTTCCCTCTAACCATATCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015935
• Insert Size: 3330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015935.4, NP_057019.3
• RefSeq Size: 3421 bp
• RefSeq ORF: 2100 bp
• Locus ID: 51603
• UniProt ID: Q8N6R0
• Cytogenetics: 1q24.3
• Protein Families: Druggable Genome
• Gene Summary: Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed:30612740, PubMed:30143613). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed:30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed:30612740, PubMed:30143613). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed:30612740).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).