GPR48 (LGR4) (NM_018490) Human Tagged ORF Clone

CAT#: RC221345

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LGR4 (Myc-DDK-tagged)-Human leucine-rich repeat containing G protein-coupled receptor 4 (LGR4)



  "NM_018490" in other vectors (6)

Reconstitution Protocol

USD 1,306.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LGR4
Synonyms BNMD17; GPR48
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC221345 representing NM_018490
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGCCCGCTAGGGCTGCTCTGCTTCCTCGCCCTGGGGCTGCTCGGCTCGGCCGGGCCCAGCGGCG
CGGCGCCGCCTCTCTGCGCGGCGCCCTGCAGCTGCGACGGCGACCGTCGGGTGGACTGCTCCGGGAAGGG
GCTGACGGCCGTGCCCGAGGGGCTCAGCGCCTTCACCCAAGCGCTGGATATCAGTATGAACAACATTACT
CAGTTGCCAGAAGATGCATTTAAGAACTTTCCTTTTCTAGAAGAGCTACAATTGGCGGGCAACGACCTTT
CTTTTATCCACCCAAAGGCCTTGTCTGGGTTGAAAGAACTCAAAGTTCTAACGCTCCAGAATAATCAGTT
GAAAACAGTACCCAGTGAAGCCATTCGAGGGCTGAGTGCTTTGCAGTCTTTGCGTTTAGATGCCAACCAT
ATTACCTCAGTCCCCGAGGACAGTTTTGAAGGACTTGTTCAGTTACGGCATCTGTGGCTGGATGACAACA
GCTTGACGGAGGTGCCTGTGCACCCCCTCAGCAATCTGCCCACCCTACAGGCGCTGACCCTGGCTCTCAA
CAAGATCTCAAGCATCCCTGACTTTGCATTTACCAACCTTTCAAGCCTGGTAGTTCTGCATCTTCATAAC
AATAAAATTAGAAGCCTGAGTCAACACTGTTTTGATGGACTAGATAACCTGGAGACCTTAGACTTGAATT
ATAATAACTTGGGGGAATTTCCTCAGGCTATTAAAGCCCTTCCTAGCCTTAAAGAGCTAGGATTTCATAG
TAATTCTATTTCTGTTATCCCTGATGGAGCATTTGATGGTAATCCACTCTTAAGAACTATACATTTGTAT
GATAATCCTCTGTCTTTTGTGGGGAACTCAGCATTTCACAATTTATCTGATCTTCATTCCCTAGTCATTC
GTGGTGCAAGCATGGTGCAGCAGTTCCCCAATCTTACAGGAACTGTCCACCTGGAAAGTCTGACTTTGAC
AGGTACAAAGATAAGCAGCATACCTAATAATTTGTGTCAAGAACAAAAGATGCTTAGGACTTTGGACTTG
TCTTACAATAATATAAGAGACCTTCCAAGTTTTAATGGTTGCCATGCTCTGGAAGAAATTTCTTTACAGC
GTAATCAAATCTACCAAATAAAGGAAGGCACCTTTCAAGGCCTGATATCTCTAAGGATTCTAGATCTGAG
TAGAAACCTGATACATGAAATTCACAGTAGAGCTTTTGCCACACTTGGGCCAATAACTAACCTAGATGTA
AGTTTCAATGAATTAACTTCCTTTCCTACGGAAGGCCTGAATGGGCTAAATCAACTGAAACTTGTGGGCA
ACTTCAAGCTGAAAGAAGCCTTAGCAGCAAAAGACTTTGTTAACCTCAGGTCTTTATCAGTACCATATGC
TTATCAGTGCTGTGCATTTTGGGGTTGTGACTCTTATGCAAATTTAAACACAGAAGATAACAGCCTCCAG
GACCACAGTGTGGCACAGGAGAAAGGTACTGCTGATGCAGCAAATGTCACAAGCACTCTTGAAAATGAAG
AACATAGTCAAATAATTATCCATTGTACACCTTCAACAGGTGCTTTTAAGCCCTGTGAATATTTACTGGG
AAGCTGGATGATTCGTCTTACTGTGTGGTTCATTTTCTTGGTTGCATTATTTTTCAACCTGCTTGTTATT
TTAACAACATTTGCATCTTGTACATCACTGCCTTCGTCCAAATTGTTTATAGGCTTGATTTCTGTGTCTA
ACTTATTCATGGGAATCTATACTGGCATCCTAACTTTTCTTGATGCTGTGTCCTGGGGCAGATTCGCTGA
ATTTGGCATTTGGTGGGAAACTGGCAGTGGCTGCAAAGTAGCTGGGTTTCTTGCAGTTTTCTCCTCAGAA
AGTGCCATATTTTTATTAATGCTAGCAACTGTCGAAAGAAGCTTATCTGCAAAAGATATAATGAAAAATG
GGAAGAGCAATCATCTCAAACAGTTCCGGGTTGCTGCCCTTTTGGCTTTCCTAGGTGCTACAGTAGCAGG
CTGTTTTCCCCTTTTCCATAGAGGGGAATATTCTGCATCACCCCTTTGTTTGCCATTTCCTACAGGTGAA
ACGCCATCATTAGGATTCACTGTAACGTTAGTGCTATTAAACTCACTAGCATTTTTATTAATGGCCGTTA
TCTACACTAAGCTATACTGCAACTTGGAAAAAGAGGACCTCTCAGAAAACTCACAATCTAGCATGATTAA
GCATGTCGCTTGGCTAATCTTCACCAATTGCATCTTTTTCTGCCCTGTGGCGTTTTTTTCATTTGCACCA
TTGATCACTGCAATCTCTATCAGCCCCGAAATAATGAAGTCTGTTACTCTGATATTTTTTCCATTGCCTG
CTTGCCTGAATCCAGTCCTGTATGTTTTCTTCAACCCAAAGTTTAAAGAAGACTGGAAGTTACTGAAGCG
ACGTGTTACCAAGAAAAGTGGATCAGTTTCAGTTTCCATCAGTAGCCAAGGTGGTTGTCTGGAACAGGAT
TTCTACTACGACTGTGGCATGTACTCACATTTGCAGGGCAACCTGACTGTTTGCGACTGCTGCGAATCGT
TTCTTTTAACAAAGCCAGTATCATGCAAACACTTGATAAAATCACACAGCTGTCCTGCATTGGCAGTGGC
TTCTTGCCAAAGACCTGAGGGCTACTGGTCCGACTGTGGCACACAGTCGGCCCACTCTGATTATGCAGAT
GAAGAAGATTCCTTTGTCTCAGACAGTTCTGACCAGGTGCAGGCCTGTGGACGAGCCTGCTTCTACCAGA
GTAGAGGATTCCCTTTGGTGCGCTATGCTTACAATCTACCAAGAGTTAAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC221345 representing NM_018490
Red=Cloning site Green=Tags(s)

MPGPLGLLCFLALGLLGSAGPSGAAPPLCAAPCSCDGDRRVDCSGKGLTAVPEGLSAFTQALDISMNNIT
QLPEDAFKNFPFLEELQLAGNDLSFIHPKALSGLKELKVLTLQNNQLKTVPSEAIRGLSALQSLRLDANH
ITSVPEDSFEGLVQLRHLWLDDNSLTEVPVHPLSNLPTLQALTLALNKISSIPDFAFTNLSSLVVLHLHN
NKIRSLSQHCFDGLDNLETLDLNYNNLGEFPQAIKALPSLKELGFHSNSISVIPDGAFDGNPLLRTIHLY
DNPLSFVGNSAFHNLSDLHSLVIRGASMVQQFPNLTGTVHLESLTLTGTKISSIPNNLCQEQKMLRTLDL
SYNNIRDLPSFNGCHALEEISLQRNQIYQIKEGTFQGLISLRILDLSRNLIHEIHSRAFATLGPITNLDV
SFNELTSFPTEGLNGLNQLKLVGNFKLKEALAAKDFVNLRSLSVPYAYQCCAFWGCDSYANLNTEDNSLQ
DHSVAQEKGTADAANVTSTLENEEHSQIIIHCTPSTGAFKPCEYLLGSWMIRLTVWFIFLVALFFNLLVI
LTTFASCTSLPSSKLFIGLISVSNLFMGIYTGILTFLDAVSWGRFAEFGIWWETGSGCKVAGFLAVFSSE
SAIFLLMLATVERSLSAKDIMKNGKSNHLKQFRVAALLAFLGATVAGCFPLFHRGEYSASPLCLPFPTGE
TPSLGFTVTLVLLNSLAFLLMAVIYTKLYCNLEKEDLSENSQSSMIKHVAWLIFTNCIFFCPVAFFSFAP
LITAISISPEIMKSVTLIFFPLPACLNPVLYVFFNPKFKEDWKLLKRRVTKKSGSVSVSISSQGGCLEQD
FYYDCGMYSHLQGNLTVCDCCESFLLTKPVSCKHLIKSHSCPALAVASCQRPEGYWSDCGTQSAHSDYAD
EEDSFVSDSSDQVQACGRACFYQSRGFPLVRYAYNLPRVKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018490
ORF Size 2853 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018490.4
RefSeq Size 5211 bp
RefSeq ORF 2856 bp
Locus ID 55366
UniProt ID Q9BXB1
Cytogenetics 11p14.1
Domains 7tm_1, LRRNT, LRR, LRR_TYP, LRR_BAC, LRR_PS
Protein Families Druggable Genome, GPCR, Transmembrane
MW 104.3 kDa
Gene Summary The protein encoded by this gene is a G-protein coupled receptor that binds R-spondins and activates the Wnt signaling pathway. This Wnt signaling pathway activation is necessary for proper development of many organs of the body. [provided by RefSeq, Oct 2016]

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