LPCAT1 (NM_024830) Human Tagged ORF Clone

CAT#: RC220083

LPCAT1 (Myc-DDK-tagged)-Human lysophosphatidylcholine acyltransferase 1 (LPCAT1)

Product Images

Western blot validation of overexpression lysate (Cat# LY403037) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220083 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LPCAT1 protein (Cat# TP320083). The protein was produced from HEK293T cells transfected with LPCAT1 cDNA clone (Cat# RC220083) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LPCAT1
• Synonyms: AGPAT9; AGPAT10; AYTL2; lpcat; LPCAT-1; lysoPAFAT; PFAAP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220083 representing NM_024830
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTGCGGGGATGCGGACCCCGGGCCGCCCCTGCCTCCAGCGCAGGGGCCAGCGACGCTCGGCTGC
  TGGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGCCTCAGCGCCCTGCAGAAGGCCCAGGTGGC
  CCTCATGACACTGACGCTCTTCCCGGTCCGGCTCCTGGTTGCCGCTGCCATGATGCTGCTGGCCTGGCCC
  CTCGCACTTGTCGCATCCCTGGGCTCTGCGGAGAAGGAACCCGAGCAGCCCCCGGCCCTGTGGAGGAAGG
  TTGTGGACTTCCTGCTGAAGGCCATCATGCGCACCATGTGGTTCGCCGGCGGCTTCCACCGGGTGGCCGT
  GAAGGGGCGGCAGGCGCTGCCCACCGAGGCGGCCATCCTCACGCTCGCGCCTCACTCGTCCTACTTCGAC
  GCCATCCCTGTGACCATGACGATGTCCTCCATCGTGATGAAGGCAGAGAGCAGAGACATCCCGATCTGGG
  GAACTCTGATCCAGTATATACGGCCTGTGTTCGTGTCCCGGTCAGACCAGGATTCTCGCAGGAAAACAGT
  AGAAGAAATCAAGAGACGGGCGCAGTCCAACGGAAAGTGGCCACAGATAATGATTTTTCCAGAAGGAACT
  TGTACAAACAGGACCTGCCTAATTACCTTCAAACCTGGTGCATTCATCCCTGGAGCGCCCGTCCAGCCTG
  TGGTTTTACGATATCCAAATAAACTGGACACCATCACATGGACGTGGCAAGGACCTGGAGCGCTGGAAAT
  CCTGTGGCTCACGCTGTGTCAGTTTCACAACCAAGTGGAAATCGAGTTCCTTCCTGTGTACAGCCCTTCT
  GAGGAGGAGAAGAGGAACCCCGCGCTGTATGCCAGCAACGTGCGGCGAGTCATGGCCGAGGCCTTGGGTG
  TCTCCGTGACTGACTACACGTTCGAGGACTGCCAGCTGGCCCTGGCGGAAGGACAGCTCCGTCTCCCCGC
  TGACACTTGCCTTTTAGAATTTGCCAGGCTCGTGCGGGGCCTCGGGCTAAAACCAGAAAAGCTTGAAAAA
  GATCTGGACAGATACTCAGAAAGAGCCAGGATGAAGGGAGGAGAGAAGATAGGTATTGCGGAGTTTGCCG
  CCTCCCTGGAAGTCCCCGTTTCTGACTTGCTGGAAGACATGTTTTCACTGTTCGACGAGAGCGGCAGCGG
  CGAGGTGGACCTGCGAGAGTGTGTGGTTGCCCTGTCTGTCGTCTGCCGGCCGGCCCGGACCCTGGACACC
  ATCCAGCTGGCTTTCAAGATGTACGGAGCGCAAGAGGACGGCAGCGTCGGCGAAGGTGACCTGTCCTGCA
  TCCTCAAGACGGCCCTGGGGGTGGCAGAGCTCACCGTGACCGACCTATTCCGAGCCATTGACCAAGAGGA
  GAAGGGGAAGATCACATTCGCTGACTTCCACAGGTTTGCAGAAATGTACCCTGCCTTCGCAGAGGAATAC
  CTGTACCCGGATCAGACACATTTCGAAAGCTGTGCAGAGACCTCACCTGCGCCAATCCCAAACGGCTTCT
  GTGCCGATTTCAGCCCGGAAAACTCAGACGCTGGGCGGAAGCCTGTTCGCAAGAAGCTGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220083 representing NM_024830
  Red=Cloning site Green=Tags(s)
  
  MRLRGCGPRAAPASSAGASDARLLAPPGRNPFVHELRLSALQKAQVALMTLTLFPVRLLVAAAMMLLAWP
  LALVASLGSAEKEPEQPPALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFD
  AIPVTMTMSSIVMKAESRDIPIWGTLIQYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGT
  CTNRTCLITFKPGAFIPGAPVQPVVLRYPNKLDTITWTWQGPGALEILWLTLCQFHNQVEIEFLPVYSPS
  EEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPEKLEK
  DLDRYSERARMKGGEKIGIAEFAASLEVPVSDLLEDMFSLFDESGSGEVDLRECVVALSVVCRPARTLDT
  IQLAFKMYGAQEDGSVGEGDLSCILKTALGVAELTVTDLFRAIDQEEKGKITFADFHRFAEMYPAFAEEY
  LYPDQTHFESCAETSPAPIPNGFCADFSPENSDAGRKPVRKKLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024830
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024830.5
• RefSeq Size: 3994 bp
• RefSeq ORF: 1605 bp
• Locus ID: 79888
• UniProt ID: Q8NF37
• Cytogenetics: 5p15.33
• Domains: EFh, Acyltransferase
• Protein Families: Transmembrane
• MW: 59 kDa
• Gene Summary: This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers. [provided by RefSeq, Sep 2016]