LPCAT1 (NM_024830) Human Tagged ORF Clone

CAT#: RC220083

  • TrueORF®

LPCAT1 (Myc-DDK-tagged)-Human lysophosphatidylcholine acyltransferase 1 (LPCAT1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_024830" in other vectors (6)

Reconstitution Protocol

USD 497.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00


LPCAT1 Rabbit polyclonal Antibody
    • 100 ul

USD 365.00

Other products for "LPCAT1"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LPCAT1
Synonyms AGPAT9; AGPAT10; AYTL2; lpcat; LPCAT-1; lysoPAFAT; PFAAP3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC220083 representing NM_024830
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCTGCGGGGATGCGGACCCCGGGCCGCCCCTGCCTCCAGCGCAGGGGCCAGCGACGCTCGGCTGC
TGGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGCCTCAGCGCCCTGCAGAAGGCCCAGGTGGC
CCTCATGACACTGACGCTCTTCCCGGTCCGGCTCCTGGTTGCCGCTGCCATGATGCTGCTGGCCTGGCCC
CTCGCACTTGTCGCATCCCTGGGCTCTGCGGAGAAGGAACCCGAGCAGCCCCCGGCCCTGTGGAGGAAGG
TTGTGGACTTCCTGCTGAAGGCCATCATGCGCACCATGTGGTTCGCCGGCGGCTTCCACCGGGTGGCCGT
GAAGGGGCGGCAGGCGCTGCCCACCGAGGCGGCCATCCTCACGCTCGCGCCTCACTCGTCCTACTTCGAC
GCCATCCCTGTGACCATGACGATGTCCTCCATCGTGATGAAGGCAGAGAGCAGAGACATCCCGATCTGGG
GAACTCTGATCCAGTATATACGGCCTGTGTTCGTGTCCCGGTCAGACCAGGATTCTCGCAGGAAAACAGT
AGAAGAAATCAAGAGACGGGCGCAGTCCAACGGAAAGTGGCCACAGATAATGATTTTTCCAGAAGGAACT
TGTACAAACAGGACCTGCCTAATTACCTTCAAACCTGGTGCATTCATCCCTGGAGCGCCCGTCCAGCCTG
TGGTTTTACGATATCCAAATAAACTGGACACCATCACATGGACGTGGCAAGGACCTGGAGCGCTGGAAAT
CCTGTGGCTCACGCTGTGTCAGTTTCACAACCAAGTGGAAATCGAGTTCCTTCCTGTGTACAGCCCTTCT
GAGGAGGAGAAGAGGAACCCCGCGCTGTATGCCAGCAACGTGCGGCGAGTCATGGCCGAGGCCTTGGGTG
TCTCCGTGACTGACTACACGTTCGAGGACTGCCAGCTGGCCCTGGCGGAAGGACAGCTCCGTCTCCCCGC
TGACACTTGCCTTTTAGAATTTGCCAGGCTCGTGCGGGGCCTCGGGCTAAAACCAGAAAAGCTTGAAAAA
GATCTGGACAGATACTCAGAAAGAGCCAGGATGAAGGGAGGAGAGAAGATAGGTATTGCGGAGTTTGCCG
CCTCCCTGGAAGTCCCCGTTTCTGACTTGCTGGAAGACATGTTTTCACTGTTCGACGAGAGCGGCAGCGG
CGAGGTGGACCTGCGAGAGTGTGTGGTTGCCCTGTCTGTCGTCTGCCGGCCGGCCCGGACCCTGGACACC
ATCCAGCTGGCTTTCAAGATGTACGGAGCGCAAGAGGACGGCAGCGTCGGCGAAGGTGACCTGTCCTGCA
TCCTCAAGACGGCCCTGGGGGTGGCAGAGCTCACCGTGACCGACCTATTCCGAGCCATTGACCAAGAGGA
GAAGGGGAAGATCACATTCGCTGACTTCCACAGGTTTGCAGAAATGTACCCTGCCTTCGCAGAGGAATAC
CTGTACCCGGATCAGACACATTTCGAAAGCTGTGCAGAGACCTCACCTGCGCCAATCCCAAACGGCTTCT
GTGCCGATTTCAGCCCGGAAAACTCAGACGCTGGGCGGAAGCCTGTTCGCAAGAAGCTGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC220083 representing NM_024830
Red=Cloning site Green=Tags(s)

MRLRGCGPRAAPASSAGASDARLLAPPGRNPFVHELRLSALQKAQVALMTLTLFPVRLLVAAAMMLLAWP
LALVASLGSAEKEPEQPPALWRKVVDFLLKAIMRTMWFAGGFHRVAVKGRQALPTEAAILTLAPHSSYFD
AIPVTMTMSSIVMKAESRDIPIWGTLIQYIRPVFVSRSDQDSRRKTVEEIKRRAQSNGKWPQIMIFPEGT
CTNRTCLITFKPGAFIPGAPVQPVVLRYPNKLDTITWTWQGPGALEILWLTLCQFHNQVEIEFLPVYSPS
EEEKRNPALYASNVRRVMAEALGVSVTDYTFEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPEKLEK
DLDRYSERARMKGGEKIGIAEFAASLEVPVSDLLEDMFSLFDESGSGEVDLRECVVALSVVCRPARTLDT
IQLAFKMYGAQEDGSVGEGDLSCILKTALGVAELTVTDLFRAIDQEEKGKITFADFHRFAEMYPAFAEEY
LYPDQTHFESCAETSPAPIPNGFCADFSPENSDAGRKPVRKKLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024830
ORF Size 1602 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024830.5
RefSeq Size 3994 bp
RefSeq ORF 1605 bp
Locus ID 79888
UniProt ID Q8NF37
Cytogenetics 5p15.33
Domains EFh, Acyltransferase
Protein Families Transmembrane
MW 59 kDa
Gene Summary This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers. [provided by RefSeq, Sep 2016]

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.