LPCAT1 (NM_024830) Human Untagged Clone

CAT#: SC128138

LPCAT1 (untagged)-Human lysophosphatidylcholine acyltransferase 1 (LPCAT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LPCAT1
• Synonyms: AGPAT9; AGPAT10; AYTL2; lpcat; LPCAT-1; lysoPAFAT; PFAAP3
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_024830, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGGCTGCGGGGATGCGGACCCCGGGCCGCCCCTGCCTCCAGCGCAGGGGCCAGCGACGCTCGGCTGC
  TGGCGCCCCCGGGGCGGAACCCCTTCGTGCACGAGCTGCGCCTCAGCGCCCTGCAGAAGGCCCAGGTGGC
  CCTCATGACACTGACGCTCTTCCCGGTCCGGCTCCTGGTTGCCGCTGCCATGATGCTGCTGGCCTGGCCC
  CTCGCACTTGTCGCATCCCTGGGCTCTGCGGAGAAGGAACCCGAGCAGCCCCCGGCCCTGTGGAGGAAGG
  TTGTGGACTTCCTGCTGAAGGCCATCATGCGCACCATGTGGTTCGCCGGCGGCTTCCACCGGGTGGCCGT
  GAAGGGGCGGCAGGCGCTGCCCACCGAGGCGGCCATCCTCACGCTCGCGCCTCACTCGTCCTACTTCGAC
  GCCATCCCTGTGACCATGACGATGTCCTCCATCGTGATGAAGGCAGAGAGCAGAGACATCCCGATCTGGG
  GAACTCTGATCCAGTATATACGGCCTGTGTTCGTGTCCCGGTCAGACCAGGATTCTCGCAGGAAAACAGT
  AGAAGAAATCAAGAGACGGGCGCAGTCCAACGGAAAGTGGCCACAGATAATGATTTTTCCAGAAGGAACT
  TGTACAAACAGGACCTGCCTAATTACCTTCAAACCTGGTGCATTCATCCCTGGAGCGCCCGTCCAGCCTG
  TGGTTTTACGATATCCAAATAAACTGGACACCATCACATGGACGTGGCAAGGACCTGGAGCGCTGGAAAT
  CCTGTGGCTCACGCTGTGTCAGTTTCACAACCAAGTGGAAATCGAGTTCCTTCCTGTGTACAGCCCTTCT
  GAGGAGGAGAAGAGGAACCCCGCGCTGTATGCCAGCAACGTGCGGCGAGTCATGGCCGAGGCCTTGGGTG
  TCTCCGTGACTGACTACACGTTCGAGGACTGCCAGCTGGCCCTGGCGGAAGGACAGCTCCGTCTCCCCGC
  TGACACTTGCCTTTTAGAATTTGCCAGGCTCGTGCGGGGCCTCGGGCTAAAACCAGAAAAGCTTGAAAAA
  GATCTGGACAGATACTCAGAAAGAGCCAGGATGAAGGGAGGAGAGAAGATAGGTATTGCGGAGTTTGCCG
  CCTCCCTGGAAGTCCCCGTTTCTGACTTGCTGGAAGACATGTTTTCACTGTTCGACGAGAGCGGCAGCGG
  CGAGGTGGACCTGCGAGAGTGTGTGGTTGCCCTGTCTGTCGTCTGCCGGCCGGCCCGGACCCTGGACACC
  ATCCAGCTGGCTTTCAAGATGTACGGAGCGCAAGAGGACGGCAGCGTCGGCGAAGGTGACCTGTCCTGCA
  TCCTCAAGACGGCCCTGGGGGTGGCAGAGCTCACCGTGACCGACCTATTCCGAGCCATTGACCAAGAGGA
  GAAGGGGAAGATCACATTCGCTGACTTCCACAGGTTTGCAGAAATGTACCCTGCCTTCGCAGAGGAATAC
  CTGTACCCGGATCAGACACATTTCGAAAGCTGTGCAGAGACCTCACCTGCGCCAATCCCAAACGGCTTCT
  GTGCCGATTTCAGCCCGGAAAACTCAGACGCTGGGCGGAAGCCTGTTCGCAAGAAGCTGGATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024830 unedited
  GGGGGGNNNGGGGGGANNNNNNNNNNTTTNNNNNNNNTTTTTTCCCCCGCCCGTTGNCGC
  AAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCTCATTTAGGTGACAC
  TATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCGCCGC
  GCATCCTCGGCCCGCGCCCCGAGACCCGCGCCCAGCTAGCCCCGGCCCCGCTCGGCGCCC
  CAGGCAGCTCGGCTGCGCTCGCCGCGGGACGGCGCGGCCATGAGGCTGCGGCGATGCGGA
  CCCCGGGCCGCCCCTGCCTCCAGCGCAGGGGCCAGCGACGCTCGGCTGCTGGCGCCCCCG
  GGGCGGAACCCCTTCGTGCACGAGCTGCGCCTCAGCGCCCTGCAGAAGGCCCAGGTGGCC
  CTCATGACACTGACGCTCTTCCCGGTCCGGCTCCTGGTTGCCGCTGCCATGATGCTGCTG
  GCCTGGCCCCTCGCACTTGTCGCATCCCTGGGCTCTGCGGAGAAGGAACCCGAGCAGCCC
  CCGGCCCTGTGGAGGAAGGTTGTGGACTTCCTGCTGAAGGCCATCATGCGCACCATGTGG
  TTCGCCGGCGGCTTCCACCGGGTGGCCGTGAAGGGGCGGCAGGCGCTGCCCACCGAGGCG
  GNCATNCTCACGCTCGCGCCTCACTCGTNCTACTTCGACGCCATCCCTGTGACCATGACG
  ATGTCCTCCATCGTGATGAAGGCAGAGAGCAGAGACATCCCGATCTGGGGAACTCTGATC
  CAGTATATACGGCCTGTGTTCGTGTCCCGGTCAGACCAGNATTCTCGCAGGAAAACAGTN
  GAAAGAAATCAGAGACGGGCGCAGTCCAACGGGAAGTGGCCACAGATAATGATTTTTCCA
  GAAGGACCTGTACAACAGGACCTGNCTAATACCTTCAACCTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024830 unedited
  NNNAACCATTAANCTTTTAGTTTTCATTATTCCATGNAAAATCCAGTTCCTTCAGGCAAC
  CCTCCCTCTGCCCTCAACCCCACAGTCCCCGCAGCCAGGGCCCTGCCCCACCTGCCCACG
  GGTCTGGCCCCCAGGCCACCAGGGGCCCGCTGCGTCCTGTCTCACACACAAGGGGTTTAA
  CTCAACGCTATGTACATTCACAGTTCCGAATATCCGCCAACTCTAAGTCGCCACGAAGAG
  AAAAGAGAATCAGGAGGAACAAACATCCATTCAAAGTCTGTTTATCAGAGATTTTTTTTT
  CTGAAAATGCACAGTGGCATTTCATTCAAAAAACCCTTCATCTGCAGACCTGCGGAAGGG
  AGGTGGCCTGGGTCCCTTCCCTCGGAATATCTTAGTTTATTTACCTCCTTCATTGGCCAT
  TTGCAGGCTCTTCTCCTTGAAAAATGAATCTTTACGCATTCTCCAATTATAAAATCAGTG
  ACTGTTAGCTACCAAAGGCTGCACTAGGATTTCTTCTGTGTCCAACACGCCAAGAGCCCT
  GAAATTGACTTCGGTTTACTCCATCCCTGTCTGTCCTGCTGGCAGTGTCCATGCTAAAAA
  ACAAGTCGAGGTGATTGATTGAAACGGAGCTGAAGGTTGTTTTTAAATGTCTGTCAGTGG
  AGAAACGCGTATCACGAATCTCTGAGGAAGTTAGTAAACATTTTTTCCCGTACTTACTGG
  CCTTGGTGGTCACTTTCTATAGAGATGCCCCANATATAAAAATCAATTCATTTCAGANAT
  CAAAAAAATTTCCAAACAAACCCGGAGCCTTTGCTTTANGAAGCAAACTCAAGTCTGTGA
  TGATTATTGTGCTTGAAGGATGGGTTTCTAATTCTGTCATANAAAATGATGAAACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024830
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024830.3, NP_079106.3
• RefSeq Size: 3994 bp
• RefSeq ORF: 1605 bp
• Locus ID: 79888
• UniProt ID: Q8NF37
• Cytogenetics: 5p15.33
• Domains: EFh, Acyltransferase
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family of proteins. The encoded enzyme plays a role in phospholipid metabolism, specifically in the conversion of lysophosphatidylcholine to phosphatidylcholine in the presence of acyl-CoA. This process is important in the synthesis of lung surfactant and platelet-activating factor (PAF). Elevated expression of this gene may contribute to the progression of oral squamous cell, prostate, breast, and other human cancers. [provided by RefSeq, Sep 2016]