Staufen (STAU1) (NM_017452) Human Tagged ORF Clone

CAT#: RC218095

STAU1 (Myc-DDK-tagged)-Human staufen, RNA binding protein, homolog 1 (Drosophila) (STAU1), transcript variant T2

Product Images

Circular map for RC218095

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAU1 (Cat# RC218095, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAU1 (Cat# TA811475)(1:2000). Positive lysates LY429536 (100ug) and LC429536 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY413756) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218095 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Staufen
• Synonyms: PPP1R150; STAU
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218095 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACTTGGAAAAAAACCAATGTATAAGCCTGTTGACCCTTACTCTCGGATGCAGTCCACCTATAACT
  ACAACATGAGAGGAGGTGCTTATCCCCCGAGGTACTTTTACCCATTTCCAGTTCCACCTTTACTTTATCA
  AGTGGAACTTTCTGTGGGAGGACAGCAATTTAATGGCAAAGGAAAGACAAGACAGGCTGCGAAACACGAT
  GCTGCTGCCAAAGCGTTGAGGATCCTGCAGAATGAGCCCCTGCCAGAGAGGCTGGAGGTGAATGGAAGAG
  AATCCGAAGAAGAAAATCTCAATAAATCTGAAATAAGTCAAGTGTTTGAGATTGCACTTAAACGGAACTT
  GCCTGTGAATTTCGAGGTGGCCCGGGAGAGTGGCCCACCCCACATGAAGAACTTTGTGACCAAGGTTTCG
  GTTGGGGAGTTTGTGGGGGAAGGTGAAGGGAAAAGCAAGAAGATTTCAAAGAAAAATGCCGCCATAGCTG
  TTCTTGAGGAGCTGAAGAAGTTACCGCCCCTGCCTGCAGTTGAACGAGTAAAGCCTAGAATCAAAAAGAA
  AACAAAACCCATAGTCAAGCCACAGACAAGCCCAGAATATGGCCAGGGGATCAATCCGATTAGCCGACTG
  GCCCAGATCCAGCAGGCAAAAAAGGAGAAGGAGCCAGAGTACACGCTCCTCACAGAGCGAGGCCTCCCGC
  GCCGCAGGGAGTTTGTGATGCAGGTGAAGGTTGGAAACCACACTGCAGAAGGAACGGGCACCAACAAGAA
  GGTGGCCAAGCGCAATGCAGCCGAGAACATGCTGGAGATCCTTGGTTTCAAAGTCCCGCAGGCGCAGCCC
  ACCAAACCCGCACTCAAGTCAGAGGAGAAGACACCCATAAAGAAACCAGGGGATGGAAGAAAAGTAACCT
  TTTTTGAACCTGGCTCTGGGGATGAAAATGGGACTAGTAATAAAGAGGATGAGTTCAGGATGCCTTATCT
  AAGTCATCAGCAGCTGCCTGCTGGAATTCTTCCCATGGTGCCCGAGGTCGCCCAGGCTGTAGGAGTTAGT
  CAAGGACATCACACCAAAGATTTTACCAGGGCAGCTCCGAATCCTGCCAAGGCCACGGTAACTGCCATGA
  TAGCCCGAGAGTTGTTGTATGGGGGCACCTCGCCCACAGCCGAGACCATTTTAAAGAATAACATCTCTTC
  AGGCCACGTACCCCATGGACCTCTCACGAGACCCTCTGAGCAACTGGACTATCTTTCCAGAGTCCAGGGA
  TTCCAGGTTGAATACAAAGACTTCCCCAAAAACAACAAGAACGAATTTGTATCTCTTATCAATTGCTCCT
  CTCAGCCACCTCTGATCAGCCATGGTATCGGCAAGGATGTGGAGTCCTGCCATGATATGGCTGCGCTGAA
  CATCTTAAAGTTGCTGTCTGAGTTGGACCAACAAAGTACAGAGATGCCAAGAACAGGAAACGGACCAATG
  TCTGTGTGTGGGAGGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218095 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPFPVPPLLYQVELSVGGQQFNGKGKTRQAAKHD
  AAAKALRILQNEPLPERLEVNGRESEEENLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVS
  VGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRL
  AQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQP
  TKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPMVPEVAQAVGVS
  QGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGHVPHGPLTRPSEQLDYLSRVQG
  FQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVESCHDMAALNILKLLSELDQQSTEMPRTGNGPM
  SVCGRC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017452
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017452.3
• RefSeq Size: 3399 bp
• RefSeq ORF: 1491 bp
• Locus ID: 6780
• UniProt ID: O95793
• Cytogenetics: 20q13.13
• Domains: DSRM
• MW: 54.9 kDa
• Gene Summary: Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. [provided by RefSeq, Apr 2020]