METTL21C (NM_001010977) Human Tagged ORF Clone

CAT#: RC217219

METTL21C (Myc-DDK-tagged)-Human methyltransferase like 21C (METTL21C)

Product Images

Western blot validation of overexpression lysate (Cat# LY423250) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217219 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified METTL21C protein (Cat# TP317219). The protein was produced from HEK293T cells transfected with METTL21C cDNA clone (Cat# RC217219) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: METTL21C
• Synonyms: C13orf39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217219 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGTGTCTGAGCTCCGCGCAGCAGCCTGGGCGCCGGGGGGAAGGACTCAGCTCCCCGGGTGGCT
  GGTTAGAGGCTGAGAAGAAGGGGGCTCCGCAGAAAGACAGCACCGGGGGAGTCCTAGAAGAATCCAACAA
  GATAGAACCATCTCTTCATAGCCTCCAGAAATTTGTTCCTACAGATTACGCCAGCTACACTCAGGAGCAT
  TATCGGTTTGCAGGAAAGGAGATTGTCATCCAGGAATCCATAGAGAGTTACGGAGCGGTGGTGTGGCCAG
  GGGCTATGGCTTTGTGTCAATACTTGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAAATACT
  TGAAATTGGTGCCGGACCAGGCCTTGTTTCCATTGTGGCCAGTATTTTAGGAGCTCAAGTCACAGCAACA
  GATTTGCCTGATGTCCTGGGAAACCTTCAATACAATCTTTTAAAAAACACACTACAATGTACAGCACATC
  TGCCTGAAGTGAAAGAACTGGTATGGGGGGAAGACCTGGACAAAAACTTTCCCAAGTCAGCTTTTTACTA
  TGATTACGTCCTAGCCTCAGATGTGGTCTACCATCACTACTTCCTGGACAAGCTGCTCACCACCATGGTG
  TACCTTTCCCAGCCAGGGACGGTGCTGCTTTGGGCAAACAAATTCAGGTTCAGCACCGACTATGAATTTT
  TAGATAAATTCAAGCAAGTTTTTGACACAACACTGTTGGCTGAATATCCAGAGTCATCAGTCAAACTTTT
  TAAGGGGATACTAAAATGGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217219 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDVCLSSAQQPGRRGEGLSSPGGWLEAEKKGAPQKDSTGGVLEESNKIEPSLHSLQKFVPTDYASYTQEH
  YRFAGKEIVIQESIESYGAVVWPGAMALCQYLEEHAEELNFQDAKILEIGAGPGLVSIVASILGAQVTAT
  DLPDVLGNLQYNLLKNTLQCTAHLPEVKELVWGEDLDKNFPKSAFYYDYVLASDVVYHHYFLDKLLTTMV
  YLSQPGTVLLWANKFRFSTDYEFLDKFKQVFDTTLLAEYPESSVKLFKGILKWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010977
• ORF Size: 792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010977.2, NP_001010977.1
• RefSeq Size: 1115 bp
• RefSeq ORF: 795 bp
• Locus ID: 196541
• UniProt ID: Q5VZV1
• Cytogenetics: 13q33.1
• Protein Families: Druggable Genome
• MW: 29.6 kDa
• Gene Summary: Protein-lysine methyltransferase.[UniProtKB/Swiss-Prot Function]