METTL21C (NM_001010977) Human Untagged Clone

CAT#: SC301552

METTL21C (untagged)-Human methyltransferase like 21C (METTL21C)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: METTL21C
• Synonyms: C13orf39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC301552 representing NM_001010977.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACGTGTGTCTGAGCTCCGCGCAGCAGCCTGGGCGCCGGGGGGAAGGACTCAGCTCCCCGGGTGGC
  TGGTTAGAGGCTGAGAAGAAGGGGGCTCCGCAGAAAGACAGCACCGGGGGAGTCCTAGAAGAATCCAAC
  AAGATAGAACCATCTCTTCATAGCCTCCAGAAATTTGTTCCTACAGATTACGCCAGCTACACTCAGGAG
  CATTATCGGTTTGCAGGAAAGGAGATTGTCATCCAGGAATCCATAGAGAGTTACGGAGCGGTGGTGTGG
  CCAGGGGCTATGGCTTTGTGTCAATACTTGGAGGAACATGCCGAGGAATTGAATTTCCAAGATGCAAAA
  ATACTTGAAATTGGTGCCGGACCAGGCCTTGTTTCCATTGTGGCCAGTATTTTAGGAGCTCAAGTCACA
  GCAACAGATTTGCCTGATGTCCTGGGAAACCTTCAATACAATCTTTTAAAAAACACACTACAATGTACA
  GCACATCTGCCTGAAGTGAAAGAACTGGTATGGGGGGAAGACCTGGACAAAAACTTTCCCAAGTCAGCT
  TTTTACTATGATTACGTCCTAGCCTCAGATGTGGTCTACCATCACTACTTCCTGGACAAGCTGCTCACC
  ACCATGGTGTACCTTTCCCAGCCAGGGACGGTGCTGCTTTGGGCAAACAAATTCAGGTTCAGCACCGAC
  TATGAATTTTTAGATAAATTCAAGCAAGTTTTTGACACAACACTGTTGGCTGAATATCCAGAGTCATCA
  GTCAAACTTTTTAAGGGGATACTAAAATGGGACTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001010977
• Insert Size: 795 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001010977.2
• RefSeq Size: 1115 bp
• RefSeq ORF: 795 bp
• Locus ID: 196541
• UniProt ID: Q5VZV1
• Cytogenetics: 13q33.1
• Protein Families: Druggable Genome
• MW: 29.6 kDa
• Gene Summary: Protein-lysine methyltransferase.[UniProtKB/Swiss-Prot Function]