AFMID (NM_001010982) Human Tagged ORF Clone
CAT#: RC217126
AFMID (Myc-DDK-tagged)-Human arylformamidase (AFMID), transcript variant 2
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001010982" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | AFMID |
Synonyms | FKF; KF; KFA |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC217126 representing NM_001010982
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGATGTGTCTGGTGTGGGTTTCCCAAGCAAGGTTCCTTGGAAGAAGATGTCTGCAGAGGAGCTGG AGAATCAGTACTGTCCCAGCCGATGGGTTGTCCGACTGGGAGCAGAGGAAGCCTTGAGGACCTACTCACA GATAGGAATTGAAGCCACCACAAGGGCCCGGGCCACCAGGAAGAGCCTGCTGCATGTCCCCTATGGAGAC GGCGAAGGGGAGAAAGTGGACATTTACTTCCCCGACGAGTCGTCTGAAGCCTTGCCTTTCTTCCTGTTCT TTCACGGAGGATACTGGCAGAGCGGAAGTAAGGATGAGTCTGCCTTCATGGTCCACCCGCTGACGGCACA GGGAGTGGCCGTGGTAATAGTGGCTTACGGCATCGCCCCCAAAGGCACCCTGGACCACATGGTAGACCAG GTGACCCGCAGCGTTGCGTTTGTCCAGAAGCGGTATCCAAGCAACAAGGGAATTTACCTGTGTGGACACT CAGCCGGGGCCCACCTGGCTGCCATGATGCTCCTGGCCGACTGGACCAAGCATGGGGTCACGCCCAACCT CAGAGGCTTTTTCCTGGTGAGTGGGGTCTTTGACCTGGAGCCCATCGTGTATACTTCACAGAACGTTGCT CTCCAGCTGACCCTGGAGGACGCTCAGAGGAATAGCCCCCAGCTGAAGGTGGCCCAGGCACAGCCGGTGG ACCCCACCTGCCGTGTGCTGGTGGTCGTGGGCCAGTTCGACTCCCCCGAATTCCACCGACAGTCCTGGGA GTTTTACCAGACCCTGTGTCAAGGAGAGTGGAAAGCCTCATTTGAAGAGCTCCACGATGTGGACCACTTT GAAATTGTTGAGAATCTGACCCAGAAGGACAACGTGCTCACCCAGATTATCTTGAAAACAATCTTCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217126 representing NM_001010982
Red=Cloning site Green=Tags(s) MMDVSGVGFPSKVPWKKMSAEELENQYCPSRWVVRLGAEEALRTYSQIGIEATTRARATRKSLLHVPYGD GEGEKVDIYFPDESSEALPFFLFFHGGYWQSGSKDESAFMVHPLTAQGVAVVIVAYGIAPKGTLDHMVDQ VTRSVAFVQKRYPSNKGIYLCGHSAGAHLAAMMLLADWTKHGVTPNLRGFFLVSGVFDLEPIVYTSQNVA LQLTLEDAQRNSPQLKVAQAQPVDPTCRVLVVVGQFDSPEFHRQSWEFYQTLCQGEWKASFEELHDVDHF EIVENLTQKDNVLTQIILKTIFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001010982 |
ORF Size | 909 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001010982.4 |
RefSeq Size | 1820 bp |
RefSeq ORF | 912 bp |
Locus ID | 125061 |
UniProt ID | Q63HM1 |
Cytogenetics | 17q25.3 |
Protein Pathways | Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Tryptophan metabolism |
MW | 33.8 kDa |
Gene Summary | Catalyzes the hydrolysis of N-formyl-L-kynurenine to L-kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217126L3 | Lenti ORF clone of Human arylformamidase (AFMID), transcript variant 2, Myc-DDK-tagged |
USD 986.00 |
|
RC217126L4 | Lenti ORF clone of Human arylformamidase (AFMID), transcript variant 2, mGFP tagged |
USD 986.00 |
|
RG217126 | AFMID (tGFP-tagged) - Human arylformamidase (AFMID), transcript variant 2 |
USD 886.00 |
|
SC301556 | AFMID (untagged)-Human arylformamidase (AFMID), transcript variant 2 |
USD 450.00 |
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