C18orf34 (CCDC178) (NM_198995) Human Tagged ORF Clone

CAT#: RC216329

CCDC178 (Myc-DDK-tagged)-Human chromosome 18 open reading frame 34 (C18orf34), transcript variant 2

Product Images

Circular map for RC216329

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  Western blot validation of overexpression lysate (Cat# LY404708) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216329 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CCDC178 protein (Cat# TP316329). The protein was produced from HEK293T cells transfected with CCDC178 cDNA clone (Cat# RC216329) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C18orf34
• Synonyms: C18orf34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216329 representing NM_198995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAAAACAAGACAGTTTCCTCTTCTTCCACTAGAGATGATCAAACCAATATAGGTTTAACATGTC
  AGGAAGTAAAGGCTCTCAGAGAGAAGGCATGGTCAAGGACAAATGAAGGCAATGCCATGTCTCAAAGTTT
  GGTTCTATATGGAGCCTCTAAGGAGAACAGTGAAGGTTTTCATGAAAGTAAAATGACAAATACTGAAGGG
  GTGAATAAAGGCATTTACTTTAGCTACCCATGTCGACGTCACAGCTGTGCCGTAGTAAATATTCCAGCAC
  CTTGTGTCAACAAAATGATTTCACACATCCAAGATGTGGAGTCCAAAATACAGGAGCATTTGAAAAGGTT
  TGAAACTTCTTTTGAAGAATGGAGCAGAACTTCTTCCACAAAAGACCTGAAAGAAGATTGGAGTGTAACT
  ACACCAGTGAAAGAGGTCAAACCAGGAGAAAAGAGAGATGAAAAGTGTCCAGAGTTAAAGCAGGAAATGG
  AAACATTGCTCTCAGAGGCCATTCGTCTCATTAAAAGTCTAGAAACTGACCGGGCAGACGCTGAAGAAGC
  TTTAAAACAACAGAGATCAAGAAAGAATATGATTAACATGAAAATTGACTCTTGGTCAGTCTGGAAACTT
  CAAGAACTCCCATTGGCTGTGCAGAAAGAACATGAGGCCTATTTGAGTGATGTTATAGAATTACAATGGC
  ATCTTGAAGATAAAGCTAATCAACTACAACATTTTGAAAAACAAAAGACAGAGTTAGAAGAAGCAAATGC
  AAAGATTCAAGCAGACATAGACTACATGAATGAACATGGCCCTCTACTGGACTCTAAGCAGAATCAGGAA
  CTTCAAGATCTGAAGAACCATTATAAAAAAAAAATGGAGGTAATGGACCTACACAGAAAAGTTAATGAAG
  AACTTGAAGAAGCTTTAGAAGCCTGTGAAAATGCCAGATTGAAGGCTCAGCAAATTAAAGAAGAGATTGA
  TAAGGATATTTACCAGGATGAAAAAACCATAGAGGCCTACAAGAGAGAGATATATCAACTTAACAGTCTA
  TTTGATCATTACTCTTCATCAGTGATAAATGTTAATACTAATATTGAGGAGAAGGAAGAGGAAGTGACTG
  AAGCAATAAGGGAAACAAAGTCATCAAAAAATGAATTACATTCTCTATCAAAAATGCTGGAAGATTTGAG
  AAGAGTTTATGACCAACTAACCTGGAAGCAAAAAAGTCATGAAAATCAGTATCTGGAAGCAGTTAATGAT
  TTTTATGCTGCAAAAAAAACATGGGATATTGAGCTTTCTGATGTTGCAAAAGATTTTTCAGCTATTTCTT
  TGGCATGTACAAAACTGACGGAAGACAATAAAAAACTTGAGATTGATATTAACAAAATAACAGTAAAAAC
  CAATGAAAGCATACGGAAAAAATCAAAATACGAATCTGAAATAAAATATTTGACAATAATGAAGTTAAAG
  AATGATAAACATCTCAAGAACATCTATAAGGAGGCTTATCGCATTGGTACTCTTTTCCACCTAACCAAAC
  ACAAGACAGATGAAATGGAAGATAAAATAGCAGAAGTGAGAAGAAAGTTCAAGGGTAGAGAAGAATTCCT
  GAAAAAACTCACTCAAGGTGAAGTGGCTGCTGGAATGGTGCTTCAGAAAAAACTATATTCCATTTACGAA
  GTCCAGGCACTTGAGCGGAAAGAGCTTATAAAAAATAGAGCAATATGTGCCATGTCACTGGCAGAACTAC
  AGGAACCTCTGCTTCAACTAGAAGATGAAGCTGAAAGAATCAGAAGTCTCGACAAAGAACATTCTGTTAG
  TAAACGCTCAGCAATTTTTAAAGACCTAGAAGCAACTAAAAGTAAGACAATGATTTTTTATGCAAAAATA
  AATGAATTGAATGAGGAATTAAAAGCAAAAGAAGAAGAAAAGAAAAGTTTTGATCAGACACTTGAAATAT
  TAAAGAACAAATTTATAACTATGAGATTTAAAAGGGAACATGCACAAACTGTGTTTGATCATTATATGCA
  AGAGAAAAAAGACTGTGAAGAGAGAATCTTTGAGGAAGATCAGAGATTTAGAGTGCTCCTTGCTGTAAGA
  CAAAAAACTCTTCAAGATACCCAAAAAATAATAGCTGATTCACTTGAAGAAAATCTGCGTTTAGCTCAAG
  AGTATCAACAGCTACAGATTACATTCTTAAAAGAAAAGGACAATTATTTCAATATATATGATAAACAGCT
  ATCACTTGATACTTCAATTAGAGATAAGAAACAGCTCTGTCAGCTGCAGAGAAGGATGCACACACTGTGG
  CAGGAGCACTTCAAACTGGTGGTCCTCTTCAGCCAGATGAGGCTGGCCAACTTCCAGACAGACTCTCAGG
  AGAGTATTCAGAAAATATTAGCTGTGCAGGAGGAATCTTCAAATTTAATGCAACACATCTTAGGTTTCTT
  CCAGACTTTGACAGATGGCACATGCGAAAACGATGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216329 representing NM_198995
  Red=Cloning site Green=Tags(s)
  
  MTENKTVSSSSTRDDQTNIGLTCQEVKALREKAWSRTNEGNAMSQSLVLYGASKENSEGFHESKMTNTEG
  VNKGIYFSYPCRRHSCAVVNIPAPCVNKMISHIQDVESKIQEHLKRFETSFEEWSRTSSTKDLKEDWSVT
  TPVKEVKPGEKRDEKCPELKQEMETLLSEAIRLIKSLETDRADAEEALKQQRSRKNMINMKIDSWSVWKL
  QELPLAVQKEHEAYLSDVIELQWHLEDKANQLQHFEKQKTELEEANAKIQADIDYMNEHGPLLDSKQNQE
  LQDLKNHYKKKMEVMDLHRKVNEELEEALEACENARLKAQQIKEEIDKDIYQDEKTIEAYKREIYQLNSL
  FDHYSSSVINVNTNIEEKEEEVTEAIRETKSSKNELHSLSKMLEDLRRVYDQLTWKQKSHENQYLEAVND
  FYAAKKTWDIELSDVAKDFSAISLACTKLTEDNKKLEIDINKITVKTNESIRKKSKYESEIKYLTIMKLK
  NDKHLKNIYKEAYRIGTLFHLTKHKTDEMEDKIAEVRRKFKGREEFLKKLTQGEVAAGMVLQKKLYSIYE
  VQALERKELIKNRAICAMSLAELQEPLLQLEDEAERIRSLDKEHSVSKRSAIFKDLEATKSKTMIFYAKI
  NELNEELKAKEEEKKSFDQTLEILKNKFITMRFKREHAQTVFDHYMQEKKDCEERIFEEDQRFRVLLAVR
  QKTLQDTQKIIADSLEENLRLAQEYQQLQITFLKEKDNYFNIYDKQLSLDTSIRDKKQLCQLQRRMHTLW
  QEHFKLVVLFSQMRLANFQTDSQESIQKILAVQEESSNLMQHILGFFQTLTDGTCENDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198995
• ORF Size: 2487 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198995.3
• RefSeq Size: 3087 bp
• RefSeq ORF: 2490 bp
• Locus ID: 374864
• UniProt ID: Q5BJE1
• Cytogenetics: 18q12.1
• MW: 97.4 kDa