AKAP6 (NM_004274) Human Tagged ORF Clone

CAT#: RC215986

AKAP6 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 6 (AKAP6)

Product Images

Circular map for RC215986

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKAP6
• Synonyms: ADAP6; ADAP100; AKAP100; mAKAP; PRKA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215986 representing NM_004274.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTAACCATGAGCGTGACACTTTCCCCCCTGAGGTCACAGGACCTGGATCCCATGGCTACTGATGCT
  TCACCCATGGCCATCAACATGACACCCACTGTGGAGCAGGGTGAGGGAGAAGAGGCAATGAAGGACATG
  GACTCTGACCAGCAGTATGAAAAGCCACCCCCACTACACACAGGGGCTGACTGGAAGATTGTCCTCCAC
  TTACCTGAAATTGAGACCTGGCTCCGGATGACCTCAGAGAGGGTCCGAGACCTAACCTATTCAGTCCAG
  CAGGATTCGGACAGCAAGCATGTGGATGTACATCTAGTTCAACTAAAGGACATTTGTGAAGATATTTCT
  GATCATGTTGAGCAAATCCATGCCCTCCTTGAAACAGAGTTCTCCCTAAAGCTGCTGTCTTACTCTGTC
  AACGTGATAGTGGACATCCACGCAGTGCAGCTCCTCTGGCACCAGCTTCGAGTCTCAGTGCTGGTTCTG
  CGGGAGCGCATTCTGCAAGGTCTGCAGGACGCCAATGGCAACTACACTAGGCAGACGGACATTCTGCAA
  GCTTTCTCTGAAGAGACAAAAGAGGGCCGGCTTGATTCTCTAACAGAAGTGGATGACTCAGGACAATTA
  ACCATCAAATGTTCTCAAAATTACTTGTCTCTGGATTGTGGCATTACTGCATTCGAACTGTCTGACTAC
  AGTCCAAGTGAGGATTTGCTCAGTGGGCTAGGTGACATGACCTCTAGCCAAGTCAAAACCAAACCCTTT
  GACTCTTGGAGCTACAGTGAGATGGAAAAGGAGTTTCCTGAGCTTATCCGAAGTGTTGGTTTACTTACG
  GTAGCTGCTGACTCTATCTCTACCAATGGCAGTGAAGCAGTTACTGAGGAGGTATCTCAAGTATCTCTC
  TCAGTAGACGACAAAGGTGGATGTGAGGAAGACAATGCTTCTGCAGTCGAAGAGCAACCAGGCTTAACA
  CTGGGGGTGTCATCATCTTCAGGAGAAGCTCTGACAAATGCTGCTCAACCCTCCTCTGAGACTGTGCAG
  CAAGAATCCAGTTCCTCCTCCCATCATGATGCAAAGAATCAGCAGCCTGTTCCTTGTGAAAATGCAACC
  CCCAAACGAACCATCAGAGATTGCTTTAATTATAACGAGGACTCTCCCACACAGCCTACATTGCCAAAA
  AGAGGACTTTTTCTTAAAGAGGAAACTTTTAAGAATGATCTGAAAGGCAATGGTGGAAAGAGGCAAATG
  GTTGATCTAAAGCCTGAGATGAGCAGAAGCACCCCTTCGCTAGTAGATCCTCCTGACAGATCCAAACTT
  TGCCTGGTATTGCAGTCTTCTTACCCCAACAGCCCTTCTGCTGCCAGCCAGTCTTATGAGTGTTTACAC
  AAGGTGGGGAATGGGAACCTTGAAAACACAGTCAAATTTCACATTAAAGAAATTTCTTCCAGCCTGGGA
  AGGCTTAACGACTGCTATAAAGAGAAATCTCGACTTAAAAAGCCACACAAGACCTCAGAAGAGGTGCCT
  CCATGCCGAACACCTAAACGGGGGACTGGTTCAGGCAAACAAGCTAAAAATACAAAGAGCTCAGCAGTG
  CCAAATGGAGAGCTTTCTTATACTTCCAAGGCCATAGAGGGGCCACAAACAAATTCTGCTTCCACATCC
  TCACTTGAGCCTTGTAATCAGAGAAGTTGGAATGCCAAATTGCAATTGCAGTCAGAAACATCCAGTTCA
  CCAGCTTTTACTCAGAGCAGTGAATCCTCTGTTGGCTCAGACAACATCATGTCTCCGGTGCCACTTCTT
  TCAAAACACAAAAGCAAAAAAGGTCAAGCCTCCTCTCCAAGTCACGTCACTAGGAATGGTGAGGTTGTG
  GAGGCCTGGTATGGCTCTGATGAATACCTAGCACTGCCCTCTCACCTTAAGCAGACAGAAGTATTGGCT
  TTGAAGTTGGAAAACCTAACAAAGCTTCTGCCTCAGAAACCCAGAGGAGAAACCATCCAGAATATTGAT
  GACTGGGAACTGTCTGAAATGAATTCAGATTCTGAAATCTATCCAACCTATCATGTCAAAAAGAAGCAT
  ACAAGGCTAGGCAGGGTGTCTCCAAGCTCATCTAGTGACATAGCCTCTTCACTAGGGGAGAGCATTGAA
  TCTGGGCCCCTGAGTGACATTCTTTCTGATGAGGAGTCCAGTATGCCTCTCGCTGGCATGAAAAAGTAT
  GCTGATGAGAAGTCAGAAAGAGCTTCATCCTCTGAGAAAAATGAGAGCCATTCTGCCACTAAATCAGCT
  TTAATTCAGAAACTGATGCAAGATATTCAGCACCAAGACAACTATGAAGCCATATGGGAAAAAATAGAG
  GGGTTTGTAAACAAACTGGATGAATTCATTCAATGGTTAAATGAAGCCATGGAAACTACAGAGAATTGG
  ACTCCCCCTAAAGCAGAGATGGATGACCTTAAACTGTATCTGGAGACACACTTGAGTTTTAAGTTGAAT
  GTAGACAGTCATTGTGCTCTCAAGGAAGCTGTGGAGGAGGAAGGACACCAACTTCTTGAGCTTATTGCA
  TCTCACAAAGCAGGACTGAAGGACATGCTGCGGATGATTGCAAGTCAATGGAAGGAGCTGCAGAGGCAA
  ATCAAACGGCAGCACAGCTGGATTCTCAGGGCTCTGGATACCATCAAAGCCGAGATACTGGCTACTGAT
  GTGTCTGTGGAGGATGAGGAAGGGACTGGAAGCCCCAAGGCTGAGGTTCAACTATGCTACCTGGAAGCA
  CAAAGAGATGCTGTTGAGCAGATGTCCCTCAAGCTGTACAGCGAGCAGTATACCAGCAGCAGCAAGCGA
  AAGGAAGAGTTTGCTGATATGTCAAAAGTTCATTCAGTGGGAAGCAATGGGCTTCTGGACTTTGATTCA
  GAATATCAGGAGCTCTGGGATTGGCTGATTGACATGGAGTCCCTTGTGATGGACAGCCACGACCTGATG
  ATGTCAGAGGAGCAGCAGCAGCATCTTTACAAGCGATACAGTGTGGAAATGTCCATCAGACACCTGAAA
  AAGACGGAGCTGCTTAGTAAGGTTGAAGCTTTGAAGAAAGGTGGCGTTTTACTACCAAATGATCTCCTT
  GAAAAAGTGGATTCAATTAATGAAAAATGGGAACTGCTTGGGAAAACCCTAGGAGAGAAGATCCAGGAC
  ACAATGGCAGGGCACAGTGGGTCGAGTCCACGTGACCTGCTCTCTCCTGAAAGTGGAAGCCTGGTAAGG
  CAGCTGGAGGTCAGGATCAAAGAACTGAAAGGATGGCTAAGAGATACAGAGCTTTTCATCTTCAATTCC
  TGTCTGAGACAAGAAAAGGAAGGAACAATGAATACTGAGAAACAACTGCAATACTTTAAGTCCCTCTGT
  CGTGAAATCAAGCAACGACGTCGAGGAGTTGCCTCCATTCTGCGACTATGCCAGCATCTTTTGGATGAC
  CGGGAGACTTGCAATCTGAATGCAGATCACCAGCCCATGCAGCTGATCATTGTAAATCTTGAAAGAAGG
  TGGGAAGCCATTGTCATGCAAGCCGTCCAGTGGCAAACACGTCTACAAAAGAAGATGGGAAAGGAATCT
  GAGACTTTGAATGTGATTGATCCTGGCTTGATGGACCTAAATGGGATGAGTGAGGATGCCCTGGAATGG
  GATGAAATGGACATAAGTAACAAGTTAATTAGTTTGAATGAGGAATCAAATGACCTTGATCAAGAACTC
  CAACCTGTTATCCCTTCCTTGAAGCTTGGAGAGACAAGTAATGAGGACCCTGGTTATGACGAGGAGGCT
  GATAACCATGGGGGATCTCAGTATGCCTCAAATATTACTGCCCCCTCTAGTCCACACATTTACCAGGTG
  TACAGCCTCCACAATGTTGAACTCTATGAGGACAACCACATGCCATTTCTGAAAAACAATCCAAAGGTC
  ACTGGCATGACACAGCCTAATGTTTTAACTAAGAGTCTCAGTAAAGACTCTTCATTTTCATCTACCAAA
  TCTTTGCCAGATCTTCTAGGTGGTTCCAATTTGGTAAAGCCCTGCGCATGTCATGGAGGAGACATGAGC
  CAGAATTCAGGCAGTGAGAGTGGAATTGTCAGTGAAGGAGACACAGAAACCACTACCAACTCTGAAATG
  TGCTTGCTCAATGCAGTGGATGGGTCCCCAAGTAACCTTGAAACTGAACATCTGGACCCACAAATGGGA
  GATGCAGTTAACGTGTTAAAGCAAAAATTTACAGATGAGGGGGAAAGCATTAAGCTTCCAAATAGCTCT
  CAGTCGTCCATTTCACCAGTGGGTTGTGTAAATGGAAAAGTTGGAGATTTAAACAGTATTACCAAACAT
  ACCCCTGACTGTTTGGGAGAAGAATTACAAGGAAAACATGATGTGTTTACATTTTATGATTACTCATAC
  CTCCAAGGCTCAAAACTCAAATTACCAATGATAATGAAACAGTCACAAAGCGAAAAAGCGCATGTGGAG
  GATCCCCTGCTTCGTGGTTTTTATTTTGATAAAAAATCATGCAAATCTAAACATCAGACTACAGAGTTA
  CAACCAGATGTACCTCCCCATGAAAGGATTTTGGCAAGTGCATCTCATGAAATGGATCGCATTTCATAT
  AAAAGTGGCAATATAGAAAAGACATTCACTGGCATGCAGAATGCCAAACAGCTCTCCCTTTTATCTCAT
  AGTTCATCTATTGAGTCCCTTTCTCCAGGGGGTGATTTATTTGGATTGGGCATCTTTAAAAATGGCAGT
  GACAGCCTCCAGCGAAGCACTTCTTTAGAAAGTTGGTTGACTTCCTATAAAAGCAATGAAGATCTCTTT
  AGCTGTCACAGCTCTGGGGATATAAGCGTGAGCAGTGGCTCAGTTGGTGAACTAAGTAAAAGAACATTA
  GATCTCCTGAATCGTTTGGAGAATATCCAGAGCCCCTCAGAGCAAAAGATAAAACGAAGTGTTTCTGAT
  ATCACTCTTCAAAGCAGTTCCCAAAAGATGTCCTTTACTGGCCAGATGTCATTGGACATAGCATCTTCT
  ATCAATGAAGACTCAGCGGCATCTCTAACAGAACTTAGCAGCAGTGACGAGCTCTCTCTTTGCTCAGAG
  GATATTGTGTTACACAAGAACAAGATCCCGGAATCGAATGCATCGTTCAGGAAGCGTCTGACTCGTTCA
  GTGGCTGATGAAAGCGATGTCAATGTCAGCATGATTGTTAATGTCTCTTGCACCTCTGCTTGCACTGAT
  GATGAAGATGACAGCGACCTGCTCTCCAGCTCTACCCTTACCTTGACTGAAGAAGAGCTGTGCATCAAA
  GATGAGGATGACGACTCCAGTATTGCAACAGATGATGAAATTTATGAAGACTGCACCTTGATGTCAGGG
  CTAGACTACATAAAGAATGAATTACAGACCTGGATTAGGCCAAAATTGTCTTTGACAAGAGATAAGAAA
  AGGTGCAATGTCAGTGATGAGATGAAGGGCAGTAAAGATATAAGTAGCAGTGAGATGACCAATCCCTCT
  GATACTCTGAATATTGAGACCCTTCTAAATGGCTCTGTAAAACGTGTCTCTGAAAATAATGGAAATGGT
  AAGAATTCATCTCATACCCATGAGTTAGGGACAAAGCGTGAAAATAAGAAAACTATTTTCAAAGTTAAT
  AAAGATCCATATGTGGCTGACATGGAAAATGGCAATATTGAAGGTATTCCAGAAAGGCAAAAGGGCAAA
  CCGAATGTGACTTCAAAGGTATCAGAAAATCTTGGTTCACATGGGAAAGAGATTTCAGAGAGTGAGCAT
  TGTAAGTGTAAAGCACTTATGGATAGTTTAGATGATTCAAATACTGCTGGCAAGGAATTTGTTTCCCAA
  GATGTTAGACATCTTCCAAAGAAATGTCCAAATCACCACCATTTTGAAAATCAAAGCACTGCCTCTACT
  CCCACTGAGAAGTCTTTCTCAGAACTGGCTTTAGAAACCAGGTTTAACAACAGACAAGACTCTGATGCA
  CTGAAATCATCTGATGATGCACCGAGTATGGCTGGAAAATCTGCTGGTTGTTGCCTAGCACTTGAACAA
  AACGGAACAGAGGAAAATGCTTCTATCAGCGACATTTCCTGTTGCAACTGTGAGCCAGATGTTTTCCAT
  CAAAAAGATGCCGAAGATTGTTCAGTACACAACTTTGTTAAGGAAATCATTGACATGGCTTCGACAGCC
  CTAAAAAGTAAATCTCAACCTGAAAACGAGGTGGCTGCTCCTACTTCATTAACTCAAATCAAGGAGAAA
  GTGTTGGAGCATTCTCACCGGCCCATCCAGCTGAGAAAAGGGGACTTTTATTCGTACTTATCTCTCTCA
  TCTCATGACAGTGATTGTGGGGAGGTCACCAATTACATAGAAGAGAAAAGCAGCACTCCATTGCCACTA
  GACACCACTGACTCGGGCTTAGATGACAAGGAAGATATTGAATGCTTTTTTGAGGCCTGTGTTGAGGGT
  GACTCTGATGGAGAGGAGCCTTGTTACTCTAGTGCTCCTCCAAATGAATCTGCAGTTCCCAGCGAAGCT
  GCAATGCCACTACAAGCAACAGCATGTTCTTCTGAGTTCAGTGATAGTTCTCTTTCAGCTGATGATGCA
  GATACAGTGGCTCTTTCAAGTCCTTCCTCTCAGGAAAGAGCTGAGGTTGGAAAGGAAGTGAATGGTTTG
  CCCCAAACTTCCAGTGGCTGTGCAGAAAACTTAGAGTTTACTCCTTCAAAGCTTGACAGTGAAAAGGAA
  AGTTCCGGAAAACCAGGTGAATCTGGAATGCCAGAAGAACATAATGCTGCTTCAGCCAAATCTAAAGTT
  CAAGACCTCTCCTTGAAGGCAAATCAGCCAACAGACAAGGCCGCATTGCATCCCAGCCCCAAAACTTTA
  ACCTGTGAAGAAAATCTTCTAAACCTTCATGAAAAACGACATAGAAATATGCATAGG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC215986
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLTMSVTLSPLRSQDLDPMATDASPMAINMTPTVEQGEGEEAMKDMDSDQQYEKPPPLHTGADWKIVLH
  LPEIETWLRMTSERVRDLTYSVQQDSDSKHVDVHLVQLKDICEDISDHVEQIHALLETEFSLKLLSYSV
  NVIVDIHAVQLLWHQLRVSVLVLRERILQGLQDANGNYTRQTDILQAFSEETKEGRLDSLTEVDDSGQL
  TIKCSQNYLSLDCGITAFELSDYSPSEDLLSGLGDMTSSQVKTKPFDSWSYSEMEKEFPELIRSVGLLT
  VAADSISTNGSEAVTEEVSQVSLSVDDKGGCEEDNASAVEEQPGLTLGVSSSSGEALTNAAQPSSETVQ
  QESSSSSHHDAKNQQPVPCENATPKRTIRDCFNYNEDSPTQPTLPKRGLFLKEETFKNDLKGNGGKRQM
  VDLKPEMSRSTPSLVDPPDRSKLCLVLQSSYPNSPSAASQSYECLHKVGNGNLENTVKFHIKEISSSLG
  RLNDCYKEKSRLKKPHKTSEEVPPCRTPKRGTGSGKQAKNTKSSAVPNGELSYTSKAIEGPQTNSASTS
  SLEPCNQRSWNAKLQLQSETSSSPAFTQSSESSVGSDNIMSPVPLLSKHKSKKGQASSPSHVTRNGEVV
  EAWYGSDEYLALPSHLKQTEVLALKLENLTKLLPQKPRGETIQNIDDWELSEMNSDSEIYPTYHVKKKH
  TRLGRVSPSSSSDIASSLGESIESGPLSDILSDEESSMPLAGMKKYADEKSERASSSEKNESHSATKSA
  LIQKLMQDIQHQDNYEAIWEKIEGFVNKLDEFIQWLNEAMETTENWTPPKAEMDDLKLYLETHLSFKLN
  VDSHCALKEAVEEEGHQLLELIASHKAGLKDMLRMIASQWKELQRQIKRQHSWILRALDTIKAEILATD
  VSVEDEEGTGSPKAEVQLCYLEAQRDAVEQMSLKLYSEQYTSSSKRKEEFADMSKVHSVGSNGLLDFDS
  EYQELWDWLIDMESLVMDSHDLMMSEEQQQHLYKRYSVEMSIRHLKKTELLSKVEALKKGGVLLPNDLL
  EKVDSINEKWELLGKTLGEKIQDTMAGHSGSSPRDLLSPESGSLVRQLEVRIKELKGWLRDTELFIFNS
  CLRQEKEGTMNTEKQLQYFKSLCREIKQRRRGVASILRLCQHLLDDRETCNLNADHQPMQLIIVNLERR
  WEAIVMQAVQWQTRLQKKMGKESETLNVIDPGLMDLNGMSEDALEWDEMDISNKLISLNEESNDLDQEL
  QPVIPSLKLGETSNEDPGYDEEADNHGGSQYASNITAPSSPHIYQVYSLHNVELYEDNHMPFLKNNPKV
  TGMTQPNVLTKSLSKDSSFSSTKSLPDLLGGSNLVKPCACHGGDMSQNSGSESGIVSEGDTETTTNSEM
  CLLNAVDGSPSNLETEHLDPQMGDAVNVLKQKFTDEGESIKLPNSSQSSISPVGCVNGKVGDLNSITKH
  TPDCLGEELQGKHDVFTFYDYSYLQGSKLKLPMIMKQSQSEKAHVEDPLLRGFYFDKKSCKSKHQTTEL
  QPDVPPHERILASASHEMDRISYKSGNIEKTFTGMQNAKQLSLLSHSSSIESLSPGGDLFGLGIFKNGS
  DSLQRSTSLESWLTSYKSNEDLFSCHSSGDISVSSGSVGELSKRTLDLLNRLENIQSPSEQKIKRSVSD
  ITLQSSSQKMSFTGQMSLDIASSINEDSAASLTELSSSDELSLCSEDIVLHKNKIPESNASFRKRLTRS
  VADESDVNVSMIVNVSCTSACTDDEDDSDLLSSSTLTLTEEELCIKDEDDDSSIATDDEIYEDCTLMSG
  LDYIKNELQTWIRPKLSLTRDKKRCNVSDEMKGSKDISSSEMTNPSDTLNIETLLNGSVKRVSENNGNG
  KNSSHTHELGTKRENKKTIFKVNKDPYVADMENGNIEGIPERQKGKPNVTSKVSENLGSHGKEISESEH
  CKCKALMDSLDDSNTAGKEFVSQDVRHLPKKCPNHHHFENQSTASTPTEKSFSELALETRFNNRQDSDA
  LKSSDDAPSMAGKSAGCCLALEQNGTEENASISDISCCNCEPDVFHQKDAEDCSVHNFVKEIIDMASTA
  LKSKSQPENEVAAPTSLTQIKEKVLEHSHRPIQLRKGDFYSYLSLSSHDSDCGEVTNYIEEKSSTPLPL
  DTTDSGLDDKEDIECFFEACVEGDSDGEEPCYSSAPPNESAVPSEAAMPLQATACSSEFSDSSLSADDA
  DTVALSSPSSQERAEVGKEVNGLPQTSSGCAENLEFTPSKLDSEKESSGKPGESGMPEEHNAASAKSKV
  QDLSLKANQPTDKAALHPSPKTLTCEENLLNLHEKRHRNMHR
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004274
• ORF Size: 6957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 10342 bp
• RefSeq ORF: 6960 bp
• Locus ID: 9472
• UniProt ID: Q13023
• Cytogenetics: 14q12
• Domains: spectrin
• Protein Families: Druggable Genome
• MW: 256.7 kDa
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum. [provided by RefSeq, Jul 2008]