FTO (NM_001080432) Human Tagged Lenti ORF Clone
CAT#: RC215889L2
- LentiORF®
Lenti ORF clone of Human fat mass and obesity associated (FTO), mGFP tagged
"NM_001080432" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | FTO |
Synonyms | ALKBH9; BMIQ14; GDFD |
Vector | pLenti-C-mGFP |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC215889).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001080432 |
ORF Size | 1515 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001080432.1 |
RefSeq Size | 4294 bp |
RefSeq ORF | 1518 bp |
Locus ID | 79068 |
UniProt ID | Q9C0B1 |
Cytogenetics | 16q12.2 |
MW | 58.1 kDa |
Gene Summary | This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215889 | FTO (Myc-DDK-tagged)-Human fat mass and obesity associated (FTO) |
USD 470.00 |
|
RC215889L1 | Lenti ORF clone of Human fat mass and obesity associated (FTO), Myc-DDK-tagged |
USD 770.00 |
|
RC215889L3 | Lenti ORF clone of Human fat mass and obesity associated (FTO), Myc-DDK-tagged |
USD 770.00 |
|
RC215889L4 | Lenti ORF clone of Human fat mass and obesity associated (FTO), mGFP tagged |
USD 770.00 |
|
RG215889 | FTO (tGFP-tagged) - Human fat mass and obesity associated (FTO) |
USD 670.00 |
|
SC315786 | FTO (untagged)-Human fat mass and obesity associated (FTO) |
USD 471.00 |
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