FTO (NM_001080432) Human Untagged Clone

CAT#: SC315786

FTO (untagged)-Human fat mass and obesity associated (FTO)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FTO
• Synonyms: ALKBH9; BMIQ14; GDFD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001080432 edited
  GGCAGCATGAAGCGCACCCCGACTGCCGAGGAACGAGAGCGCGAAGCTAAGAAACTGAGG
  CTTCTTGAAGAGCTTGAAGACACTTGGCTCCCTTATCTGACCCCCAAAGATGATGAATTC
  TATCAGCAGTGGCAGCTGAAATATCCTAAACTAATTCTCCGAGAAGCCAGCAGTGTATCT
  GAGGAGCTCCATAAAGAGGTTCAAGAAGCCTTTCTCACACTGCACAAGCATGGCTGCTTA
  TTTCGGGACCTGGTTAGGATCCAAGGCAAAGATCTGCTCACTCCGGTATCTCGCATCCTC
  ATTGGTAATCCAGGCTGCACCTACAAGTACCTGAACACCAGGCTCTTTACGGTCCCCTGG
  CCAGTGAAAGGGTCTAATATAAAACACACCGAGGCTGAAATAGCCGCTGCTTGTGAGACC
  TTCCTCAAGCTCAATGACTACCTGCAGATAGAAACCATCCAGGCTTTGGAAGAACTTGCT
  GCCAAAGAGAAGGCTAATGAGGATGCTGTGCCATTGTGTATGTCTGCAGATTTCCCCAGG
  GTTGGGATGGGTTCATCCTACAACGGACAAGATGAAGTGGACATTAAGAGCAGAGCAGCA
  TACAACGTAACTTTGCTGAATTTCATGGATCCTCAGAAAATGCCATACCTGAAAGAGGAA
  CCTTATTTTGGCATGGGGAAAATGGCAGTGAGCTGGCATCATGATGAAAATCTGGTGGAC
  AGGTCAGCGGTGGCAGTGTACAGTTATAGCTGTGAAGGCCCTGAAGAGGAAAGTGAGGAT
  GACTCTCATCTCGAAGGCAGGGATCCTGATATTTGGCATGTTGGTTTTAAGATCTCATGG
  GACATAGAGACACCTGGTTTGGCGATACCCCTTCACCAAGGAGACTGCTATTTCATGCTT
  GATGATCTCAATGCCACCCACCAACACTGTGTTTTGGCCGGTTCACAACCTCGGTTTAGT
  TCCACCCACCGAGTGGCAGAGTGCTCAACAGGAACCTTGGATTATATTTTACAACGCTGT
  CAGTTGGCTCTGCAGAATGTCTGTGACGATGTGGACAATGATGATGTCTCTTTGAAATCC
  TTTGAGCCTGCAGTTTTGAAACAAGGAGAAGAAATTCATAATGAGGTCGAGTTTGAGTGG
  CTGAGGCAGTTTTGGTTTCAAGGCAATCGATACAGAAAGTGCACTGACTGGTGGTGTCAA
  CCCATGGCTCAACTGGAAGCACTGTGGAAGAAGATGGAGGGTGTGACAAATGCTGTGCTT
  CATGAAGTTAAAAGAGAGGGGCTCCCCGTGGAACAAAGGAATGAAATCTTGACTGCCATC
  CTTGCCTCGCTCACTGCACGCCAGAACCTGAGGAGAGAATGGCATGCCAGGTGCCAGTCA
  CGAATTGCCCGAACATTACCTGCTGATCAGAAGCCAGAATGTCGGCCATACTGGGAAAAG
  GATGATGCTTCGATGCCTCTGCCGTTTGACCTCACAGACATCGTTTCAGAACTCAGAGGT
  CAGCTTCTGGAAGCAAAACCCTAGAAGGAGCACAAGTCTCAGGCGGAGGAGAAAAAGAGA
  TCGGCTTTTCTCCTCCAACGTTGTCATGGGCTTAAGCAAGAGCAGTGGAGACTTCTCTTG
  GCCCCTAGATTGTAGCACCCGGGTCCCAATCCAAAACAGCTAGGAAATGGTGCCCATGAA
  GTTTTAAATGTTTTAAAATGACCCTGTGTTATAGTCTGATTTGGTGTTAAACAGGACCTT
  CTTCCCCCAAAATTGTTCAGATTATAAAATGTGAGCCATTCAGCCCCCAAGGTCCAGGGC
  AGGCGACAGGAACGAGCCCAGCGTGTGACAAAGCCTAACCTACTTTCCTCTTTCCCAAGC
  TTTTTCAGAGACTCTGGAGTGGACCCAGCCCTCTGGGGAAAGACAGAACTTAGAGACATC
  CCAGTTACTCACCACACCCATAGTGCTGTCCAATATGGTAGCCACTAGCTAGCTGTGGCT
  ACTTCAATTTAAATTCAGTTTTAATTTTAATTAAAAATGCAGCTCTTCAGTCGCCCTGGC
  CACATTTCAAGTGCTTAACAGCCTCATGTGGCTAGTGACTGCTGTATTGGACGGTACAGA
  TATGGAACATTTTCATCATCGAAGAAAGTCCTATTGGACAACACTTCTATAAAAAGTTTG
  AGAGCAGGAATTCTCATTTCCATTCGTCTGTAGCTTCTATCCCCAAAGGCAAAGAAACTA
  AAAGAGAAATGACTCATTGAAGATTGGCCTCTTTCCTTTCTCTAAGACAAACCTAAGTAA
  AAGCCTGAGCTTTGAGTCCTATGCTCAGCACACGGGAAGGAGATGTTAATAATTAAAATA
  AAGTTGATATCCTGTCTTTAGGGAGTTCCCTTGATCTCTTGAAAGAGACACAGCCCCATT
  TACATTATTTCGTGGATTTCACCAGCATAGTATAGTTTTTTTCTGTAAGTCCCTCATTCT
  TATGTAATAACAGGTGGAACTGAGGTTTGAAGAACCTCAGTGGCCCATCCTGATGACATT
  GGAGACTCAAAGAGACAAGAGAGAGTAGGGTTTAAAACCTGAGCTTTAAGACTCCCACTA
  GCTTCGTGTCCTTTGGCATGTTAACGTGCCTCAGTTTCCTCATCTGTATAATGGGGATAT
  ATGAAAGGCACCAGTCCTAAGGTGAACATTAAGTGAGATGATTCTAGTTACAGACTTAGA
  ACAATTTCCAGCACATAGTTAAATATCCAGGAAATTCTGGTACTGTTATGTGTGGGTGAG
  CTGACCTGGATGTAGATGTTTTCCTCTCTCTTGCTGACCCCTCCGCCAGTTTTGTCTTGT
  GATGCCATTAACACATCTCTCCCTTTCTGACCTGGCTCCTGCCCATTGGTGTCCCAAGAA
  ATCGTGAGAATAGTTAGCCCCCCGTCTCCCCAGCCTGTTGCTTTCTCGTGTAGTTGTTCA
  CAGTAGTTGAGAAGTTGAAGAGCTTTTGCCTATTGAAGGTGCACTGAGAATAAACTCTTT
  CCTGCCACCAGAATTGCAGTGGTTCACGGCCTGCACTCATTCCCATGAATGCAGTTAATA
  GCCACAGAAATGTCACATTAAGCAAAGCAGCCAGGGTCTCATCGTGTTGAGACTCGAGTC
  TCTCAGACCTTGGATTCATTCCCTGGTGTCTTTGAGCCTCAGTTTCCTCATTGGTAAAAG
  AGAAGTGAAGCAGTGTCTCACAGGGTCATTACAGAGATTAAATGAAATAAATGAAATAAC
  ATAGACCAGGAGGGCGTGGTGTTTAAAAGTCACAGATGGGGCACCCTCGGGCCATCCAGC
  CCAGTGTTTTCTTTAGCCCCTATGATGTTCATTTTTTGTTATATCCCATTAGGTGCCCAT
  ATTTAAAAATTGGGAGATTTCACATAAAATTAAAAGGTCTGCATTTTCTTTTTTCTTTTC
  TTTTTTTTTTTTTTTGAGACACAGTCTCACTCTGTCACCAGGCTAGAGTGCAGTGGCACG
  ATCTCAGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGTAATTCTCCTGCCTCAGCCTCC
  CAAGTAGCTGGGACTACAGGCACGTGCCACCACGCCCAGCTAATTTTTGTATTTTTAGCA
  GAGATGGGGTTTCACCACATTGGCCAGGATGATCTCGATCTCAACCTCGTGATCCACCCA
  CCTCGGTCTCCCAAAGCGCTGGGATTACAGGCGTGAGCCACCGCGCCAAGCCAAGGTCTG
  CATTTTTCTTTAGAACTCAGAACACCCAATAGTCCTAGGCCCCCATCCTCGCATGGCAGC
  AAGCTAAATAAGCATCTTCCCACTGCGAGTTGGGGCATGACCCAGCCTATGGTTTGCCAT
  ACTCCCTCTTTTTCTCCGTTTTTTCATTAATTGTGAACCTGACCTGCATCACCCTTTCAT
  GTCAGTGCTCTCCAAACCTGCTTGCTTGCACCCCTCTAGTCGAAATATTTTGTGCTTACC
  CCAATATATGTGTGTGACTATTGAACTCTATTCGTAGACTGCTTGTACTAATGTCATTTG
  CATCATAAAATATTCATATCCAATAAACATATTAAAAGGATGAGATAAGAAAAAAAAAAA
  AAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001080432
• Insert Size: 4100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080432.1, NP_001073901.1
• RefSeq Size: 4294 bp
• RefSeq ORF: 1518 bp
• Locus ID: 79068
• UniProt ID: Q9C0B1
• Cytogenetics: 16q12.2
• Gene Summary: This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes. [provided by RefSeq, Jul 2011]