ZDHHC20 (NM_153251) Human Tagged ORF Clone

CAT#: RC215317

ZDHHC20 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 20 (ZDHHC20)

Product Images

Western blot validation of overexpression lysate (Cat# LY407166) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215317 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZDHHC20
• Synonyms: 4933421L13Rik; DHHC-20; DHHC20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215317 representing NM_153251
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCTGGACGCTGTGGCGCTGCTGCCAGCGCGTCGTGGGCTGGGTGCCGGTGCTCTTCATCACCT
  TCGTGGTCGTCTGGTCCTACTACGCGTACGTGGTGGAGCTCTGCGTGTTTACTATTTTTGGAAATGAAGA
  AAATGGAAAGACCGTTGTTTACCTTGTGGCTTTCCATCTGTTCTTTGTTATGTTTGTATGGTCCTATTGG
  ATGACAATTTTCACATCTCCCGCTTCCCCCTCCAAAGAGTTCTACTTGTCCAATTCTGAAAAGGAACGTT
  ATGAAAAAGAATTCAGCCAAGAAAGACAACAAGAAATTTTGAGAAGAGCAGCAAGAGCTTTACCTATCTA
  TACCACATCAGCTTCAAAAACTATCAGATATTGTGAAAAATGTCAGCTGATTAAACCTGATCGGGCGCAT
  CACTGCTCAGCCTGTGACTCATGTATTCTTAAGATGGATCATCACTGTCCTTGGGTGAATAACTGTGTGG
  GATTTTCTAATTACAAATTCTTCCTGCTGTTTTTATTGTATTCCCTATTATATTGCCTTTTCGTGGCTGC
  AACAGTTTTAGAGTACTTTATAAAATTTTGGACGAATGAACTGACAGATACACGTGCAAAATTCCACGTA
  CTTTTTCTTTTCTTTGTGTCTGCAATGTTCTTCATCAGCGTCCTCTCACTTTTCAGCTACCACTGCTGGC
  TAGTTGGAAAAAATAGAACAACAATAGAATCATTCCGCGCACCCACGTTTTCATACGGACCTGATGGAAA
  TGGTTTCTCTCTTGGATGCAGTAAAAATTGGAGACAAGTCTTTGGTGATGAAAAGAAATATTGGCTACTT
  CCAATATTTTCAAGCTTGGGTGATGGTTGCAGTTTTCCAACTCGCCTTGTGGGGATGGATCCAGAACAAG
  CTTCTGTTACAAACCAGAATGAGTATGCCAGAAGTGGCTCAAATCAACCTTTTCCTATCAAACCACTTAG
  TGAATCAAAAAACCGCTTGTTGGACAGTGAATCTCAGTGGCTGGAGAATGGAGCTGAAGAAGGCATCGTC
  AAATCAGGTGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215317 representing NM_153251
  Red=Cloning site Green=Tags(s)
  
  MAPWTLWRCCQRVVGWVPVLFITFVVVWSYYAYVVELCVFTIFGNEENGKTVVYLVAFHLFFVMFVWSYW
  MTIFTSPASPSKEFYLSNSEKERYEKEFSQERQQEILRRAARALPIYTTSASKTIRYCEKCQLIKPDRAH
  HCSACDSCILKMDHHCPWVNNCVGFSNYKFFLLFLLYSLLYCLFVAATVLEYFIKFWTNELTDTRAKFHV
  LFLFFVSAMFFISVLSLFSYHCWLVGKNRTTIESFRAPTFSYGPDGNGFSLGCSKNWRQVFGDEKKYWLL
  PIFSSLGDGCSFPTRLVGMDPEQASVTNQNEYARSGSNQPFPIKPLSESKNRLLDSESQWLENGAEEGIV
  KSGV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153251
• ORF Size: 1062 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153251.3, NP_694983.2
• RefSeq Size: 1514 bp
• RefSeq ORF: 1065 bp
• Locus ID: 253832
• UniProt ID: Q5W0Z9
• Cytogenetics: 13q12.11
• Protein Families: Transmembrane
• MW: 40.9 kDa
• Gene Summary: Catalyzes palmitoylation of Cys residues on target proteins (PubMed:27153536, PubMed:29326245). Catalyzes palmitoylation of Cys residues in the cytoplasmic C-terminus of EGFR, and modulates the duration of EGFR signaling by modulating palmitoylation-dependent EGFR internalization and degradation (PubMed:27153536). Has a preference for acyl-CoA with C16 fatty acid chains (PubMed:29326245). Can also utilize acyl-CoA with C14 and C18 fatty acid chains (PubMed:29326245).[UniProtKB/Swiss-Prot Function]